Question 1

In warm autoimmune hemolytic anemia, the autoantibody will frequently demonstrate -like specificity.

Accepted Answer

A)  Fya 
B)  K 
C)  Rh 
D)  Jkb 
A)  Fya 
B)  K 
C)  Rh 
D)  Jkb

Question 2

What is the most frequent antibody specificity in CHD?

Accepted Answer

A)  Anti-IH 
B)  Anti-i 
C)  Anti-I 
D)  Anti-P 
A)  Anti-IH 
B)  Anti-i 
C)  Anti-I 
D)  Anti-P

Question 3

Anti-K was identified in absorbed serum of a patient with warm autoimmune hemolytic anemia. If a red blood cell phenotype was performed using an indirect antiglobulin test to ensure the patient was negative for K antigen, what would the technologist find?

Accepted Answer

A)  A negative result after cells were treated with ficin 
B)  A positive result due to IgM coating cells 
C)  Patient would type negative for K antigen 
D)  A positive result due to IgG coating cells 
A)  A negative result after cells were treated with ficin 
B)  A positive result due to IgM coating cells 
C)  Patient would type negative for K antigen 
D)  A positive result due to IgG coating cells

Question 4

A 28-year-old female with cold hemagglutinin disease has a positive DAT. When the DAT is repeated using monospecific reagent, which of the following is most likely to be detected?

Accepted Answer

A)  IgM 
B)  IgG 
C)  C3d 
D)  C4d 
A)  IgM 
B)  IgG 
C)  C3d 
D)  C4d

Question 5

How can persons with CHD avoid hemolytic episodes?

Accepted Answer

A)  Have a splenectomy 
B)  Move to a warm climate 
C)  Corticosteroid therapy 
D)  None of the above 
A)  Have a splenectomy 
B)  Move to a warm climate 
C)  Corticosteroid therapy 
D)  None of the above

Question 6

In the case of an AB-positive individual, what test must be performed to ensure that a warm-reacting autoantibody is not causing false-positive reactions?

Accepted Answer

A)  Rh control 
B)  DAT 
C)  IAT 
D)  Rosette test 
A)  Rh control 
B)  DAT 
C)  IAT 
D)  Rosette test

Question 7

Which of the following is a characteristic of autoantibodies?

Accepted Answer

A)  The DAT is only reactive with anti-C3d. 
B)  Antibody consists of IgM immunoglobulin. 
C)  Antibody reacts with low-incidence antigens. 
D)  Antibody reacts with high-incidence antigens. 
A)  The DAT is only reactive with anti-C3d. 
B)  Antibody consists of IgM immunoglobulin. 
C)  Antibody reacts with low-incidence antigens. 
D)  Antibody reacts with high-incidence antigens.

Question 8

What is the primary goal for treatment in patients with warm autoimmune hemolytic anemia?

Accepted Answer

A)  Reduce neoplastic tumors 
B)  Treat coexisting anemia 
C)  Treat the underlying disease 
D)  None of the above 
A)  Reduce neoplastic tumors 
B)  Treat coexisting anemia 
C)  Treat the underlying disease 
D)  None of the above

Question 9

Which of the following can be found in warm autoimmune hemolytic anemia in the presence of hypoplastic marrow?

Accepted Answer

A)  Reticulocytopenia 
B)  Polychromasia 
C)  Reticulocytosis 
D)  Thrombocytopenia 
A)  Reticulocytopenia 
B)  Polychromasia 
C)  Reticulocytosis 
D)  Thrombocytopenia

Question 10

Which alloantibody is frequently present in the serum of i adults?

Accepted Answer

A)  Anti-IH 
B)  Anti-H 
C)  Anti-I 
D)  All of the above 
A)  Anti-IH 
B)  Anti-H 
C)  Anti-I 
D)  All of the above

Question 11

Which of the following regarding the immune complex mechanism in serologic testing is true?

Accepted Answer

A)  The antibody is directed against a red blood cell antigen. 
B)  The antibody screen will be positive in Coombs' phase. 
C)  The DAT will be positive with monospecific C3 but negative with IgG. 
D)  The eluate will be positive. 
A)  The antibody is directed against a red blood cell antigen. 
B)  The antibody screen will be positive in Coombs' phase. 
C)  The DAT will be positive with monospecific C3 but negative with IgG. 
D)  The eluate will be positive.

Question 12

Which of the following describes a cold autologous absorption procedure?

Accepted Answer

A)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 37°C; autoantibody is removed and alloantibody remains in the serum. 
B)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 37°C; alloantibody is removed while autoantibody remains in the serum. 
C)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 4°C; alloantibody is removed while autoantibody remains in the serum. 
D)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 4°C; autoantibody is removed while alloantibody remains in the serum. 
A)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 37°C; autoantibody is removed and alloantibody remains in the serum. 
B)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 37°C; alloantibody is removed while autoantibody remains in the serum. 
C)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 4°C; alloantibody is removed while autoantibody remains in the serum. 
D)  An aliquot of patient cells is incubated with an equal aliquot of patient serum at 4°C; autoantibody is removed while alloantibody remains in the serum.

Question 13

Approximately what percentage of warm autoimmune hemolytic anemia will produce a positive DAT with both IgG and C3d antibodies?

Accepted Answer

A)  20% 
B)  67% 
C)  10% 
D)  13% 
A)  20% 
B)  67% 
C)  10% 
D)  13%

Question 14

Which of the following is a characteristic red blood cell morphology seen on a peripheral blood smear from a patient with warm autoimmune hemolytic anemia?

Accepted Answer

A)  Stomatocytes 
B)  Teardrop cells 
C)  Spherocytes 
D)  Target cells 
A)  Stomatocytes 
B)  Teardrop cells 
C)  Spherocytes 
D)  Target cells

Question 15

The onset of warm autoimmune hemolytic anemia (WAIHA) may be precipitated by:

Accepted Answer

A)  pregnancy. 
B)  bacterial infection. 
C)  trauma. 
D)  all of the above. 
A)  pregnancy. 
B)  bacterial infection. 
C)  trauma. 
D)  all of the above.

Question 16

In the digitonin-acid elution procedure, what is the purpose of adding phosphate buffer to the eluate solution?

Accepted Answer

A)  To disperse any debris left in the eluate 
B)  To protect against hemolysis 
C)  To restore neutrality to the eluate 
D)  To elute antibody from red blood cell membrane 
A)  To disperse any debris left in the eluate 
B)  To protect against hemolysis 
C)  To restore neutrality to the eluate 
D)  To elute antibody from red blood cell membrane

Question 17

How might a technologist detect a patient with drug-induced hemolytic anemia?

Accepted Answer

A)  Positive rosette test 
B)  Positive antibody screen 
C)  Positive DAT 
D)  ABO discrepancy 
A)  Positive rosette test 
B)  Positive antibody screen 
C)  Positive DAT 
D)  ABO discrepancy

Question 18

All of the following are characteristics of benign cold autoagglutinins except:

Accepted Answer

A)  Antibodies react best with ficin-treated cells. 
B)  Autoantibodies are of the IgM class. 
C)  Antibodies have a titer greater than 64 at 4°C.. 
D)  Autoantibodies can activate complement in vitro 
A)  Antibodies react best with ficin-treated cells. 
B)  Autoantibodies are of the IgM class. 
C)  Antibodies have a titer greater than 64 at 4°C.. 
D)  Autoantibodies can activate complement in vitro

Question 19

RBCs sensitized in which drug-induced hemolytic anemia mechanism act as "innocent bystanders"?

Accepted Answer

A)  Membrane modification 
B)  Immune complex 
C)  Drug absorption 
D)  Autoantibody formation 
A)  Membrane modification 
B)  Immune complex 
C)  Drug absorption 
D)  Autoantibody formation

Question 20

All of the following are clinical manifestations of cold hemagglutinin disease (CHD) except:

Accepted Answer

A)  agglutination of red blood cells in skin capillaries. 
B)  acrocyanosis of the hands and feet. 
C)  hepatosplenomegaly. 
D)  Raynaud's phenomena. 
A)  agglutination of red blood cells in skin capillaries. 
B)  acrocyanosis of the hands and feet. 
C)  hepatosplenomegaly. 
D)  Raynaud's phenomena.

Exam 21: Autoimmune Hemolytic Anemias

In warm autoimmune hemolytic anemia, the autoantibody will frequently demonstrate -like specificity.

What is the most frequent antibody specificity in CHD?

Anti-K was identified in absorbed serum of a patient with warm autoimmune hemolytic anemia. If a red blood cell phenotype was performed using an indirect antiglobulin test to ensure the patient was negative for K antigen, what would the technologist find?

A 28-year-old female with cold hemagglutinin disease has a positive DAT. When the DAT is repeated using monospecific reagent, which of the following is most likely to be detected?

How can persons with CHD avoid hemolytic episodes?

In the case of an AB-positive individual, what test must be performed to ensure that a warm-reacting autoantibody is not causing false-positive reactions?

Which of the following is a characteristic of autoantibodies?

What is the primary goal for treatment in patients with warm autoimmune hemolytic anemia?

Which of the following can be found in warm autoimmune hemolytic anemia in the presence of hypoplastic marrow?

Which alloantibody is frequently present in the serum of i adults?

Which of the following regarding the immune complex mechanism in serologic testing is true?

Which of the following describes a cold autologous absorption procedure?

Approximately what percentage of warm autoimmune hemolytic anemia will produce a positive DAT with both IgG and C3d antibodies?

Which of the following is a characteristic red blood cell morphology seen on a peripheral blood smear from a patient with warm autoimmune hemolytic anemia?

The onset of warm autoimmune hemolytic anemia (WAIHA) may be precipitated by:

In the digitonin-acid elution procedure, what is the purpose of adding phosphate buffer to the eluate solution?

How might a technologist detect a patient with drug-induced hemolytic anemia?

All of the following are characteristics of benign cold autoagglutinins except:

RBCs sensitized in which drug-induced hemolytic anemia mechanism act as "innocent bystanders"?

All of the following are clinical manifestations of cold hemagglutinin disease (CHD) except:

Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends

Basic Genetics

Fundamentals of Immunology

Concepts in Molecular Biology

The Antiglobulin Test

The Abo Blood Group System

The Rh Blood Group System

Blood Group Terminology and Common Blood Groups

Uncommon Blood Groups

Detection and Identification of Antibodies

Pre-Transfusion Testing

Blood Bank Technologies and Automation

Donor Selection

Transfusion-Transmitted Diseases

Component Preparation

Transfusion Therapy

Adverse Effects of Blood Transfusion

Apheresis

Cellular Therapy in the Transplant Setting

Hemolytic Disease of the Fetus and Newborn

Tissue Banking As Part of the Transfusion Service

The Hla System

Relationship Testing

Quality Management

Patient Blood Management

Transfusion Safety and Federal Regulatory Requirements

Laboratory Information Systems in the Blood Bank

Medicolegal and Ethical Aspects of Providing Blood Collection and Transfusion Services

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