Question 1

What test method is used to detect antibodies to Mycoplasma spp.?

Accepted Answer

A) Complement fixation (CF) 
B) Enzyme immunoassay (EI
A)  
C) Direct fluorescent antibody (DF
A)  
D) Cellulose ion exchange (CI
E)  
A) Complement fixation (CF) 
B) Enzyme immunoassay (EI
A)  
C) Direct fluorescent antibody (DF
A)  
D) Cellulose ion exchange (CI
E)  B

Question 2

What two organisms are associated with urogenital tract infections but have also been isolated from asymptomatic individuals?

Accepted Answer

A) Mycoplasma hominis and Ureaplasma urealyticum 
B) M.hominis and M.fermentans 
C) U.urealyticum and M.pneumoniae 
D) M.penetrans and M.pneumoniae 
A) Mycoplasma hominis and Ureaplasma urealyticum 
B) M.hominis and M.fermentans 
C) U.urealyticum and M.pneumoniae 
D) M.penetrans and M.pneumoniae A

Question 3

Mycoplasma organisms will grow in which of the following atmospheres?

Accepted Answer

A) Ambient 
B) Capnophilic 
C) Anaerobic 
D) All of the above 
A) Ambient 
B) Capnophilic 
C) Anaerobic 
D) All of the above D

Question 4

Cultures for Mycoplasma pneumoniae should be incubated for:

Accepted Answer

A) 2 to 3 days. 
B) 5 to 7 days. 
C) 10 to 14 days. 
D) 21 days or more. 
A) 2 to 3 days. 
B) 5 to 7 days. 
C) 10 to 14 days. 
D) 21 days or more.

Question 5

When transporting specimens for Mycoplasma culture to the laboratory, extreme care must be taken so that the specimen does not:

Accepted Answer

A) become contaminated. 
B) leak from the specimen container. 
C) become overgrown with bacterial normal flora. 
D) dry out. 
A) become contaminated. 
B) leak from the specimen container. 
C) become overgrown with bacterial normal flora. 
D) dry out.

Question 6

Which of the following methods is used to determine the presence of growth in mycoplasma broth media?

Accepted Answer

A) Broth turbidity 
B) Presence of biofilm on surface 
C) Change in phenol red pH indicator 
D) Release of radiolabeled CO2 
A) Broth turbidity 
B) Presence of biofilm on surface 
C) Change in phenol red pH indicator 
D) Release of radiolabeled CO2

Question 7

What time frame is suitable for blood draws for serologic testing to diagnose Mycoplasma infections?

Accepted Answer

A) Onset of symptoms and 2 to 3 weeks later 
B) Week after symptoms start and 2 months later 
C) Weeks after symptoms start and 3 months later 
D) Onset of symptoms and 2 months later 
A) Onset of symptoms and 2 to 3 weeks later 
B) Week after symptoms start and 2 months later 
C) Weeks after symptoms start and 3 months later 
D) Onset of symptoms and 2 months later

Question 8

If a newborn has symptoms of meningitis but the cerebrospinal fluid (CSF) is negative for both Gram stain and a routine culture, what organisms should be suspected?

Accepted Answer

A) Neisseria gonorrhea and Chlamydia trachomatis 
B) Treponema pallidum and Borrelia burgdorferi 
C) Mycoplasma hominis and Ureaplasma urealyticum 
D) M.pneumoniae and C.trachomatis 
A) Neisseria gonorrhea and Chlamydia trachomatis 
B) Treponema pallidum and Borrelia burgdorferi 
C) Mycoplasma hominis and Ureaplasma urealyticum 
D) M.pneumoniae and C.trachomatis

Question 9

Which antibiotic is added to mycoplasma isolation and transport media to minimize bacterial contamination?

Accepted Answer

A) Tetracycline 
B) Penicillin 
C) Erythromycin 
D) Gentamicin 
A) Tetracycline 
B) Penicillin 
C) Erythromycin 
D) Gentamicin

Question 10

At-risk groups for contracting walking pneumonia include all the following except:

Accepted Answer

A) prisoners. 
B) college students. 
C) military personnel. 
D) preschool children. 
A) prisoners. 
B) college students. 
C) military personnel. 
D) preschool children.

Question 11

Which of the following organisms was once called "T-strain mycoplasma?"

Accepted Answer

A) Ureaplasma spp. 
B) Mycoplasma hominis 
C) Mycoplasma fermentans 
D) Mycoplasma orale 
A) Ureaplasma spp. 
B) Mycoplasma hominis 
C) Mycoplasma fermentans 
D) Mycoplasma orale

Question 12

One of the factors that allows Mycoplasma to infect the respiratory tract and the urogenital tract is that:

Accepted Answer

A) the body has a hard time detecting their presence, because they have no cell wall. 
B) they are virulent organisms. 
C) they adhere to the epithelium of mucosal surfaces and are not eliminated by mucus secretions. 
D) all of the above. 
A) the body has a hard time detecting their presence, because they have no cell wall. 
B) they are virulent organisms. 
C) they adhere to the epithelium of mucosal surfaces and are not eliminated by mucus secretions. 
D) all of the above.

Question 13

When Mycoplasma hominis invades the upper genitourinary tract, it can cause:

Accepted Answer

A) salpingitis. 
B) pyelonephritis. 
C) pelvic inflammatory disease. 
D) all of the above. 
A) salpingitis. 
B) pyelonephritis. 
C) pelvic inflammatory disease. 
D) all of the above.

Question 14

What disease causes the condition primary atypical pneumonia?

Accepted Answer

A) Mycoplasma hominis 
B) M.urealyticum 
C) M.fermentans 
D) M.pneumoniae 
A) Mycoplasma hominis 
B) M.urealyticum 
C) M.fermentans 
D) M.pneumoniae

Question 15

Mycoplasmas are a different type of bacteria because they do not have a:

Accepted Answer

A) cell wall. 
B) cell membrane. 
C) nuclear membrane. 
D) messenger transporter. 
A) cell wall. 
B) cell membrane. 
C) nuclear membrane. 
D) messenger transporter.

Question 16

Mycoplasma spp.from patient samples are visible by which staining technique?

Accepted Answer

A) Gram stain 
B) Wright's stain 
C) Acridine orange 
D) All of the above 
A) Gram stain 
B) Wright's stain 
C) Acridine orange 
D) All of the above

Question 17

All the following antibiotics are used to treat mycoplasma infections except:

Accepted Answer

A) macrolides. 
B) fluoroquinolones. 
C) tetracyclines. 
D) penicillin. 
A) macrolides. 
B) fluoroquinolones. 
C) tetracyclines. 
D) penicillin.

Question 18

What organism has been recovered in normal, sexually active females but is also associated with chorioamnionitis, congenital pneumonia, and development of chronic lung disease in premature infants?

Accepted Answer

A) Mycoplasma hominis 
B) Ureaplasma ureolyticus 
C) M.penetrans 
D) M.fermentans 
A) Mycoplasma hominis 
B) Ureaplasma ureolyticus 
C) M.penetrans 
D) M.fermentans

Question 19

Which factor is added to media to provide cholesterol for mycoplasma-like organisms?

Accepted Answer

A) Trypticase soy digest 
B) 20% fetal calf serum 
C) X and V factor supplement 
D) 5% sheep blood 
A) Trypticase soy digest 
B) 20% fetal calf serum 
C) X and V factor supplement 
D) 5% sheep blood

Question 20

Which of the following stains is used to enhance the visibility of mycoplasma-like colonies?

Accepted Answer

A) Gram stain 
B) Acridine orange 
C) Dienes stain 
D) Wright's stain 
A) Gram stain 
B) Acridine orange 
C) Dienes stain 
D) Wright's stain

Exam 25: Mycoplasma and Ureaplasma

What test method is used to detect antibodies to Mycoplasma spp.?

What two organisms are associated with urogenital tract infections but have also been isolated from asymptomatic individuals?

Mycoplasma organisms will grow in which of the following atmospheres?

Cultures for Mycoplasma pneumoniae should be incubated for:

When transporting specimens for Mycoplasma culture to the laboratory, extreme care must be taken so that the specimen does not:

Which of the following methods is used to determine the presence of growth in mycoplasma broth media?

What time frame is suitable for blood draws for serologic testing to diagnose Mycoplasma infections?

If a newborn has symptoms of meningitis but the cerebrospinal fluid (CSF) is negative for both Gram stain and a routine culture, what organisms should be suspected?

Which antibiotic is added to mycoplasma isolation and transport media to minimize bacterial contamination?

At-risk groups for contracting walking pneumonia include all the following except:

Which of the following organisms was once called "T-strain mycoplasma?"

One of the factors that allows Mycoplasma to infect the respiratory tract and the urogenital tract is that:

When Mycoplasma hominis invades the upper genitourinary tract, it can cause:

What disease causes the condition primary atypical pneumonia?

Mycoplasmas are a different type of bacteria because they do not have a:

Mycoplasma spp.from patient samples are visible by which staining technique?

All the following antibiotics are used to treat mycoplasma infections except:

What organism has been recovered in normal, sexually active females but is also associated with chorioamnionitis, congenital pneumonia, and development of chronic lung disease in premature infants?

Which factor is added to media to provide cholesterol for mycoplasma-like organisms?

Which of the following stains is used to enhance the visibility of mycoplasma-like colonies?

Bacterial Cell Structure, Physiology, Metabolism, and Genetics

Host-Parasite Interaction

The Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Specimen Collection and Processing

Microscopic Examination of Materials From Infected Sites

Use of Colony Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

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Antimicrobial Agent Mechanisms of Action and Resistance

Antimicrobial Susceptibility Testing

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Streptococcus, Enterococcus, and Other Catalase-Negative, Gram-Positive Cocci

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Haemophilus and Other Fastidious Gram-Negative Bacilli

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Vibrio, Aeromonas, Plesiomonas, and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

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Chlamydia, Rickettsia and Similar Organisms

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Medically Significant Fungi

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Biofilms: Architects of Disease

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Gastrointestinal Infections and Food Poisoning

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