Question 1

Consider the following interaction between a protein and its ligand: [P] + [L]   [PL].
If [L] = 5 mM,[P] = 3 mM,and [PL] = 2 mM,what is the Ka for the interaction?

Accepted Answer

A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM 
A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM

Question 2

Consider the following interaction between a protein and its ligand: [P] + [L]   [PL].If [L] = 5 mM,[P] = 3 mM,and [PL] = 2 mM,what is the dissociation constant?

Accepted Answer

A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM 
A)  7.5 mM 
B)  15 mM 
C)  0.133 mM 
D)  0.4 mM

Question 3

Consider an oxygen binding curve for hemoglobin at pH 7.4.The binding curve would shift __________ if the pH is reduced to 7.2.

Accepted Answer

A)  to the right 
B)  to the left 
C)  up 
D)  down 
A)  to the right 
B)  to the left 
C)  up 
D)  down

Question 4

Based on the data shown below,the Kd for protein A is __________ the Kd for protein B.

Accepted Answer

A)  equal to 
B)  approximately 50% greater than 
C)  six times lower than 
D)  six times higher than 
A)  equal to 
B)  approximately 50% greater than 
C)  six times lower than 
D)  six times higher than

Question 5

Which of the following is NOT an ABC transporter?

Accepted Answer

A)  cystic fibrosis transmembrane conductance regulator protein 
B)  SERCA 
C)  multidrug resistance protein 
D)  maltose transporter from E. coli 
A)  cystic fibrosis transmembrane conductance regulator protein 
B)  SERCA 
C)  multidrug resistance protein 
D)  maltose transporter from E. coli

Question 6

Thin filaments __________,but thick filaments do/are not.

Accepted Answer

A)  contain myosin 
B)  are directly attached to the Z disk 
C)  are part of the sarcomere 
D)  are part of the A band 
A)  contain myosin 
B)  are directly attached to the Z disk 
C)  are part of the sarcomere 
D)  are part of the A band

Question 7

Explain the relationship between P50 of oxygen binding and Kd for oxygen binding to myoglobin.

Accepted Answer

P50 is the amount of oxygen bound when h

Question 8

Articulate the difference between a myoblast and a myofibril.

Accepted Answer

A myoblast is a large fused cell that co

Question 9

Which of the following is a positive effector for oxygen binding to hemoglobin?

Accepted Answer

A)  CO2 
B)  O2 
C)  H+ 
D)  2,3-bisphosphoglycerate 
A)  CO2 
B)  O2 
C)  H+ 
D)  2,3-bisphosphoglycerate

Question 10

Construct a complete actin-myosin reaction cycle by placing the following steps in order,starting with Ca2+ binding to troponin:

Accepted Answer

A) ADP release empties the nucleotide binding site in myosin. 
B) Pi release induces the power stroke. 
C) ATP hydrolysis induces the recovery conformation. 
D) ATP binding causes myosin to dissociate from actin. 
A) ADP release empties the nucleotide binding site in myosin. 
B) Pi release induces the power stroke. 
C) ATP hydrolysis induces the recovery conformation. 
D) ATP binding causes myosin to dissociate from actin.

Question 11

A proposed structure of a bacterial membrane protein shows that the distance between successive amino acids in a region crossing the membrane is 3.4 Å.Recall that a typical lipid bilayer is approximately 40 Å across.Which of the following is the most likely description of the protein?

Accepted Answer

A)  The transmembrane region of the protein resembles the transmembrane region of the Ca2+-ATPase transporter protein. 
B)  The primary sequence of the transmembrane region of the protein is composed of approximately nine amino acids. 
C)  The protein contains a selectivity channel. 
D)  The protein resembles a porin. 
A)  The transmembrane region of the protein resembles the transmembrane region of the Ca2+-ATPase transporter protein. 
B)  The primary sequence of the transmembrane region of the protein is composed of approximately nine amino acids. 
C)  The protein contains a selectivity channel. 
D)  The protein resembles a porin.

Question 12

An E.coli strain is discovered that has a mutation in the gene encoding RecBCD.The mutation causes RecBCD to not function as effectively as the wild-type RecBCD.Hypothesize how the growth rate of this strain would compare with that of the wild type.

Accepted Answer

RecBCD is a genomic caretaker protein.It

Question 13

Hemoglobin contains __________   -helices,__________  
-sheets,and __________ globin folds.

Accepted Answer

A)  32; 4; 4 
B)  32; 0; 8 
C)  8; 8; 4 
D)  32; 0; 4 
A)  32; 4; 4 
B)  32; 0; 8 
C)  8; 8; 4 
D)  32; 0; 4

Question 14

Which of the following is true of sickle cell anemia?

Accepted Answer

A)  It is a dominant genetic disease. 
B)  It is caused by a mutation in the  -globin gene. 
C)  It is caused by an amino acid substitution in the F helix. 
D)  It results in a hemoglobin that contains a hydrophobic amino acid on the surface. 
A)  It is a dominant genetic disease. 
B)  It is caused by a mutation in the  -globin gene. 
C)  It is caused by an amino acid substitution in the F helix. 
D)  It results in a hemoglobin that contains a hydrophobic amino acid on the surface.

Question 15

Approximately how many amino acids are identical in both myoglobin and the adult   -subunit of hemoglobin?

Accepted Answer

A)  2% 
B)  20% 
C)  38% 
D)  81% 
A)  2% 
B)  20% 
C)  38% 
D)  81%

Question 16

The   subunit of adult hemoglobin has higher sequence similarity to __________ than to __________.

Accepted Answer

A)  fetal  Subunit; myoglobin 
B)  myoglobin; fetal  Subunit 
C)  adult  ubunit; fetal  Subunit 
D)  myoglobin; adult   subunit 
A)  fetal  Subunit; myoglobin 
B)  myoglobin; fetal  Subunit 
C)  adult  ubunit; fetal  Subunit 
D)  myoglobin; adult   subunit

Question 17

Both SERCA and the Na+-K+ ATPase contain a P domain.Explain the general function of this domain.

Accepted Answer

The P domain contains an amino

Question 18

Which of the following is NOT a symptom of anemia?

Accepted Answer

A)  fatigue 
B)  shortness of breath 
C)  cognitive decline 
D)  excessive bleeding 
A)  fatigue 
B)  shortness of breath 
C)  cognitive decline 
D)  excessive bleeding

Question 19

What type of transport is depicted in the figure below?

Accepted Answer

A)  membrane diffusion 
B)  passive transport of molecules down a concentration gradient 
C)  active transport of molecules down a concentration gradient 
D)  active transport of molecules up a concentration gradient 
A)  membrane diffusion 
B)  passive transport of molecules down a concentration gradient 
C)  active transport of molecules down a concentration gradient 
D)  active transport of molecules up a concentration gradient

Question 20

In studying a transporter,it is found that no external energy input is needed to facilitate the movement of a neutral compound through the transporter.The translocation rate of the compound reaches a plateau at high levels of compound.It is a(n)

Accepted Answer

A)  passive transport channel. 
B)  passive transport carrier. 
C)  active transport channel. 
D)  active transport carrier. 
A)  passive transport channel. 
B)  passive transport carrier. 
C)  active transport channel. 
D)  active transport carrier.

Exam 6: Protein Function

Consider the following interaction between a protein and its ligand: [P] + [L] [PL]. If [L] = 5 mM,[P] = 3 mM,and [PL] = 2 mM,what is the Ka for the interaction?

Consider the following interaction between a protein and its ligand: [P] + [L] [PL].If [L] = 5 mM,[P] = 3 mM,and [PL] = 2 mM,what is the dissociation constant?

Consider an oxygen binding curve for hemoglobin at pH 7.4.The binding curve would shift __________ if the pH is reduced to 7.2.

Based on the data shown below,the Kd for protein A is __________ the Kd for protein B.

Which of the following is NOT an ABC transporter?

Thin filaments __________,but thick filaments do/are not.

Explain the relationship between P50 of oxygen binding and Kd for oxygen binding to myoglobin.

Articulate the difference between a myoblast and a myofibril.

Which of the following is a positive effector for oxygen binding to hemoglobin?

Construct a complete actin-myosin reaction cycle by placing the following steps in order,starting with Ca2+ binding to troponin:

A proposed structure of a bacterial membrane protein shows that the distance between successive amino acids in a region crossing the membrane is 3.4 Å.Recall that a typical lipid bilayer is approximately 40 Å across.Which of the following is the most likely description of the protein?

An E.coli strain is discovered that has a mutation in the gene encoding RecBCD.The mutation causes RecBCD to not function as effectively as the wild-type RecBCD.Hypothesize how the growth rate of this strain would compare with that of the wild type.

Hemoglobin contains __________ -helices,__________ -sheets,and __________ globin folds.

Which of the following is true of sickle cell anemia?

Approximately how many amino acids are identical in both myoglobin and the adult -subunit of hemoglobin?

The subunit of adult hemoglobin has higher sequence similarity to __________ than to __________.

Both SERCA and the Na+-K+ ATPase contain a P domain.Explain the general function of this domain.

Which of the following is NOT a symptom of anemia?

What type of transport is depicted in the figure below?

In studying a transporter,it is found that no external energy input is needed to facilitate the movement of a neutral compound through the transporter.The translocation rate of the compound reaches a plateau at high levels of compound.It is a(n)

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

Based on the data shown below,the K_d for protein A is __________ the K_d for protein B.

Explain the relationship between P₅₀ of oxygen binding and K_d for oxygen binding to myoglobin.

Construct a complete actin-myosin reaction cycle by placing the following steps in order,starting with Ca²⁺ binding to troponin:

Hemoglobin contains -helices, -sheets,and __ globin folds.

The subunit of adult hemoglobin has higher sequence similarity to than to .

Both SERCA and the Na⁺-K⁺ ATPase contain a P domain.Explain the general function of this domain.