Question 1

Consider the following interaction between a protein and its ligand: [P] + [L]   [PL].Which equation describes the dissociation constant of this interaction?

Accepted Answer

A)  [PL] / [P][L] 
B)  [P][L] / [PL] 
C)  [PL] / [P] 
D)  [PL][P] / [L] 
A)  [PL] / [P][L] 
B)  [P][L] / [PL] 
C)  [PL] / [P] 
D)  [PL][P] / [L]

Question 2

Primary active ABC transporters and P-type transporters both require

Accepted Answer

A)  a conformational change to function. 
B)  two ATP binding half-sites. 
C)  solutes that move down their concentration gradients through the transporter. 
D)  removal of inhibitory subunits to function. 
A)  a conformational change to function. 
B)  two ATP binding half-sites. 
C)  solutes that move down their concentration gradients through the transporter. 
D)  removal of inhibitory subunits to function.

Question 3

There are two main structural differences between a typical   
-helix and the helix formed by gramicidin A.Describe the two structural differences and choose which structural characteristic is more important in the function of gramicidin A.Explain your choice.

Accepted Answer

A typical @#IMG-DLM&_TB6697_11 -helix is left hand

Question 4

Nuclear receptors are a type of

Accepted Answer

A)  metabolic enzyme. 
B)  structural protein. 
C)  transport protein. 
D)  cell signaling protein. 
A)  metabolic enzyme. 
B)  structural protein. 
C)  transport protein. 
D)  cell signaling protein.

Question 5

A protein that follows the concerted model of allosteric regulation would display a binding curve that is __________.A protein that follows the sequential model of allosteric regulation would display a binding curve that is __________.

Accepted Answer

A)  sigmoidal; hyperbolic 
B)  hyperbolic; sigmoidal 
C)  sigmoidal; sigmoidal 
D)  hyperbolic; hyperbolic 
A)  sigmoidal; hyperbolic 
B)  hyperbolic; sigmoidal 
C)  sigmoidal; sigmoidal 
D)  hyperbolic; hyperbolic

Question 6

Look at the data in the figure below.If the sequence for the   subunit was replaced with the  
Subunit sequence from a patient with sickle cell disease,the number of

Accepted Answer

A)  asterisks would decrease. 
B)  colons would increase. 
C)  periods would decrease. 
D)  asterisks would increase. 
A)  asterisks would decrease. 
B)  colons would increase. 
C)  periods would decrease. 
D)  asterisks would increase.

Question 7

Which of the following is NOT a transport protein?

Accepted Answer

A)  maltoporin 
B)  hemoglobin 
C)  Na+ channel 
D)  DNA polymerase 
A)  maltoporin 
B)  hemoglobin 
C)  Na+ channel 
D)  DNA polymerase

Question 8

Which of the following directly requires ATP to carry out its function?

Accepted Answer

A)  Cl-ion channel 
B)  malate dehydrogenase 
C)  myoglobin 
D)  protein kinase A 
A)  Cl-ion channel 
B)  malate dehydrogenase 
C)  myoglobin 
D)  protein kinase A

Question 9

A myoblast contain(s)__________,but a myofibril does not.

Accepted Answer

A)  actin 
B)  many nuclei 
C)  myosin 
D)  many sarcomeres 
A)  actin 
B)  many nuclei 
C)  myosin 
D)  many sarcomeres

Question 10

Passive membrane transporters CANNOT

Accepted Answer

A)  be small peptides. 
B)  contain  -sheets. 
C)  contain  -helices. 
D)  contain only hydrophobic amino acids. 
A)  be small peptides. 
B)  contain  -sheets. 
C)  contain  -helices. 
D)  contain only hydrophobic amino acids.

Question 11

Consider the movement of fructose into a cell by passive transport.If the extracellular concentration of fructose is 0.35 mM and the intracellular concentration of fructose is 0.42 mM,what is the free energy of transport in kJ/mol?

Accepted Answer

The answer of Consider the movement of fructose into a...

Question 12

The sarcomere length of isolated heart myoblasts can be measured over time in the laboratory.The myoblasts are maintained in a buffer during the experiment.In a control experiment,Ca2+ and ATP are added to the buffer and the change in sarcomere length is analyzed.If EDTA,a chelating agent that sequesters Ca2+,was included in the buffer,the length change would be __________ compared with a control experiment without EDTA.

Accepted Answer

A)  smaller 
B)  greater 
C)  the same 
D)  There is no way to determine the result given the information provided. 
A)  smaller 
B)  greater 
C)  the same 
D)  There is no way to determine the result given the information provided.

Question 13

Given the following concentrations,what is   
? [Mb] = 0.07,[MbO2] = 0.69,[O2] = 3.5 kPa

Accepted Answer

Question 14

The mutation that would lead to the most dramatic effect on Ca2+ transport is a mutation of

Accepted Answer

A)  Ser16 in phospholamban. 
B)  Thr17 in phospholamban. 
C)  Asp351 in SERCA. 
D)  Ser17 in E2P. 
A)  Ser16 in phospholamban. 
B)  Thr17 in phospholamban. 
C)  Asp351 in SERCA. 
D)  Ser17 in E2P.

Question 15

A reduction in the catalytic ability of carbonic anhydrase may lead to __________ efficient oxygen delivery by hemoglobin as a result of a(n)__________ Bohr effect.

Accepted Answer

A)  less; increased 
B)  more; increased 
C)  less; reduced 
D)  more; reduced 
A)  less; increased 
B)  more; increased 
C)  less; reduced 
D)  more; reduced

Question 16

Consider the transport scenario shown.Which curve represents the overall movement of the small molecule from the extracellular environment to the cytosol?

Accepted Answer

A)    
B)    
C)    
D)    
A)    
B)    
C)    
D)

Question 17

As 18 Na+ ions are exported out of the cell by the Na+-K+ ATPase membrane transport protein,__________ K+ ions are imported into the cell.

Accepted Answer

A)  2 
B)  12 
C)  18 
D)  24 
A)  2 
B)  12 
C)  18 
D)  24

Question 18

A new protein has been discovered that may be allosterically regulated.The protein is purified and analyzed by SDS-PAGE followed by staining with Coomassie blue.A single band at 42 kDa is seen on the gel.Explain how these data may or may not support the belief that the protein is an allosterically regulated protein.If they do support an allosterically regulated protein,propose the most likely model of allostery followed by the protein.

Accepted Answer

These data do not support the belief tha

Question 19

An effector binds to a single subunit of a multi-subunit allosteric protein.As a result,all subunits switch to the R state.This is an example of the __________ model of allostery.

Accepted Answer

A)  concerted 
B)  homotropic 
C)  sequential 
D)  combined 
A)  concerted 
B)  homotropic 
C)  sequential 
D)  combined

Question 20

Explain how a mutation in Asp351 of SERCA might alter Ca2+ transport.

Accepted Answer

Phosphorylation of Asp351 is r

Exam 6: Protein Function

Consider the following interaction between a protein and its ligand: [P] + [L] [PL].Which equation describes the dissociation constant of this interaction?

Primary active ABC transporters and P-type transporters both require

There are two main structural differences between a typical -helix and the helix formed by gramicidin A.Describe the two structural differences and choose which structural characteristic is more important in the function of gramicidin A.Explain your choice.

Nuclear receptors are a type of

A protein that follows the concerted model of allosteric regulation would display a binding curve that is __________.A protein that follows the sequential model of allosteric regulation would display a binding curve that is __________.

Look at the data in the figure below.If the sequence for the subunit was replaced with the Subunit sequence from a patient with sickle cell disease,the number of

Which of the following is NOT a transport protein?

Which of the following directly requires ATP to carry out its function?

A myoblast contain(s)__________,but a myofibril does not.

Passive membrane transporters CANNOT

Consider the movement of fructose into a cell by passive transport.If the extracellular concentration of fructose is 0.35 mM and the intracellular concentration of fructose is 0.42 mM,what is the free energy of transport in kJ/mol?

Given the following concentrations,what is ? [Mb] = 0.07,[MbO2] = 0.69,[O2] = 3.5 kPa

The mutation that would lead to the most dramatic effect on Ca2+ transport is a mutation of

A reduction in the catalytic ability of carbonic anhydrase may lead to __________ efficient oxygen delivery by hemoglobin as a result of a(n)__________ Bohr effect.

Consider the transport scenario shown.Which curve represents the overall movement of the small molecule from the extracellular environment to the cytosol?

As 18 Na+ ions are exported out of the cell by the Na+-K+ ATPase membrane transport protein,__________ K+ ions are imported into the cell.

An effector binds to a single subunit of a multi-subunit allosteric protein.As a result,all subunits switch to the R state.This is an example of the __________ model of allostery.

Explain how a mutation in Asp351 of SERCA might alter Ca2+ transport.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

A protein that follows the concerted model of allosteric regulation would display a binding curve that is .A protein that follows the sequential model of allosteric regulation would display a binding curve that is .

Given the following concentrations,what is ? [Mb] = 0.07,[MbO₂] = 0.69,[O₂] = 3.5 kPa

The mutation that would lead to the most dramatic effect on Ca²⁺ transport is a mutation of

A reduction in the catalytic ability of carbonic anhydrase may lead to efficient oxygen delivery by hemoglobin as a result of a(n) Bohr effect.

As 18 Na⁺ ions are exported out of the cell by the Na⁺-K⁺ ATPase membrane transport protein,__________ K⁺ ions are imported into the cell.

Explain how a mutation in Asp351 of SERCA might alter Ca²⁺ transport.