Question 1

When running an assay for luciferase,what reporter gene has been inserted into the plasmid?

Accepted Answer

A)  lacZ 
B)  luc 
C)  cat 
D)  gfp 
A)  lacZ 
B)  luc 
C)  cat 
D)  gfp

Question 2

What will happen to the lac operon when there are elevated levels of glucose in a cell?

Accepted Answer

A)  CRP binds strongly. 
B)  Transcription occurs at a very high rate of expression. 
C)  Transcription occurs at a very low rate of expression. 
D)  The lac repressor is bound. 
A)  CRP binds strongly. 
B)  Transcription occurs at a very high rate of expression. 
C)  Transcription occurs at a very low rate of expression. 
D)  The lac repressor is bound.

Question 3

The pioneer factor can be defined as the

Accepted Answer

A)  last transcription factor protein complex to bind. 
B)  first transcription factor protein complex to bind. 
C)  last cis-acting site in a chain. 
D)  first cis-acting site in a chain. 
A)  last transcription factor protein complex to bind. 
B)  first transcription factor protein complex to bind. 
C)  last cis-acting site in a chain. 
D)  first cis-acting site in a chain.

Question 4

Transcriptional elongation is favored by the trp operon when tryptophan levels are

Accepted Answer

A)  high. 
B)  low. 
C)  constant. 
D)  The trp operon is not affected by tryptophan levels. 
A)  high. 
B)  low. 
C)  constant. 
D)  The trp operon is not affected by tryptophan levels.

Question 5

If PRM is autoactivated and PR is inhibited,then __________ is/are bound.

Accepted Answer

A)  CI 
B)  Cro 
C)  both CI and Cro 
D)  neither CI and Cro 
A)  CI 
B)  Cro 
C)  both CI and Cro 
D)  neither CI and Cro

Question 6

A nucleosome can best be described as a protein core containing __________ molecules each of __________ histones and a segment of DNA.

Accepted Answer

A)  four; four 
B)  two; four 
C)  two; two 
D)  four; two 
A)  four; four 
B)  two; four 
C)  two; two 
D)  four; two

Question 7

When the Trp repressor is bound to the trp operon,it results in the __________ by RNA polymerase.

Accepted Answer

A)  inhibition of transcriptional initiation 
B)  disruption of transcriptional elongation 
C)  initiation 
D)  enhancement of transcriptional elongation 
A)  inhibition of transcriptional initiation 
B)  disruption of transcriptional elongation 
C)  initiation 
D)  enhancement of transcriptional elongation

Question 8

In cooperative DNA binding,the binding of

Accepted Answer

A)  both proteins must occur at the same time. 
B)  one protein molecule is more favored when another one is bound to a nearby site on the DNA. 
C)  one protein molecule is less favored when another one is bound to a nearby site on the DNA. 
D)  one protein molecule is only possible if two other molecules are bound to a nearby site on the DNA. 
A)  both proteins must occur at the same time. 
B)  one protein molecule is more favored when another one is bound to a nearby site on the DNA. 
C)  one protein molecule is less favored when another one is bound to a nearby site on the DNA. 
D)  one protein molecule is only possible if two other molecules are bound to a nearby site on the DNA.

Question 9

Pluripotent state can be defined as a __________ cell.

Accepted Answer

A)  differentiated 
B)  predifferentiated 
C)  G1 state of the 
D)  S state of the 
A)  differentiated 
B)  predifferentiated 
C)  G1 state of the 
D)  S state of the

Question 10

The phrase trans-acting factors is short for __________ factor protein.

Accepted Answer

A)  translation 
B)  transcription 
C)  transunion 
D)  transform 
A)  translation 
B)  transcription 
C)  transunion 
D)  transform

Question 11

How can the lac promoter and IPTG be used to produce large amounts of a specific protein?

Accepted Answer

As shown in Figure 23.16,the lac promote

Question 12

In negative autoregulation,

Accepted Answer

A)  proteins activate their own expression. 
B)  proteins repress their own expression. 
C)  a regulatory protein controls DNA expression. 
D)  a regulatory protein turns off DNA expression. 
A)  proteins activate their own expression. 
B)  proteins repress their own expression. 
C)  a regulatory protein controls DNA expression. 
D)  a regulatory protein turns off DNA expression.

Question 13

When histones are acetylated,the nucleosome-histone interaction

Accepted Answer

A)  is weakened. 
B)  is strengthened. 
C)  dissociates the nucleosome from the histone. 
D)  covalently binds the nucleosome to the histone. 
A)  is weakened. 
B)  is strengthened. 
C)  dissociates the nucleosome from the histone. 
D)  covalently binds the nucleosome to the histone.

Question 14

What is the function of the SOS regulatory system in the cell?

Accepted Answer

A)  DNA repair 
B)  DNA translation 
C)  lactose degradation 
D)  glucose inhibition 
A)  DNA repair 
B)  DNA translation 
C)  lactose degradation 
D)  glucose inhibition

Question 15

Stem cells can undergo two different pathways: cell __________ and cell __________.

Accepted Answer

A)  proliferation; dedifferentiation 
B)  proliferation; differentiation 
C)  differentiation; dedifferentiation 
D)  differentiation; integration 
A)  proliferation; dedifferentiation 
B)  proliferation; differentiation 
C)  differentiation; dedifferentiation 
D)  differentiation; integration

Question 16

What are the two key protein complexes recruited to the preinitiation complex by activator proteins?

Accepted Answer

A)  mediator complex and DNA polymerase 
B)  mediator complex and TFIID 
C)  TFIID and RNA polymerase 
D)  TFIID and DNA polymerase 
A)  mediator complex and DNA polymerase 
B)  mediator complex and TFIID 
C)  TFIID and RNA polymerase 
D)  TFIID and DNA polymerase

Question 17

What are the four transcription factors involved in the eve stripe 2 enhancer region?

Accepted Answer

Bicoid,Hun

Question 18

Introducing Oct4,Sox2,c-Myc,or Klf4 into a differentiated cell causes

Accepted Answer

A)  cell death. 
B)  a pluripotent state. 
C)  no change. 
D)  cell meiosis. 
A)  cell death. 
B)  a pluripotent state. 
C)  no change. 
D)  cell meiosis.

Question 19

What function do HAT and HDAC perform in the chromatin-modifying process?

Accepted Answer

A)  Both HAT and HDAC activate the gene. 
B)  Both HAT and HDAC repress the gene. 
C)  HAT represses the gene and HDAC activates the gene. 
D)  HAT activates the gene and HDAC represses the gene. 
A)  Both HAT and HDAC activate the gene. 
B)  Both HAT and HDAC repress the gene. 
C)  HAT represses the gene and HDAC activates the gene. 
D)  HAT activates the gene and HDAC represses the gene.

Question 20

A function of transcriptional activator proteins is to

Accepted Answer

A)  recruit gene promotors. 
B)  initiate RNA synthesis. 
C)  recruit chromatin modifiers. 
D)  initiate DNA synthesis. 
A)  recruit gene promotors. 
B)  initiate RNA synthesis. 
C)  recruit chromatin modifiers. 
D)  initiate DNA synthesis.

Exam 23: Gene Regulation

When running an assay for luciferase,what reporter gene has been inserted into the plasmid?

What will happen to the lac operon when there are elevated levels of glucose in a cell?

The pioneer factor can be defined as the

Transcriptional elongation is favored by the trp operon when tryptophan levels are

If PRM is autoactivated and PR is inhibited,then __________ is/are bound.

A nucleosome can best be described as a protein core containing __________ molecules each of __________ histones and a segment of DNA.

When the Trp repressor is bound to the trp operon,it results in the __________ by RNA polymerase.

In cooperative DNA binding,the binding of

Pluripotent state can be defined as a __________ cell.

The phrase trans-acting factors is short for __________ factor protein.

How can the lac promoter and IPTG be used to produce large amounts of a specific protein?

In negative autoregulation,

When histones are acetylated,the nucleosome-histone interaction

What is the function of the SOS regulatory system in the cell?

Stem cells can undergo two different pathways: cell __________ and cell __________.

What are the two key protein complexes recruited to the preinitiation complex by activator proteins?

What are the four transcription factors involved in the eve stripe 2 enhancer region?

Introducing Oct4,Sox2,c-Myc,or Klf4 into a differentiated cell causes

What function do HAT and HDAC perform in the chromatin-modifying process?

A function of transcriptional activator proteins is to

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Filters

If P_RM is autoactivated and P_R is inhibited,then __________ is/are bound.

A nucleosome can best be described as a protein core containing molecules each of histones and a segment of DNA.

Stem cells can undergo two different pathways: cell and cell .