Question 1

Which statement about insulin is correct?

Accepted Answer

A) Insulin is composed of two polypeptides, the A chain and the B chain. 
B) Insulin contains an intrachain disulfide bond. 
C) Insulin contains interchain disulfide bonds. 
D) The A chain and the B chain of insulin are encoded by a single gene. 
E) All of the above are correct. 
A) Insulin is composed of two polypeptides, the A chain and the B chain. 
B) Insulin contains an intrachain disulfide bond. 
C) Insulin contains interchain disulfide bonds. 
D) The A chain and the B chain of insulin are encoded by a single gene. 
E) All of the above are correct.

Question 2

Which physical characteristic is not commonly used in protein separation?

Accepted Answer

A) solubility 
B) stereochemistry 
C) size 
D) charge 
E) polarity 
A) solubility 
B) stereochemistry 
C) size 
D) charge 
E) polarity

Question 3

Protein sequences are customarily 'reconstructed' from sequenced fragments because

Accepted Answer

A) protein purification invariably results in the fragmentation of the protein of interest. 
B) proteins are naturally and inevitably cleaved by proteolytic enzymes. 
C) proteins are composed of multiple subunits. 
D) large polypeptides cannot be directly sequenced. 
E) all of the above 
A) protein purification invariably results in the fragmentation of the protein of interest. 
B) proteins are naturally and inevitably cleaved by proteolytic enzymes. 
C) proteins are composed of multiple subunits. 
D) large polypeptides cannot be directly sequenced. 
E) all of the above

Question 4

Five graduate students prepare extracts from 5 different tissues.Each student measures the total amount of alcohol dehydrogenase and the total amount of protein in his or her extract.Which extract has the highest specific activity?

Accepted Answer

The answer of Five graduate students prepare extracts from 5...

Question 5

Matching
-In SDS-PAGE, disulfide-linked polypeptides can be separated after reacting the protein first with ______.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 6

Matching
-The endoprotease ______ cleaves polypeptides on the C-terminal side of certain bulky hydrophobic amino acid residues.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 7

Paralogous genes are

Accepted Answer

A) genes that do not encode protein. 
B) genes of slowly evolving proteins. 
C) relics of genes that are not expressed. 
D) genes of rapidly evolving proteins. 
E) the results of gene duplication. 
A) genes that do not encode protein. 
B) genes of slowly evolving proteins. 
C) relics of genes that are not expressed. 
D) genes of rapidly evolving proteins. 
E) the results of gene duplication.

Question 8

Which of the following 'assays' would be most specific for a particular protein?

Accepted Answer

A) Bradford assay 
B) UV absorptivity 
C) radioimmunoassay 
D) molar absorptivity 
E) amino acid analysis 
A) Bradford assay 
B) UV absorptivity 
C) radioimmunoassay 
D) molar absorptivity 
E) amino acid analysis

Which statement about insulin is correct?

Which physical characteristic is not commonly used in protein separation?

Protein sequences are customarily 'reconstructed' from sequenced fragments because

Five graduate students prepare extracts from 5 different tissues.Each student measures the total amount of alcohol dehydrogenase and the total amount of protein in his or her extract.Which extract has the highest specific activity?

Matching -In SDS-PAGE, disulfide-linked polypeptides can be separated after reacting the protein first with ______.

Matching -The endoprotease ______ cleaves polypeptides on the C-terminal side of certain bulky hydrophobic amino acid residues.

Paralogous genes are

Which of the following 'assays' would be most specific for a particular protein?

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters

Exam 5: Proteins: Primary Structure

Which statement about insulin is correct?

Which physical characteristic is not commonly used in protein separation?

Protein sequences are customarily 'reconstructed' from sequenced fragments because

Five graduate students prepare extracts from 5 different tissues.Each student measures the total amount of alcohol dehydrogenase and the total amount of protein in his or her extract.Which extract has the highest specific activity?

Matching -In SDS-PAGE, disulfide-linked polypeptides can be separated after reacting the protein first with ______.

Matching -The endoprotease ______ cleaves polypeptides on the C-terminal side of certain bulky hydrophobic amino acid residues.

Paralogous genes are

Which of the following 'assays' would be most specific for a particular protein?

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Transcription and Rna Processing

Translation

Regulation of Gene Expression

Filters