Question 1

Matching
-Different enzymes that catalyze the same reaction, although may be found in different tissues, are known as ______.

Accepted Answer

A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B] 
A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B]

Question 2

Matching
-Assume a first order reaction, the rate of the reaction 2A$\rarr$B is dependent on ______.

Accepted Answer

A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B] 
A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B]

Question 3

Enzyme activity in cells is controlled by which of the following?
I.covalent modifications
II.modulation of expression levels
III.feedback inhibition
IV.allosteric effectors

Accepted Answer

A) I 
B) II 
C) III 
D) III, IV 
E) I, II, III, IV 
A) I 
B) II 
C) III 
D) III, IV 
E) I, II, III, IV

Question 4

The following questions refer to the overall transformation shown in the following reaction:  
-For the reaction, the steady state assumption

Accepted Answer

A) implies that k1=k−1 
B) implies that k−1 and k2 are such that the [ES] = k1[ES] 
C) [P]>>[E] 
D) [S] = [P] 
E) ES breakdown occurs at the same rate as ES formation 
A) implies that k1=k−1 
B) implies that k−1 and k2 are such that the [ES] = k1[ES] 
C) [P]>>[E] 
D) [S] = [P] 
E) ES breakdown occurs at the same rate as ES formation

Question 5

I propose to design a new drug which will act as an inhibitor for an enzyme.If I have used all current information about the mechanism of this enzyme to design this inhibitor and I carefully engineer it with similar chemical properties of the transition state, what type of inhibitor am I attempting to engineer and how will I know if I have succeeded?

Accepted Answer

A) A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax. 
B) A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM. 
C) A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM. 
D) A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax. 
E) None of the above. 
A) A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax. 
B) A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM. 
C) A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM. 
D) A uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax. 
E) None of the above.

Question 6

The following questions refer to the overall transformation shown in the following reaction:  
-Which of the following is (are)true?

Accepted Answer

A) The [ES] will remain constant if k2>k1 and k−12. 
B) The reaction is zero order with respect to [S] if [S]>>[E] 
C) It describes a double displacement reaction 
D) All of the above are true. 
E) None of the above is true. 
A) The [ES] will remain constant if k2>k1 and k−12. 
B) The reaction is zero order with respect to [S] if [S]>>[E] 
C) It describes a double displacement reaction 
D) All of the above are true. 
E) None of the above is true.

Question 7

Enzyme E is responsible for conversion of substrate X to product U.As a result of this conversion electrons are transported to a coenzyme (FAD)within Enzyme E.In order for the reaction to be completed, a second substrate NAD+ must also bind Enzyme E and collect stored electrons (which converts it to product, NADH).The graph below shows the data while varying X, with fixed concentrations of NAD+.What type of multi-substrate mechanism does enzyme E utilize?

Accepted Answer

A) sequential - Ordered 
B) sequential - Random 
C) simultaneous addition 
D) Ping Pong 
E) Sequential but the data cannot differentiate between ordered and random. 
A) sequential - Ordered 
B) sequential - Random 
C) simultaneous addition 
D) Ping Pong 
E) Sequential but the data cannot differentiate between ordered and random.

Question 8

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the KM?

Accepted Answer

A) 0.24 μM/s 
B) 18 μM 
C) 0.2 μM 
D) 0.24 μM 
E) 0.12 μM/s 
A) 0.24 μM/s 
B) 18 μM 
C) 0.2 μM 
D) 0.24 μM 
E) 0.12 μM/s

Question 9

KM is

Accepted Answer

A) a measure of the catalytic efficiency of the enzyme. 
B) equal to half of Vmax 
C) the rate constant for the reaction ES $\rightarrow$ E + P. 
D) the [S] that half-saturates the enzyme. 
E) a ratio of substrate concentration relative to catalytic power. 
A) a measure of the catalytic efficiency of the enzyme. 
B) equal to half of Vmax 
C) the rate constant for the reaction ES $\rightarrow$ E + P. 
D) the [S] that half-saturates the enzyme. 
E) a ratio of substrate concentration relative to catalytic power.

Question 10

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO).What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s−1.)

Accepted Answer

A) 0.050 M 
B) 0.014 M 
C) 0.018 M 
D) 1.2 M 
E) 0.025 M 
A) 0.050 M 
B) 0.014 M 
C) 0.018 M 
D) 1.2 M 
E) 0.025 M

Question 11

When [S] = KM, $\upsilon$0 = (_____)× (Vmax).

Accepted Answer

A) [S] 
B) 0.75 
C) 0.5 
D) KM 
E) kcat 
A) [S] 
B) 0.75 
C) 0.5 
D) KM 
E) kcat

Question 12

Matching
-A lead compound for a new drug should bind to its target protein with a very ______.

Accepted Answer

A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B] 
A) isozymes 
B) [A] 
C) the rate constant 
D) Ping Pong 
E) bimolecular
F)ES complex
G)random ordered
H)competitive inhibition
I)unimolecular
J)[A]²
K)Competitive inhibition
L)phosphorylation
M)small KS
N)large KS
O)uncompetitive inhibition
P)[B]

Question 13

A Lineweaver-Burk plot is also referred to as
I.a sigmoidal plot.
II.a linear plot.
III.a Michaelis-Menten plot.
IV.a double reciprocal plot.

Accepted Answer

A) II 
B) II, III 
C) IV 
D) II, IV 
E) III, IV 
A) II 
B) II, III 
C) IV 
D) II, IV 
E) III, IV

Question 14

Learning objective: Reaction Kinetics
The following questions refer to the diagram (with boxes where it has been left incomplete):  
-Which of the following is correct in regards to the diagram above?

Accepted Answer

A) X=A, Y=B, Z=P 
B) X=B, Y=A, Z=Q 
C) X=E, Y=A, Z=E 
D) X=E, Y=B, Z=Q 
E) X=E, Y=B, Z=P 
A) X=A, Y=B, Z=P 
B) X=B, Y=A, Z=Q 
C) X=E, Y=A, Z=E 
D) X=E, Y=B, Z=Q 
E) X=E, Y=B, Z=P

Question 15

________ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _____ test.

Accepted Answer

A) Phase 1; single blind 
B) Phase 1; double blind 
C) Phase 2; single blind 
D) Phase 2; double blind 
E) Phase 3; double blind 
A) Phase 1; single blind 
B) Phase 1; double blind 
C) Phase 2; single blind 
D) Phase 2; double blind 
E) Phase 3; double blind

Question 16

An enzyme is near maximum efficiency when

Accepted Answer

A) its turnover number is near Vmax. 
B) kcat/KM is near 108 M-1s-1. 
C) k1 -1. 
D) kcat/KM is equal to kcat. 
E) KM is large when k2 exceeds k1. 
A) its turnover number is near Vmax. 
B) kcat/KM is near 108 M-1s-1. 
C) k1 -1. 
D) kcat/KM is equal to kcat. 
E) KM is large when k2 exceeds k1.

Question 17

Pseudo-first-order reaction kinetics would be observed for the reaction A + B $\rarr$ C

Accepted Answer

A) if [A] or [B] > [C]. 
B) if [C]>[A] and [C]>[B]. 
C) if [A] or [B] = 0. 
D) if [C] = 0. 
E) none of the above 
A) if [A] or [B] > [C]. 
B) if [C]>[A] and [C]>[B]. 
C) if [A] or [B] = 0. 
D) if [C] = 0. 
E) none of the above

Question 18

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the Vmax?

Accepted Answer

A) 0.24 μM/s 
B) 18 μM 
C) 0.2 μM 
D) 0.24 μM 
E) 0.12 μM/s 
A) 0.24 μM/s 
B) 18 μM 
C) 0.2 μM 
D) 0.24 μM 
E) 0.12 μM/s

Question 19

Determine the KM and Vmax from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)

Accepted Answer

A) KM = [0.006]; Vmax = 0.0075/s 
B) KM = [0.196]; Vmax = 0.0075/s 
C) KM = [165]; Vmax = 33/s 
D) KM = [33]; Vmax = 167/s 
E) KM = [270]; Vmax x = 68/s 
A) KM = [0.006]; Vmax = 0.0075/s 
B) KM = [0.196]; Vmax = 0.0075/s 
C) KM = [165]; Vmax = 33/s 
D) KM = [33]; Vmax = 167/s 
E) KM = [270]; Vmax x = 68/s

Question 20

Learning objective: Reaction Kinetics
The following questions refer to the diagram (with boxes where it has been left incomplete):  
-This diagram refers to a (an)

Accepted Answer

A) Ping Pong reaction. 
B) ordered bisubstrate reaction. 
C) random bisubstrate reaction. 
D) double order ping pong reaction 
E) X, Y, and Z must be provided in order to answer correctly 
A) Ping Pong reaction. 
B) ordered bisubstrate reaction. 
C) random bisubstrate reaction. 
D) double order ping pong reaction 
E) X, Y, and Z must be provided in order to answer correctly

Exam 12: Enzyme Kinetics, Inhibition and Control

Matching -Different enzymes that catalyze the same reaction, although may be found in different tissues, are known as ______.

Matching -Assume a first order reaction, the rate of the reaction 2A →\rarr→ B is dependent on ______.

Enzyme activity in cells is controlled by which of the following? I.covalent modifications II.modulation of expression levels III.feedback inhibition IV.allosteric effectors

The following questions refer to the overall transformation shown in the following reaction: -For the reaction, the steady state assumption

The following questions refer to the overall transformation shown in the following reaction: -Which of the following is (are)true?

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the KM?

KM is

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO).What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s−1.)

When [S] = KM, υ\upsilonυ 0 = (_____)× (Vmax).

Matching -A lead compound for a new drug should bind to its target protein with a very ______.

A Lineweaver-Burk plot is also referred to as I.a sigmoidal plot. II.a linear plot. III.a Michaelis-Menten plot. IV.a double reciprocal plot.

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete): -Which of the following is correct in regards to the diagram above?

________ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _____ test.

An enzyme is near maximum efficiency when

Pseudo-first-order reaction kinetics would be observed for the reaction A + B →\rarr→ C

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the Vmax?

Determine the KM and Vmax from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)

Learning objective: Reaction Kinetics The following questions refer to the diagram (with boxes where it has been left incomplete): -This diagram refers to a (an)

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Primary Structure

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters

Matching -Assume a first order reaction, the rate of the reaction 2A $\rarr$ B is dependent on ______.

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the K_M?

K_M is

The breakdown of dopamine is catalyzed by the enzyme monoamine oxidase (MAO).What is the final concentration of product if the starting dopamine concentration is 0.050 M and the reaction runs for 5 seconds.(Assume the rate constant for the reaction is 0.249 s⁻¹.)

When [S] = K_M, $\upsilon$ ₀= (_____)× (V_max).

____ clinical trials are focused on evaluating the efficacy of new drug candidates, and usually use _ test.

Pseudo-first-order reaction kinetics would be observed for the reaction A + B $\rarr$ C

From the graph below plotting data that was collected under steady state conditions, velocity on the y-axis in units of μM/s and substrate concentration of the x-axis in units of μM, what is the V_max?

Determine the K_M and V_max from the following graph.(Note: On the x-axis the minor tick mark spacing is 0.005; on the y-axis the minor tick mark spacing is 0.002)