Exam 9: Biochemical Identification of Gram-Negative Bacteria

When performing the oxidative/fermentative (O/F) test, one tube is covered with mineral oil and one tube is left uncovered.Why is one tube covered with mineral oil?

What two reagents are needed to visualize nitrite in the nitrate reduction test?

Commercial identification systems fall into all of the following categories except:

The two reagents used in the Voges-Proskauer test are:

Nucleic acid assays are based on the _____ of the organism and are believed to be more accurate.

A technician is reading the biochemical tests for identifying a particular gram-negative rod.The organism has produced acid, indicated by a color change, in the closed tube only.This indicates that the organism is:

What amino acid is the substrate in indole test broth?

The biochemical pathway that the methyl red test confirms is:

What two reagents can be used to visualize indole in the broth?

During this process, glucose enters the glycolysis pathway, resulting in the formation of pyruvic acid, which is further oxidized to other acids.What is this process called?

What advantage do the methods based on enzyme substrates have over conventional methods?

Using Moeller's decarboxylase base medium, what color is a positive reaction and what causes this color to be produced?

The methyl red test detects metabolic by-products from what pathway?

What type of medium is used in the motility test?

If an organism fermented glucose only and utilized peptones, what would the triple sugar iron (TSI) reaction be?

What medium is used in the urease test?

The Vitek AutoMicrobic System is manufactured by:

What does a lysine iron agar (LIA) slant contain?

This identification system for Enterobacteriaceae has a series of 20 cupules attached to a plastic strip.Inside the cupules are lyophilized pH-based substrates.What is the name of this system?

What chemicals are added to triple sugar iron (TSI) to detect the production of hydrogen sulfide gas?