Question 1

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-The coenzyme ______ is the oxidizing agent in both aerobic and anaerobic glycolysis.

Accepted Answer

A) fructose-1,6-bisphosphatase 
B) pentose phosphate 
C) pyruvate decarboxylase 
D) hypoglycemia 
E) phosphofructokinase
F)NADP+
G)thiamine pyrophosphate
H)ATP
I)galactosemia
J)NAD+
K)fluoride ion
L)pyruvate
M)glucogenic
N)FAD 
A) fructose-1,6-bisphosphatase 
B) pentose phosphate 
C) pyruvate decarboxylase 
D) hypoglycemia 
E) phosphofructokinase
F)NADP+
G)thiamine pyrophosphate
H)ATP
I)galactosemia
J)NAD+
K)fluoride ion
L)pyruvate
M)glucogenic
N)FAD

Question 2

Use the following reactions to answer questions :
I. 1,3-Bisphosphoglycerate (BPG)  → 3-phosphoglycerate(3PG)
II. Glucose → glucose-6-phosphate (G6P)
III.2-Phosphoglycerate (2PG)  → 3-phosphoglycerate (3PG)
IV. Glycerol-3-phosphate (GAP)  → 1,3-Bisphosphoglycerate (BPG)
V.Glucose-6-phosphate (G6P) →  fructose-6-phosphate (F6P)

-In which reaction above is ATP required?

Accepted Answer

A) I, II 
B) II only 
C) III only 
D) IV, V 
E) None of the reactions I-V requires ATP. 
A) I, II 
B) II only 
C) III only 
D) IV, V 
E) None of the reactions I-V requires ATP.

Question 3

Which of the following best explains why an enzyme hasn't evolved to catalyze the following reaction as the first step of glycolysis? 
glucose + Pi -> glucose-6-phosphate
I.The use of ATP is required for this step to serve as a regulatory control point.
II.The use of ATP is important because it helps keep its concentration low.Otherwise the high [ATP] would slow glycolysis too much through feedback inhibition.
III.The large positive $\Delta$G˚' of this reaction would need to be overcome by an exceedingly high [Pi].
IV.Inorganic phosphate cannot be specifically placed on the carbon in the 6th position.

Accepted Answer

A) I, III 
B) I, II 
C) II, IV 
D) I only 
E) III only 
A) I, III 
B) I, II 
C) II, IV 
D) I only 
E) III only

Question 4

Which glycolytic enzyme catalyzes a reaction with an enediolate intermediate?

Accepted Answer

A) phosphoglucomutase 
B) triose phosphate isomerase 
C) phosphofructokinase 
D) aldolase 
E) none of the above 
A) phosphoglucomutase 
B) triose phosphate isomerase 
C) phosphofructokinase 
D) aldolase 
E) none of the above

Question 5

Phosphofructokinase is allosterically _____ by high concentrations of _____.
I.activated; ATP
II.inhibited; ATP
III.inhibited; fructose-2,6-bisphosphate
IV.activated; fructose -2,6-bisphosphate

Accepted Answer

A) I, III 
B) II, III 
C) II, IV 
D) I, IV 
E) none of the above 
A) I, III 
B) II, III 
C) II, IV 
D) I, IV 
E) none of the above

Question 6

Use the following reactions to answer questions :
I. 1,3-Bisphosphoglycerate (BPG)  → 3-phosphoglycerate(3PG)
II. Glucose → glucose-6-phosphate (G6P)
III.2-Phosphoglycerate (2PG)  → 3-phosphoglycerate (3PG)
IV. Glycerol-3-phosphate (GAP)  → 1,3-Bisphosphoglycerate (BPG)
V.Glucose-6-phosphate (G6P) →  fructose-6-phosphate (F6P)
-Which reaction above is an oxidation reaction?

Accepted Answer

A) I, III 
B) II, III 
C) III only 
D) IV only 
E) None of the reactions I-V is oxidation reactions. 
A) I, III 
B) II, III 
C) III only 
D) IV only 
E) None of the reactions I-V is oxidation reactions.

Question 7

Conversion of galactose to glucose
I.requires an epimerase.
II.requires uridine diphosphate.
III.requires a transferase
IV.requires an isomerase.

Accepted Answer

A) I, II, III, IV 
B) I, II, III 
C) II, III, IV 
D) II, III 
E) I, III 
A) I, II, III, IV 
B) I, II, III 
C) II, III, IV 
D) II, III 
E) I, III

Question 8

Which of the following is true regarding glycolysis?
I.There is a net oxidation of substrate carbon atoms.
II.There is no net release of free energy.
III.Pyruvate is generated only during aerobic glycolysis.
IV.There is 2 ATP formed for every 2 glucose molecules.

Accepted Answer

A) I only 
B) I, IV 
C) I, III, IV 
D) IV only 
E) II, IV 
A) I only 
B) I, IV 
C) I, III, IV 
D) IV only 
E) II, IV

Question 9

Glycolytic synthesis of ATP occurs via

Accepted Answer

A) oxidation. 
B) reduction. 
C) substrate-level phosphorylation. 
D) oxidative phosphorylation. 
E) photophosphorylation. 
A) oxidation. 
B) reduction. 
C) substrate-level phosphorylation. 
D) oxidative phosphorylation. 
E) photophosphorylation.

Question 10

Matching 
Choose the correct answer from the list. Not all the answers will be used.
-The enzyme _____ is the major control point for glycolysis in muscle.

Accepted Answer

A) fructose-1,6-bisphosphatase 
B) pentose phosphate 
C) pyruvate decarboxylase 
D) hypoglycemia 
E) phosphofructokinase
F)NADP+
G)thiamine pyrophosphate
H)ATP
I)galactosemia
J)NAD+
K)fluoride ion
L)pyruvate
M)glucogenic
N)FAD 
A) fructose-1,6-bisphosphatase 
B) pentose phosphate 
C) pyruvate decarboxylase 
D) hypoglycemia 
E) phosphofructokinase
F)NADP+
G)thiamine pyrophosphate
H)ATP
I)galactosemia
J)NAD+
K)fluoride ion
L)pyruvate
M)glucogenic
N)FAD

Question 11

Of the reaction types listed below, which type of reaction is NOT used in glycolysis?

Accepted Answer

A) oxidation 
B) phosphorylation 
C) dehydration 
D) isomerization 
E) All are used in glycolysis. 
A) oxidation 
B) phosphorylation 
C) dehydration 
D) isomerization 
E) All are used in glycolysis.

Question 12

Which of the following best describes the entry of mannose into glycolysis?

Accepted Answer

A) hydrolyzed into two glucose units, they enter normally 
B) phosphorylated at C-1; UMP moiety transferred from UDP-glucose; epimerized at C-2; released from UMP moiety on next transfer as G1P 
C) phosphorylated at C-1; UMP moiety transferred from UDP-glucose; epimerized at C-4; released from UMP moiety on next transfer as G1P 
D) phosphorylated at C-6; epimerized at C-2 to produce G6P 
E) phosphorylated at C-6; isomerized to produce F6P 
A) hydrolyzed into two glucose units, they enter normally 
B) phosphorylated at C-1; UMP moiety transferred from UDP-glucose; epimerized at C-2; released from UMP moiety on next transfer as G1P 
C) phosphorylated at C-1; UMP moiety transferred from UDP-glucose; epimerized at C-4; released from UMP moiety on next transfer as G1P 
D) phosphorylated at C-6; epimerized at C-2 to produce G6P 
E) phosphorylated at C-6; isomerized to produce F6P

Question 13

If glucose labeled at the C-1 position with 14C passes through glycolysis, on which carbon of pyruvate will the radiolabel be found?

Accepted Answer

A) 1 
B) 2 
C) 3 
D) It will be released in CO2 rather than present in pyruvate. 
E) Not enough information is given to predict. 
A) 1 
B) 2 
C) 3 
D) It will be released in CO2 rather than present in pyruvate. 
E) Not enough information is given to predict.

Question 14

Which of the following compounds contains a "high-energy" bond and is used to produce ATP by substrate-level phosphorylation in glycolysis?

Accepted Answer

A)    
B)    
C)    
D)    
E)    
A)    
B)    
C)    
D)    
E)

Question 15

Experimental evidence indicates that glyceraldehyde-3-phosphate dehydrogenase contains a critical ______ residue in its active site, as shown by its inactivation by iodoacetamide.

Accepted Answer

A) alanine 
B) aspartate 
C) cysteine 
D) methionine 
E) lysine 
A) alanine 
B) aspartate 
C) cysteine 
D) methionine 
E) lysine

Question 16

Use the following reactions to answer questions :
I. 1,3-Bisphosphoglycerate (BPG)  → 3-phosphoglycerate(3PG)
II. Glucose → glucose-6-phosphate (G6P)
III.2-Phosphoglycerate (2PG)  → 3-phosphoglycerate (3PG)
IV. Glycerol-3-phosphate (GAP)  → 1,3-Bisphosphoglycerate (BPG)
V.Glucose-6-phosphate (G6P) →  fructose-6-phosphate (F6P)
-In which reaction above is at least 1 NADH formed?

Accepted Answer

A) II only 
B) III only 
C) IV only 
D) IV, V 
E) None of the reaction I-V form NADH. 
A) II only 
B) III only 
C) IV only 
D) IV, V 
E) None of the reaction I-V form NADH.

Question 17

In alcoholic fermentation, acetaldehyde is produced by

Accepted Answer

A) the decarboxylation of pyruvate. 
B) the carboxylation of pyruvate. 
C) the decarboxylation of lactate. 
D) the carboxylation of lactate. 
E) none of the above 
A) the decarboxylation of pyruvate. 
B) the carboxylation of pyruvate. 
C) the decarboxylation of lactate. 
D) the carboxylation of lactate. 
E) none of the above

Question 18

The metabolism of mannose requires _____ to produce ____ for entry into the glycolytic pathway.

Accepted Answer

A) hexokinase; glucose-6-phosphate 
B) hexokinase; fructose-6-phosphate 
C) phosphomannose isomerase; glucose-6-phosphate 
D) phosphomannose isomerase; fructose-6-phosphate 
E) phosphofructokinase; bisphosphoglycerate 
A) hexokinase; glucose-6-phosphate 
B) hexokinase; fructose-6-phosphate 
C) phosphomannose isomerase; glucose-6-phosphate 
D) phosphomannose isomerase; fructose-6-phosphate 
E) phosphofructokinase; bisphosphoglycerate

Question 19

During reactions utilizing the enzymes shown below, in which case(s)is ATP produced?
I.phosphofructokinase (PFK)
II.phosphoglycerate kinase (PGK)
III.pyruvate kinase (PK)

Accepted Answer

A) I 
B) II 
C) III 
D) I, II 
E) II, III 
A) I 
B) II 
C) III 
D) I, II 
E) II, III

Question 20

The reaction below is catalyzed by yeast alcohol dehydrogenase.Which of the following corresponds to X and Y in this reaction?

Accepted Answer

A) X = NADH+    ,Y = NAD + H+ 
B) X = NAD+   ,Y = NADH + H+ 
C) X = NADH + H+  , Y = NAD+ 
D) X = NAD + H+   ,Y = NADH+ 
E) X = NADPH + H+   ,Y = NADP+ 
A) X = NADH+    ,Y = NAD + H+ 
B) X = NAD+   ,Y = NADH + H+ 
C) X = NADH + H+  , Y = NAD+ 
D) X = NAD + H+   ,Y = NADH+ 
E) X = NADPH + H+   ,Y = NADP+

Exam 16: Glucose Catabolism

Matching Choose the correct answer from the list. Not all the answers will be used. -The coenzyme ______ is the oxidizing agent in both aerobic and anaerobic glycolysis.

Which glycolytic enzyme catalyzes a reaction with an enediolate intermediate?

Phosphofructokinase is allosterically _____ by high concentrations of _____. I.activated; ATP II.inhibited; ATP III.inhibited; fructose-2,6-bisphosphate IV.activated; fructose -2,6-bisphosphate

Conversion of galactose to glucose I.requires an epimerase. II.requires uridine diphosphate. III.requires a transferase IV.requires an isomerase.

Which of the following is true regarding glycolysis? I.There is a net oxidation of substrate carbon atoms. II.There is no net release of free energy. III.Pyruvate is generated only during aerobic glycolysis. IV.There is 2 ATP formed for every 2 glucose molecules.

Glycolytic synthesis of ATP occurs via

Matching Choose the correct answer from the list. Not all the answers will be used. -The enzyme _____ is the major control point for glycolysis in muscle.

Of the reaction types listed below, which type of reaction is NOT used in glycolysis?

Which of the following best describes the entry of mannose into glycolysis?

If glucose labeled at the C-1 position with 14C passes through glycolysis, on which carbon of pyruvate will the radiolabel be found?

Which of the following compounds contains a "high-energy" bond and is used to produce ATP by substrate-level phosphorylation in glycolysis?

Experimental evidence indicates that glyceraldehyde-3-phosphate dehydrogenase contains a critical ______ residue in its active site, as shown by its inactivation by iodoacetamide.

In alcoholic fermentation, acetaldehyde is produced by

The metabolism of mannose requires _____ to produce ____ for entry into the glycolytic pathway.

During reactions utilizing the enzymes shown below, in which case(s)is ATP produced? I.phosphofructokinase (PFK) II.phosphoglycerate kinase (PGK) III.pyruvate kinase (PK)

The reaction below is catalyzed by yeast alcohol dehydrogenase.Which of the following corresponds to X and Y in this reaction?

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters

Phosphofructokinase is allosterically _ by high concentrations of _. I.activated; ATP II.inhibited; ATP III.inhibited; fructose-2,6-bisphosphate IV.activated; fructose -2,6-bisphosphate

If glucose labeled at the C-1 position with ¹⁴C passes through glycolysis, on which carbon of pyruvate will the radiolabel be found?

The metabolism of mannose requires _ to produce for entry into the glycolytic pathway.