Question 1

How are units for exchange transfusion prepared?

Accepted Answer

A)  Group O red blood cells and group O plasma 
B)  Group O red blood cells and group A plasma 
C)  Group O red blood cells and group AB plasma 
D)  Group O red blood cells and group B plasma 
A)  Group O red blood cells and group O plasma 
B)  Group O red blood cells and group A plasma 
C)  Group O red blood cells and group AB plasma 
D)  Group O red blood cells and group B plasma

Question 2

How is an intrauterine transfusion performed?

Accepted Answer

A)  RBCs are injected into the placenta. 
B)  RBCs are injected into the fetal peritoneal cavity. 
C)  RBCs are injected into the maternal peritoneal cavity. 
D)  RBCs are injected into the mother and diffused through the placenta to the fetus. 
A)  RBCs are injected into the placenta. 
B)  RBCs are injected into the fetal peritoneal cavity. 
C)  RBCs are injected into the maternal peritoneal cavity. 
D)  RBCs are injected into the mother and diffused through the placenta to the fetus.

Question 3

Which of the following reagents can be used to determine the immunoglobulin class of anti-M?

Accepted Answer

A)  PEG 
B)  Chloroquine 
C)  2-mercaptoethanol 
D)  Ficin 
A)  PEG 
B)  Chloroquine 
C)  2-mercaptoethanol 
D)  Ficin

Question 4

An O-positive mother gave birth to an A- negative baby. After 24 hours the newborn's bilirubin level rose to 18 mg/dL. A DAT performed on the cord blood specimen was positive with polyspecific AHG and anti-IgG reagents. It is probable that from maternal circulation is coating the newborn's red blood cells.

Accepted Answer

A)  anti-A, B 
B)  anti-B 
C)  anti-A 
D)  anti-D 
A)  anti-A, B 
B)  anti-B 
C)  anti-A 
D)  anti-D

Question 5

All of the following are true regarding antibody titration of maternal IgG antibodies except:

Accepted Answer

A)  the method must include the indirect antiglobulin test. 
B)  the difference of two or more dilutions between titrations is considered significant. 
C)  red blood cells should consist of the same genotype for each titration. 
D)  the first serum specimen should be run in parallel with all subsequent titrations. 
A)  the method must include the indirect antiglobulin test. 
B)  the difference of two or more dilutions between titrations is considered significant. 
C)  red blood cells should consist of the same genotype for each titration. 
D)  the first serum specimen should be run in parallel with all subsequent titrations.

Question 6

A cord blood specimen from a jaundiced infant should be tested for which of the following?

Accepted Answer

A)  ABO 
B)  Rh 
C)  DAT 
D)  All of the above 
A)  ABO 
B)  Rh 
C)  DAT 
D)  All of the above

Question 7

A 300-µg dose of Rh-immune globulin contains sufficient anti-D to protect against how much whole blood?

Accepted Answer

A)  15 mL 
B)  30 mL 
C)  50 mL 
D)  100 mL 
A)  15 mL 
B)  30 mL 
C)  50 mL 
D)  100 mL

Question 8

What tests are indicated for cord blood specimens if the mother has made anti-K?

Accepted Answer

A)  ABO, Rh, antibody screen 
B)  ABO, Rh, DAT 
C)  ABO, Rh, cold autoabsorption 
D)  Kleihauer-Betke 
A)  ABO, Rh, antibody screen 
B)  ABO, Rh, DAT 
C)  ABO, Rh, cold autoabsorption 
D)  Kleihauer-Betke

Question 9

The D-positive fetal cells in Rh-HDN are .

Accepted Answer

A)  homozygous 
B)  heterozygous 
C)  amorphic 
D)  homologous 
A)  homozygous 
B)  heterozygous 
C)  amorphic 
D)  homologous

Question 10

All of the following are characteristic of ABO hemolytic disease of the fetus and newborn (HDFN) except:

Accepted Answer

A)  the mother is group O. 
B)  the mother has anti-A,B. 
C)  the antibody is IgM. 
D)  the infant has mild HDFN. 
A)  the mother is group O. 
B)  the mother has anti-A,B. 
C)  the antibody is IgM. 
D)  the infant has mild HDFN.

Question 11

Which of the following red blood cells is appropriate for neonatal transfusions?

Accepted Answer

A)  Group A, CMV-negative 
B)  Group AB, CMV-negative 
C)  Group O, CMV+ 
D)  Group O, CMV-negative 
A)  Group A, CMV-negative 
B)  Group AB, CMV-negative 
C)  Group O, CMV+ 
D)  Group O, CMV-negative

Question 12

In order for the mother to be considered for Rh-immune globulin, her Rh type must be , and her newborn must be .

Accepted Answer

A)  Du-positive/Rh-positive 
B)  Rh-negative/Rh-positive 
C)  Du-negative/Du-negative 
D)  Rh-positive/Rh-negative 
A)  Du-positive/Rh-positive 
B)  Rh-negative/Rh-positive 
C)  Du-negative/Du-negative 
D)  Rh-positive/Rh-negative

Question 13

All Rh-negative recipients who are transfused with as little as 1 mL of Rh-positive cells will develop anti-D.

Accepted Answer

A) True 
 B)False

Question 14

Active immunization induced by Rh(D) antigen can be prevented by the concurrent administration of:

Accepted Answer

A)  gamma globulin. 
B)  Rh immunoglobulin. 
C)  alpha-1 protease inhibitor. 
D)  immune serum globulin. 
A)  gamma globulin. 
B)  Rh immunoglobulin. 
C)  alpha-1 protease inhibitor. 
D)  immune serum globulin.

Question 15

Why is reverse grouping omitted in neonate ABO grouping?

Accepted Answer

A)  Maternal ABO antibody is identical to newborn ABO antibody. 
B)  Maternal antibodies mask the ABO antibodies of the neonate. 
C)  Newborns do not produce isoagglutinins of their own. 
D)  None of the above 
A)  Maternal ABO antibody is identical to newborn ABO antibody. 
B)  Maternal antibodies mask the ABO antibodies of the neonate. 
C)  Newborns do not produce isoagglutinins of their own. 
D)  None of the above

Question 16

The most important serologic test for the diagnosis of HDFN is the with anti-IgG reagents.

Accepted Answer

A)  elution 
B)  IAT 
C)  DAT 
D)  fetal screen 
A)  elution 
B)  IAT 
C)  DAT 
D)  fetal screen

Question 17

Rh-immune globulin should be given within how many hours after delivery?

Accepted Answer

A)  24 
B)  48 
C)  36 
D)  72 
A)  24 
B)  48 
C)  36 
D)  72

Question 18

Why is the immediate spin eliminated in the prenatal antibody screen?

Accepted Answer

A)  To reduce the detection of autoantibodies 
B)  To reduce the detection of IgG antibodies 
C)  To reduce the detection of IgM antibodies 
D)  None of the above 
A)  To reduce the detection of autoantibodies 
B)  To reduce the detection of IgG antibodies 
C)  To reduce the detection of IgM antibodies 
D)  None of the above

Question 19

In the event of a clinically significant antibody found in the mother's serum, which of the following must be performed to determine its concentration?

Accepted Answer

A)  Fetal screen 
B)  Antibody screen 
C)  Antibody titer 
D)  Elution 
A)  Fetal screen 
B)  Antibody screen 
C)  Antibody titer 
D)  Elution

Question 20

Why is the Rh-positive firstborn of an Rh-negative mother unaffected by Rh hemolytic disease of the fetus and newborn (Rh HDFN)?

Accepted Answer

A)  The plasma volume of the mother is tripled during the first pregnancy which dilutes anti-D. 
B)  The titer of anti-D is too low to cause destruction of fetal antigens. 
C)  The mother has not been immunized to the D antigen before placental separation. 
D)  None of the above 
A)  The plasma volume of the mother is tripled during the first pregnancy which dilutes anti-D. 
B)  The titer of anti-D is too low to cause destruction of fetal antigens. 
C)  The mother has not been immunized to the D antigen before placental separation. 
D)  None of the above

Exam 20: Hemolytic Disease of the Fetus and Newborn

How are units for exchange transfusion prepared?

How is an intrauterine transfusion performed?

Which of the following reagents can be used to determine the immunoglobulin class of anti-M?

All of the following are true regarding antibody titration of maternal IgG antibodies except:

A cord blood specimen from a jaundiced infant should be tested for which of the following?

A 300-µg dose of Rh-immune globulin contains sufficient anti-D to protect against how much whole blood?

What tests are indicated for cord blood specimens if the mother has made anti-K?

The D-positive fetal cells in Rh-HDN are .

All of the following are characteristic of ABO hemolytic disease of the fetus and newborn (HDFN) except:

Which of the following red blood cells is appropriate for neonatal transfusions?

In order for the mother to be considered for Rh-immune globulin, her Rh type must be , and her newborn must be .

All Rh-negative recipients who are transfused with as little as 1 mL of Rh-positive cells will develop anti-D.

Active immunization induced by Rh(D) antigen can be prevented by the concurrent administration of:

Why is reverse grouping omitted in neonate ABO grouping?

The most important serologic test for the diagnosis of HDFN is the with anti-IgG reagents.

Rh-immune globulin should be given within how many hours after delivery?

Why is the immediate spin eliminated in the prenatal antibody screen?

In the event of a clinically significant antibody found in the mother's serum, which of the following must be performed to determine its concentration?

Why is the Rh-positive firstborn of an Rh-negative mother unaffected by Rh hemolytic disease of the fetus and newborn (Rh HDFN)?

Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends

Basic Genetics

Fundamentals of Immunology

Concepts in Molecular Biology

The Antiglobulin Test

The Abo Blood Group System

The Rh Blood Group System

Blood Group Terminology and Common Blood Groups

Uncommon Blood Groups

Detection and Identification of Antibodies

Pre-Transfusion Testing

Blood Bank Technologies and Automation

Donor Selection

Transfusion-Transmitted Diseases

Component Preparation

Transfusion Therapy

Adverse Effects of Blood Transfusion

Apheresis

Cellular Therapy in the Transplant Setting

Autoimmune Hemolytic Anemias

Tissue Banking As Part of the Transfusion Service

The Hla System

Relationship Testing

Quality Management

Patient Blood Management

Transfusion Safety and Federal Regulatory Requirements

Laboratory Information Systems in the Blood Bank

Medicolegal and Ethical Aspects of Providing Blood Collection and Transfusion Services

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