Question 1

The Swi-Snf complex affects chromatin by

Accepted Answer

A)  creating extra nucleosomes near the promoter. 
B)  being a promoter. 
C)  creating a nucleosome-free region near the promoter. 
D)  transcribing the chromatin. 
A)  creating extra nucleosomes near the promoter. 
B)  being a promoter. 
C)  creating a nucleosome-free region near the promoter. 
D)  transcribing the chromatin. C

Question 2

Bromodomain-containing complexes bind to __________ lysines in histones.

Accepted Answer

A)  methylated 
B)  acetylated 
C)  brominated 
D)  chlorinated 
A)  methylated 
B)  acetylated 
C)  brominated 
D)  chlorinated B

Question 3

Explain the expression of the Drosophila even-skipped gene.

Accepted Answer

Expression of the Drosophila even-skipped gene occurs in a series of seven stripes along the axis of the embryo,where each stripe has a separate set of regulatory controls.The eve stripe 2 enhancer region contains cis-acting sites for four different transcription factors that function together to restrict expression of the even structural gene to a small number of cells.

Question 4

The figure below shows __________ autoregulation and will __________.

Accepted Answer

A)  positive; reach a steady state 
B)  positive; be zero in the absence of an activator 
C)  negative; reach a steady state 
D)  negative; be zero in the absence of an activator 
A)  positive; reach a steady state 
B)  positive; be zero in the absence of an activator 
C)  negative; reach a steady state 
D)  negative; be zero in the absence of an activator

Question 5

The correct mRNA secondary structure of transcriptional termination is __________ stem-loop structure.

Accepted Answer

A)  2-3 
B)  3-4 
C)  1-3 
D)  2-4 
A)  2-3 
B)  3-4 
C)  1-3 
D)  2-4

Question 6

When the lysine side chain in a histone is acetylated,the amino group is now

Accepted Answer

A)  positively charged. 
B)  neutral. 
C)  negatively charged. 
D)  removed. 
A)  positively charged. 
B)  neutral. 
C)  negatively charged. 
D)  removed.

Question 7

A helix-turn-helix motif can best be described as a(n)__________ followed by a(n)__________.

Accepted Answer

A)    -helix;   -helix 
B)    -helix;   -helix 
C)    -helix;   -helix 
D)    -helix;   -helix 
A)    -helix;   -helix 
B)    -helix;   -helix 
C)    -helix;   -helix 
D)    -helix;   -helix

Question 8

IPTG causes the lac promoter to

Accepted Answer

A)  bind irreversibly to the DNA. 
B)  dissociate from the DNA. 
C)  perform ligand-regulated repression. 
D)  perform ligand-activated deregulation. 
A)  bind irreversibly to the DNA. 
B)  dissociate from the DNA. 
C)  perform ligand-regulated repression. 
D)  perform ligand-activated deregulation.

Question 9

Describe the three broad classes of nucleosome modifications.

Accepted Answer

(1)Histones in nucleosomes undergo a wid

Question 10

How is transcription factor recruitment used in eukaryotic gene regulatory circuits?

Accepted Answer

Recruitment refers to the property of tr

Question 11

Operons can be defined as units of DNA containing _________ gene(s)under control of __________ promotor(s).

Accepted Answer

A)  one; one 
B)  multiple; one 
C)  multiple; multiple 
D)  one; multiple 
A)  one; one 
B)  multiple; one 
C)  multiple; multiple 
D)  one; multiple

Question 12

The role of the CI protein in the lysogenic pathway is that the

Accepted Answer

A)  lysogenic pathway is favored when CI binds. 
B)  lytic pathway is favored when CI binds. 
C)  CI protein activates the PR promoter. 
D)  CI protein inhibits the PRM promoter. 
A)  lysogenic pathway is favored when CI binds. 
B)  lytic pathway is favored when CI binds. 
C)  CI protein activates the PR promoter. 
D)  CI protein inhibits the PRM promoter.

Question 13

Why are Arg,Lys,Gln,and Asn commonly found to make specific contact with nucleotide bases?

Accepted Answer

These amino acids are able to

Question 14

The eve stripe 2 enhancer can be defined as multiple __________ sites for at least four __________.

Accepted Answer

A)  trans-binding; transcription factor proteins 
B)  cis-binding; transcription factor proteins 
C)  cis-binding; insulator genes 
D)  cis-binding; gene promoters 
A)  trans-binding; transcription factor proteins 
B)  cis-binding; transcription factor proteins 
C)  cis-binding; insulator genes 
D)  cis-binding; gene promoters

Question 15

Compare negative and positive autoregulation.

Accepted Answer

In negative autoregulation a protein rep

Question 16

Differentiate between the lytic and lysogenic stages of bacteriophage $\lambda$ in terms of what genes are active.

Accepted Answer

Using Figure 23.31 for reference,it can

Question 17

List the chain of events that occurs after a pioneer factor binds to a cis-acting sequence.

Accepted Answer

As shown in Figure 23.47 from the text,p

Question 18

The proteins encoded by GAL1,GAL2,GAL7,and GAL10 are all needed for converting __________ to glucose-6-phosphate.

Accepted Answer

A)  glucose 
B)  fructose 
C)  Galactose 
D)  galactose 
A)  glucose 
B)  fructose 
C)  Galactose 
D)  galactose

Question 19

A reporter gene can be defined as a gene that

Accepted Answer

A)  has a product that is easy to detect. 
B)  prevents protein synthesis. 
C)  overproduces proteins. 
D)  causes an epigenetic sate. 
A)  has a product that is easy to detect. 
B)  prevents protein synthesis. 
C)  overproduces proteins. 
D)  causes an epigenetic sate.

Question 20

Why are long tracts of A residues not found in regions of stable nucleosome positioning?

Accepted Answer

A)  They are too flexible. 
B)  They are too stiff. 
C)  DNA cannot have long tracts of A residues. 
D)  DNA cannot have A residues. 
A)  They are too flexible. 
B)  They are too stiff. 
C)  DNA cannot have long tracts of A residues. 
D)  DNA cannot have A residues.

Exam 23: Gene Regulation

The Swi-Snf complex affects chromatin by

Bromodomain-containing complexes bind to __________ lysines in histones.

Explain the expression of the Drosophila even-skipped gene.

The figure below shows __________ autoregulation and will __________.

The correct mRNA secondary structure of transcriptional termination is __________ stem-loop structure.

When the lysine side chain in a histone is acetylated,the amino group is now

A helix-turn-helix motif can best be described as a(n)__________ followed by a(n)__________.

IPTG causes the lac promoter to

Describe the three broad classes of nucleosome modifications.

How is transcription factor recruitment used in eukaryotic gene regulatory circuits?

Operons can be defined as units of DNA containing _________ gene(s)under control of __________ promotor(s).

The role of the CI protein in the lysogenic pathway is that the

Why are Arg,Lys,Gln,and Asn commonly found to make specific contact with nucleotide bases?

The eve stripe 2 enhancer can be defined as multiple __________ sites for at least four __________.

Compare negative and positive autoregulation.

Differentiate between the lytic and lysogenic stages of bacteriophage λ\lambdaλ in terms of what genes are active.

List the chain of events that occurs after a pioneer factor binds to a cis-acting sequence.

The proteins encoded by GAL1,GAL2,GAL7,and GAL10 are all needed for converting __________ to glucose-6-phosphate.

A reporter gene can be defined as a gene that

Why are long tracts of A residues not found in regions of stable nucleosome positioning?

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Filters

The figure below shows autoregulation and will .

A helix-turn-helix motif can best be described as a(n) followed by a(n).

Operons can be defined as units of DNA containing _ gene(s)under control of __ promotor(s).

The eve stripe 2 enhancer can be defined as multiple sites for at least four .

Differentiate between the lytic and lysogenic stages of bacteriophage $\lambda$ in terms of what genes are active.