Exam 12: Biotechniques and Synthetic Biology

Genes coding for insecticidal peptides have been engineered into the genomes of some crops. Which of these peptides are naturally produced by the bacterium?

Scientists have taken the genome of the bacterial species ________ and moved it to yeast.

The migration of linear DNA molecules in agarose or polyacrylamide gels occurs at a rate that is

In the first stage of the western blot procedure, a technique known as SDS-PAGE is used to separate proteins according to their

Annotation of sequences of transposon insertion sites in the chromosome of Escherichia coli O157:H7 cells that had lost their acid-resistance properties revealed four genes: two encoding isoforms of glutamate decarboxylase (GadA and GadB) and one encoding a glutamate/gamma-aminobutyric acid (GABA) antiporter. What does the fourth gene encode?

In a DNA protection assay gel, it is necessary to run sequencing ladders of the putative protein-binding DNA region along with DNase I-treated samples in order to

Observe the figure below and discuss how a single northern blot can reveal differences in size and abundance of the E. coli acid-resistance gene gadA with respect to the gadBC operon and the inducible expression of these genes at low pH.

A common approach to study a certain metabolic process is to isolate organisms defective in that particular process. These organisms are called

Discuss possible disadvantages of the yeast two-hybrid system.

Describe the whole-genome DNA-binding analysis using ChIP-on-chip technology.

Describe the use of a primary and secondary antibody in a western blot.

Potential vaccine antigens made by transgenic plants include