Question 1

How is polyagglutination resolved?

Accepted Answer

A)  Absorption of Tn antigen with anti-T 
B)  Patient serum is tested with a lectin panel 
C)  Patient cells are tested with a lectin panel 
D)  Elution of Tn antigen with chloroquine 
A)  Absorption of Tn antigen with anti-T 
B)  Patient serum is tested with a lectin panel 
C)  Patient cells are tested with a lectin panel 
D)  Elution of Tn antigen with chloroquine

Question 2

An AB male mates with an AB female. What could be the genotype of the offspring?

Accepted Answer

A)  AB 
B)  AA 
C)  BB 
D)  All of the above 
A)  AB 
B)  AA 
C)  BB 
D)  All of the above

Question 3

Why is reverse grouping not performed on cord blood specimens?

Accepted Answer

A)  Antigens are not present at birth. 
B)  Antibodies are generally not present at birth. 
C)  Antibody titer is too high. 
D)  Antigens are too weak. 
A)  Antigens are not present at birth. 
B)  Antibodies are generally not present at birth. 
C)  Antibody titer is too high. 
D)  Antigens are too weak.

Question 4

All of the following statements are true concerning ABH soluble substances except:

Accepted Answer

A)  the first sugar in the precursor substance is N-acetylgalactosamine. 
B)  the precursor chain is type 2 (beta 1-4 linkage). 
C)  ABH structures are glycoproteins. 
D)  L-fucosyltransferase production is regulated by the Se system. 
A)  the first sugar in the precursor substance is N-acetylgalactosamine. 
B)  the precursor chain is type 2 (beta 1-4 linkage). 
C)  ABH structures are glycoproteins. 
D)  L-fucosyltransferase production is regulated by the Se system.

Question 5

What immunodominant sugar is responsible for B specificity?

Accepted Answer

A)  D-galactose 
B)  L-fucose 
C)  N-acetyl-D-galactosamine 
D)  D-glucose 
A)  D-galactose 
B)  L-fucose 
C)  N-acetyl-D-galactosamine 
D)  D-glucose

Question 6

What percentage of the white population has type-O blood?

Accepted Answer

A)  45% 
B)  10% 
C)  4% 
D)  32% 
A)  45% 
B)  10% 
C)  4% 
D)  32%

Question 7

All of the following are tests performed in the blood bank to classify subgroups of A except:

Accepted Answer

A)  anti-H lectin reactivity. 
B)  absorption. 
C)  LISS enhancement. 
D)  elution. 
A)  anti-H lectin reactivity. 
B)  absorption. 
C)  LISS enhancement. 
D)  elution.

Question 8

When performing secretor studies, what is omitted in the control tube but present in the patient tube?

Accepted Answer

A)  Antiserum 
B)  5% B cells 
C)  Saliva 
D)  5% O cells 
A)  Antiserum 
B)  5% B cells 
C)  Saliva 
D)  5% O cells

Question 9

A patient was previously typed as blood group O. Forward grouping was negative with anti-A and anti-B. Reverse grouping showed reactivity with A1 cells and B cells. The technologist reported this patient's type as A. What technical error occurred?

Accepted Answer

A)  Sample misidentified 
B)  Failure to add reagents 
C)  Clerical error 
D)  Sample mix-up 
A)  Sample misidentified 
B)  Failure to add reagents 
C)  Clerical error 
D)  Sample mix-up

Question 10

What testing is available that will differentiate between a true B and an acquired B?

Accepted Answer

A)  Anti-B lectin 
B)  Acidification of anti-B reagent 
C)  Secretor studies 
D)  All of the above 
A)  Anti-B lectin 
B)  Acidification of anti-B reagent 
C)  Secretor studies 
D)  All of the above

Question 11

What is used to stimulate saliva secretion in secretor studies?

Accepted Answer

A)  Tongue depressor 
B)  Paraffin wax 
C)  Chewing gum 
D)  Water 
A)  Tongue depressor 
B)  Paraffin wax 
C)  Chewing gum 
D)  Water

Question 12

The forward grouping of a patient showed no agglutination of patient cells with anti-A, anti-B, or anti-AB reagent antisera. The reverse grouping showed agglutination with A1 and B cells. What is this person's ABO group?

Accepted Answer

A)  A 
B)  B 
C)  AB 
D)  O 
A)  A 
B)  B 
C)  AB 
D)  O

Question 13

A group O person with warm autoimmune hemolytic anemia may demonstrate weak reactions in the forward grouping due to:

Accepted Answer

A)  red blood cells being coated with antigen. 
B)  red blood cells being coated with antibody. 
C)  exposure of T antigen. 
D)  acquired B-like syndrome. 
A)  red blood cells being coated with antigen. 
B)  red blood cells being coated with antibody. 
C)  exposure of T antigen. 
D)  acquired B-like syndrome.

Question 14

What other consideration should be made before beginning a subgroup investigation?

Accepted Answer

A)  Patient demographics 
B)  ABH alteration caused by malignancy 
C)  Chromosomal aberration 
D)  Immunization record of patient 
A)  Patient demographics 
B)  ABH alteration caused by malignancy 
C)  Chromosomal aberration 
D)  Immunization record of patient

Question 15

Which blood group contains the highest concentration of H antigen?

Accepted Answer

A)  A2 
B)  B 
C)  AB 
D)  O 
A)  A2 
B)  B 
C)  AB 
D)  O

Question 16

Which of the following criteria is used to classify the B subgroups?

Accepted Answer

A)  Agglutination patterns with anti-B, anti-AB, and anti-H 
B)  Presence of ABH isoagglutinins in serum 
C)  Absorption studies with anti-B 
D)  All of the above 
A)  Agglutination patterns with anti-B, anti-AB, and anti-H 
B)  Presence of ABH isoagglutinins in serum 
C)  Absorption studies with anti-B 
D)  All of the above

Question 17

Reverse grouping showed negative reactions with A1 and B cells. Forward grouping showed positive reactions with A, B, and AB antisera. What blood type is consistent with these results?

Accepted Answer

A)  A 
B)  B 
C)  AB 
D)  O 
A)  A 
B)  B 
C)  AB 
D)  O

Question 18

Antibody titers specific to antigens from the ABO system are typically highest:

Accepted Answer

A)  when the patient is a newborn. 
B)  when the patient is around 10 years old. 
C)  when the patient is around 30 years old. 
D)  when the patient is around 60 years old. 
A)  when the patient is a newborn. 
B)  when the patient is around 10 years old. 
C)  when the patient is around 30 years old. 
D)  when the patient is around 60 years old.

Question 19

Why can anti-H sometimes be found in an A1B individual?

Accepted Answer

A)  L-fucose does not attach to precursor substance. 
B)  The H gene is not inherited. 
C)  The specific immunodominant sugar blocks the presence of H antigen. 
D)  The individual does not secrete H substance in secretions. 
A)  L-fucose does not attach to precursor substance. 
B)  The H gene is not inherited. 
C)  The specific immunodominant sugar blocks the presence of H antigen. 
D)  The individual does not secrete H substance in secretions.

Question 20

Approximately how many antigen sites exist on a type-A1 individual's RBC?

Accepted Answer

A)  1 million 
B)  600,000 
C)  1,000 
D)  5 million 
A)  1 million 
B)  600,000 
C)  1,000 
D)  5 million

Exam 6: The Abo Blood Group System

How is polyagglutination resolved?

An AB male mates with an AB female. What could be the genotype of the offspring?

Why is reverse grouping not performed on cord blood specimens?

All of the following statements are true concerning ABH soluble substances except:

What immunodominant sugar is responsible for B specificity?

What percentage of the white population has type-O blood?

All of the following are tests performed in the blood bank to classify subgroups of A except:

When performing secretor studies, what is omitted in the control tube but present in the patient tube?

A patient was previously typed as blood group O. Forward grouping was negative with anti-A and anti-B. Reverse grouping showed reactivity with A1 cells and B cells. The technologist reported this patient's type as A. What technical error occurred?

What testing is available that will differentiate between a true B and an acquired B?

What is used to stimulate saliva secretion in secretor studies?

The forward grouping of a patient showed no agglutination of patient cells with anti-A, anti-B, or anti-AB reagent antisera. The reverse grouping showed agglutination with A1 and B cells. What is this person's ABO group?

A group O person with warm autoimmune hemolytic anemia may demonstrate weak reactions in the forward grouping due to:

What other consideration should be made before beginning a subgroup investigation?

Which blood group contains the highest concentration of H antigen?

Which of the following criteria is used to classify the B subgroups?

Reverse grouping showed negative reactions with A1 and B cells. Forward grouping showed positive reactions with A, B, and AB antisera. What blood type is consistent with these results?

Antibody titers specific to antigens from the ABO system are typically highest:

Why can anti-H sometimes be found in an A1B individual?

Approximately how many antigen sites exist on a type-A1 individual's RBC?

Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends

Basic Genetics

Fundamentals of Immunology

Concepts in Molecular Biology

The Antiglobulin Test

The Rh Blood Group System

Blood Group Terminology and Common Blood Groups

Uncommon Blood Groups

Detection and Identification of Antibodies

Pre-Transfusion Testing

Blood Bank Technologies and Automation

Donor Selection

Transfusion-Transmitted Diseases

Component Preparation

Transfusion Therapy

Adverse Effects of Blood Transfusion

Apheresis

Cellular Therapy in the Transplant Setting

Hemolytic Disease of the Fetus and Newborn

Autoimmune Hemolytic Anemias

Tissue Banking As Part of the Transfusion Service

The Hla System

Relationship Testing

Quality Management

Patient Blood Management

Transfusion Safety and Federal Regulatory Requirements

Laboratory Information Systems in the Blood Bank

Medicolegal and Ethical Aspects of Providing Blood Collection and Transfusion Services

Filters

A patient was previously typed as blood group O. Forward grouping was negative with anti-A and anti-B. Reverse grouping showed reactivity with A₁ cells and B cells. The technologist reported this patient's type as A. What technical error occurred?

The forward grouping of a patient showed no agglutination of patient cells with anti-A, anti-B, or anti-AB reagent antisera. The reverse grouping showed agglutination with A₁ and B cells. What is this person's ABO group?

Reverse grouping showed negative reactions with A₁ and B cells. Forward grouping showed positive reactions with A, B, and AB antisera. What blood type is consistent with these results?

Why can anti-H sometimes be found in an A₁B individual?

Approximately how many antigen sites exist on a type-A₁ individual's RBC?