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If You Wanted to Create Specific DNA Sequence Changes to a Plasmid

Question 72

Multiple Choice

If you wanted to create specific DNA sequence changes to a plasmid in the coding sequence for a protein you are researching, but there are no suitably located restriction sites nearby, which technique would you use?


A) error-prone PCR
B) site-directed mutagenesis
C) oligonucleotide-directed mutagenesis
D) exposing the plasmid DNA to UV light
E) exposing the cells containing the plasmid DNA to chemical mutagens

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