Question 1

The selectivity of aquaporin for H2O relies on all EXCEPT which of the following?

Accepted Answer

A)  hydrogen bonding to Asn 
B)  a 2.8 Å constriction point within the protein 
C)  inverted  -helix dipoles 
D)  electrostatic repulsion from Asn 
A)  hydrogen bonding to Asn 
B)  a 2.8 Å constriction point within the protein 
C)  inverted  -helix dipoles 
D)  electrostatic repulsion from Asn

Question 2

Which of the following accounts for some of the specificity of the K+ channel?

Accepted Answer

A)  The radius of solvated Na+ is smaller than the radius of solvated K+. 
B)  The number of  -strands that make up the channel. 
C)  The orientation of backbone carbonyl oxygen atoms in the channel. 
D)  Alternating positive and negative amino acids in the channel pore. 
A)  The radius of solvated Na+ is smaller than the radius of solvated K+. 
B)  The number of  -strands that make up the channel. 
C)  The orientation of backbone carbonyl oxygen atoms in the channel. 
D)  Alternating positive and negative amino acids in the channel pore.

Question 3

Radioactive iodine can be used in laboratory settings to label inhibitors.When working in a lab,a researcher is exposed to the radioactive iodine when some accidentally spills on her exposed wrist.A scan with a Geiger counter the next day indicates that most of the radioactivity is located in her neck.Predict how this localization occurred and include any transporters that may be involved.

Accepted Answer

Iodine is concentrated in the thyroid,wh

Question 4

The estrogen receptor is an example of a

Accepted Answer

A)  metabolic enzyme. 
B)  transport protein. 
C)  genomic caretaker protein. 
D)  cell signaling protein. 
A)  metabolic enzyme. 
B)  transport protein. 
C)  genomic caretaker protein. 
D)  cell signaling protein.

Question 5

Which of the following curves is representative of the binding of oxygen to myoglobin?

Accepted Answer

A)  curve A 
B)  curve B 
C)  curve C 
D)  curve D 
A)  curve A 
B)  curve B 
C)  curve C 
D)  curve D

Question 6

Predict the effect on Na+ transport through gramicidin A if the helical structure took on the conformation of a true   
-helix.

Accepted Answer

Na⁺ would no longer be transpor

Question 7

You design an experiment in which you can monitor the step-by-step movement of H+ and Ca2+ through the SERCA protein,starting with the protein in the E2-ATP state and moving through the transport cycle,ultimately returning to the E2-ATP state.Your control experiment includes everything required for the system to function normally,and you easily detect four distinct conformations of the protein.You then replace the ATP in the system with ATP   S,a nonhydrolyzable form of ATP,and begin the experiment again with the protein in the E2-ATP state.Predict which conformations of SERCA you will detect.

Accepted Answer

Only the E2-ATP conformation will be det

Question 8

A newly discovered transporter protein is being investigated using an assay to determine whether transport has occurred in the cells.A nonhydrolyzable form of ATP is added to the cells,and it is found that transport is reduced.Explain these results.

Accepted Answer

The transporter protein must b

Question 9

In the shift from the T to the R state in adult hemoglobin,a shift between the subunits occurs.Explain how this shift leads to the variation of binding affinity for 2,3-BPG in the two states.

Accepted Answer

The shift from the T state to the R stat

Question 10

Which of the following is a genomic caretaker protein?

Accepted Answer

A)  topoisomerase 
B)  insulin 
C)  myoglobin 
D)  tubulin 
A)  topoisomerase 
B)  insulin 
C)  myoglobin 
D)  tubulin

Question 11

Below is a figure of adult hemoglobin.The arrow points to where __________ binds.

Accepted Answer

A)  glucose 
B)  2,3-bisphosphoglycerate 
C)  O2 
D)  CO2 
A)  glucose 
B)  2,3-bisphosphoglycerate 
C)  O2 
D)  CO2

Question 12

Which of the following is NOT a passive transporter protein?

Accepted Answer

A)  porin 
B)  Cl- channel 
C)  Ca2+-ATPase transporter protein 
D)  Ca2+ channel 
A)  porin 
B)  Cl- channel 
C)  Ca2+-ATPase transporter protein 
D)  Ca2+ channel

Question 13

An increase in sickle cell disease symptoms can occur when a person with the disease is at higher altitudes.Hypothesize how allosteric regulation at high altitudes may be related to sickle cell anemia.

Accepted Answer

At high altitudes,more 2,3-bisphosphogly

Question 14

Which of the following statements is true of the data shown below?

Accepted Answer

A)  The binding of ligand to protein B follows the Bohr effect. 
B)  Both proteins display cooperative binding. 
C)  Protein A has a higher Kd for the ligand than protein B. 
D)  Protein B and protein A bind to the same ligand. 
A)  The binding of ligand to protein B follows the Bohr effect. 
B)  Both proteins display cooperative binding. 
C)  Protein A has a higher Kd for the ligand than protein B. 
D)  Protein B and protein A bind to the same ligand.

Question 15

Decide if the rate of malate transport by the Omp32 protein of Delftia acidovorans will be affected if the pH of the system is changed from 6.8 to 8.2.Explain your reasoning.
The pKa of amino acids with ionizable side chains are provided in the table below.Note that malic acid contains two ionizable groups with pKa of 3.40 and 5.20.Assume in this scenario that the pH change does not alter the overall conformation of the protein.

Accepted Answer

The rate will not be changed s

Question 16

Which of the following groups are hydrogen bonded in oxyhemoglobin but not deoxyhemoglobin?

Accepted Answer

A)  Asp94 and Asn102 
B)  Asp94 and Tyr42 
C)  Try42 and Asp99 
D)  Asp99 and Asn102 
A)  Asp94 and Asn102 
B)  Asp94 and Tyr42 
C)  Try42 and Asp99 
D)  Asp99 and Asn102

Question 17

Using the data shown,list the porins from the most selective to the least selective.

Accepted Answer

The answer of Using the data shown,list the porins from...

Question 18

What might be the effect on the transport of lactose into the cytosol of E.coli if a drug is added that allows H+ to freely pass through the inner bacterial membrane?

Accepted Answer

A)  There would be no effect. 
B)  Lactose transport would increase. 
C)  Lactose transport would cease. 
D)  Lactose would be transported out of the cytosol and into the periplasm. 
A)  There would be no effect. 
B)  Lactose transport would increase. 
C)  Lactose transport would cease. 
D)  Lactose would be transported out of the cytosol and into the periplasm.

Question 19

As K+ passes through the selectivity filter of the K+ channel of Streptomyces lividans it interacts with

Accepted Answer

A)  glycine side chains. 
B)  amino acid carbonyl groups. 
C)  tyrosine side chains. 
D)  amino acid amino groups. 
A)  glycine side chains. 
B)  amino acid carbonyl groups. 
C)  tyrosine side chains. 
D)  amino acid amino groups.

Question 20

Propose how the addition of an inhibitor of the permeability glycoprotein transporter to a chemotherapy drug regimen would alter the effectiveness of the regimen.

Accepted Answer

The permeability glycoprotein transporte

The selectivity of aquaporin for H₂O relies on all EXCEPT which of the following?

Which of the following accounts for some of the specificity of the K⁺ channel?

The estrogen receptor is an example of a

Which of the following curves is representative of the binding of oxygen to myoglobin?

Predict the effect on Na⁺ transport through gramicidin A if the helical structure took on the conformation of a true -helix.

A newly discovered transporter protein is being investigated using an assay to determine whether transport has occurred in the cells.A nonhydrolyzable form of ATP is added to the cells,and it is found that transport is reduced.Explain these results.

In the shift from the T to the R state in adult hemoglobin,a shift between the subunits occurs.Explain how this shift leads to the variation of binding affinity for 2,3-BPG in the two states.

Which of the following is a genomic caretaker protein?

Below is a figure of adult hemoglobin.The arrow points to where __________ binds.

Which of the following is NOT a passive transporter protein?

An increase in sickle cell disease symptoms can occur when a person with the disease is at higher altitudes.Hypothesize how allosteric regulation at high altitudes may be related to sickle cell anemia.

Which of the following statements is true of the data shown below?

Which of the following groups are hydrogen bonded in oxyhemoglobin but not deoxyhemoglobin?

Using the data shown,list the porins from the most selective to the least selective.

What might be the effect on the transport of lactose into the cytosol of E.coli if a drug is added that allows H⁺ to freely pass through the inner bacterial membrane?

As K⁺ passes through the selectivity filter of the K⁺ channel of Streptomyces lividans it interacts with

Propose how the addition of an inhibitor of the permeability glycoprotein transporter to a chemotherapy drug regimen would alter the effectiveness of the regimen.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

Exam 6: Protein Function

The selectivity of aquaporin for H2O relies on all EXCEPT which of the following?

Which of the following accounts for some of the specificity of the K+ channel?

The estrogen receptor is an example of a

Which of the following curves is representative of the binding of oxygen to myoglobin?

Predict the effect on Na+ transport through gramicidin A if the helical structure took on the conformation of a true -helix.

A newly discovered transporter protein is being investigated using an assay to determine whether transport has occurred in the cells.A nonhydrolyzable form of ATP is added to the cells,and it is found that transport is reduced.Explain these results.

In the shift from the T to the R state in adult hemoglobin,a shift between the subunits occurs.Explain how this shift leads to the variation of binding affinity for 2,3-BPG in the two states.

Which of the following is a genomic caretaker protein?

Below is a figure of adult hemoglobin.The arrow points to where __________ binds.

Which of the following is NOT a passive transporter protein?

An increase in sickle cell disease symptoms can occur when a person with the disease is at higher altitudes.Hypothesize how allosteric regulation at high altitudes may be related to sickle cell anemia.

Which of the following statements is true of the data shown below?

Which of the following groups are hydrogen bonded in oxyhemoglobin but not deoxyhemoglobin?

Using the data shown,list the porins from the most selective to the least selective.

What might be the effect on the transport of lactose into the cytosol of E.coli if a drug is added that allows H+ to freely pass through the inner bacterial membrane?

As K+ passes through the selectivity filter of the K+ channel of Streptomyces lividans it interacts with

Propose how the addition of an inhibitor of the permeability glycoprotein transporter to a chemotherapy drug regimen would alter the effectiveness of the regimen.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

The selectivity of aquaporin for H₂O relies on all EXCEPT which of the following?

Which of the following accounts for some of the specificity of the K⁺ channel?

Predict the effect on Na⁺ transport through gramicidin A if the helical structure took on the conformation of a true -helix.

What might be the effect on the transport of lactose into the cytosol of E.coli if a drug is added that allows H⁺ to freely pass through the inner bacterial membrane?

As K⁺ passes through the selectivity filter of the K⁺ channel of Streptomyces lividans it interacts with