Exam 9: DNA-Based Information Technologies

Chimpanzees and humans differ by approximately how much due to transposons, base-pair changes, and chromosome segment duplications and rearrangements across their entire genomic sequences?

If you are comparing the total gene expression of a black panther during its early development and later adult life and have labeled the cDNA you obtained from its early development stages with a green fluorophore and the cDNA you obtained from its adult life stages with a red fluorophore, what color would you expect to see on your DNA microarray for genes that are equally expressed in both the early development and adult life stages?

The gene for green fluorescent protein was originally isolated from which species?

_____ is NOT a commonly used tag for affinity purification of cloned proteins.

Which statement does NOT apply to the construction or use of a DNA library?

Briefly explain the procedure used in next-generation pyrosequencing.

Which restriction endonuclease cuts DNA in a way that leaves blunt ends?

Which DNA sequence is a palindrome?

If you wanted to clone a large fragment of DNA into a unicellular eukaryotic host, what would you use?

The biochemical activity of a protein, such as its enzymatic activity, is called its _____ function.

Name one enzyme that is always used to make a cDNA library but is generally not used to make a genomic DNA library. Describe its function briefly.

If you wanted to create specific DNA sequence changes to a plasmid in the coding sequence for a protein you are researching, but there are no suitably located restriction sites nearby, which technique would you use?

Which type of organism do you NOT expect to express functionally fluorescent GFP?

To make an DNA copy of a RNA sequence, you need the reverse transcriptase enzyme and an oligonucleotide primer made of which nucleotide?

Which technique can be used to estimate the relative copy numbers of particular DNA sequences in a sample?

Especially stable haplotypes exist in which portions of the human genome due to their relative lack of meiotic recombination?

Describe how the CRISPR/Cas9 system is used to inactivate a gene during the process of genomic engineering.

When bacterial artificial chromosomes (BACs) are used as cloning vectors, what size of DNA fragment can be cloned?

Compensatory mutations in genes for noncoding RNAs indicate that:

Which statement about the polymerase chain reaction (PCR) is FALSE?