Question 1

Match each description with the most appropriate term.
-method that copies fragments of DNA using living cells

Accepted Answer

A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic 
A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic

Question 2

Match each scenario with the type of genetic engineering that best describes it.
-Zebrafish are engineered to glow in places where BPA is present, to study the effects of the endocrine-disrupting chemical.

Accepted Answer

A) medical application 
B) research application 
C) agricultural application 
A) medical application 
B) research application 
C) agricultural application

Question 3

About ____ percent of an individual's genes are the same as in the general population.

Accepted Answer

A) 19 
B) 29 
C) 59 
D) 79 
E) 99 
A) 19 
B) 29 
C) 59 
D) 79 
E) 99

Question 4

Because it does not contain introns, researchers prefer to use ____ when working with human genes.

Accepted Answer

A) cDNA 
B) cloned DNA 
C) hybridized DNA 
D) recombinant DNA 
E) viral DNA 
A) cDNA 
B) cloned DNA 
C) hybridized DNA 
D) recombinant DNA 
E) viral DNA

Question 5

Match each description with the most appropriate term.
-any DNA copied from mRNA transcripts

Accepted Answer

A) cDNA 
B) a restriction enzyme 
C) reverse transcriptase 
D) a DNA library 
A) cDNA 
B) a restriction enzyme 
C) reverse transcriptase 
D) a DNA library

Question 6

The Ti plasmid from which plant pathogen is used to transfer genes into plants?

Accepted Answer

A) Agrobacterium tumefaciens 
B) Escherichia coli 
C) Haemophilus influenzae 
D) Salmonella typhimurium 
E) Streptococcus faecalis 
A) Agrobacterium tumefaciens 
B) Escherichia coli 
C) Haemophilus influenzae 
D) Salmonella typhimurium 
E) Streptococcus faecalis

Question 7

On an SNP chip, a yellow dot indicates that the individual ____.

Accepted Answer

A) ​is homozygous dominant for two copies of the SNP 
B) ​contains three different copies of the SNP 
C) ​is homozygous recessive for two copies of the SNP 
D) ​is heterozygous for the SNP 
E) ​does not have the SNP​ 
A) ​is homozygous dominant for two copies of the SNP 
B) ​contains three different copies of the SNP 
C) ​is homozygous recessive for two copies of the SNP 
D) ​is heterozygous for the SNP 
E) ​does not have the SNP​

Question 8

Explain how CRISPR works and how it can be used in genome editing.

Accepted Answer

CRISPR is based on a type of restriction

Question 9

Match each description with the most appropriate term.
-technique that separates DNA fragments by size

Accepted Answer

A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic 
A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic

Question 10

Describe how DNA sequencing is performed.

Accepted Answer

DNA sequencing depends on dideoxynucleot

Question 11

Explain the difference between a GMO and a transgenic organism.

Accepted Answer

An individual whose genome has been deli

Question 12

Why does DNA fingerprinting work for identifying humans?

Accepted Answer

In the noncoding regions of DNA, humans

Question 13

-In the accompanying figure, in which step are cells being ruptured to release their DNA?

Accepted Answer

A) A 
B) B 
C) C 
D) D 
E) E 
A) A 
B) B 
C) C 
D) D 
E) E

Question 14

Imagine you have paid for a genetic test from a company that can report your probability of developing Alzheimer's disease based on the presence of a certain allele. This test would be examining your ____ profile.

Accepted Answer

A) chromosome number 
B) introns 
C) RNA 
D) SNPs 
E) mRNA expression 
A) chromosome number 
B) introns 
C) RNA 
D) SNPs 
E) mRNA expression

Question 15

Match each description with the most appropriate term.
-analysis of the unique parts of a person's DNA

Accepted Answer

A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic 
A) polymerase chain reaction (PCR) 
B) DNA cloning 
C) DNA sequencing 
D) DNA profiling 
E) electrophoresis
F)probe
G)transgenic

Question 16

​In gel electrophoresis, the ____ the DNA fragment, the faster it moves through the gel.

Accepted Answer

A) more charged​ 
B) ​longer 
C) ​shorter 
D) ​higher G-C content 
E) ​higher A-T content 
A) more charged​ 
B) ​longer 
C) ​shorter 
D) ​higher G-C content 
E) ​higher A-T content

Question 17

What treatment involves the transfer of a normal gene into cells of an individual with the intent to correct a genetic defect or treat a disease?

Accepted Answer

A) DNA cloning 
B) DNA sequencing 
C) gene replacement 
D) gene therapy 
E) reverse transcriptase 
A) DNA cloning 
B) DNA sequencing 
C) gene replacement 
D) gene therapy 
E) reverse transcriptase

Question 18

Match each description with the most appropriate term.
-cuts apart foreign DNA as its primary function

Accepted Answer

A) cDNA 
B) a restriction enzyme 
C) reverse transcriptase 
D) a DNA library 
A) cDNA 
B) a restriction enzyme 
C) reverse transcriptase 
D) a DNA library

Question 19

Gene therapy is a promising therapeutic tool; however, some unwanted side effects can occur. What did some patients being treated for SCID-X1 develop after gene therapy?

Accepted Answer

A) a defective immune system 
B) leukemia 
C) myeloma 
D) viral infection 
E) diabetes 
A) a defective immune system 
B) leukemia 
C) myeloma 
D) viral infection 
E) diabetes

Question 20

​DNA sequencing gives a readout of 5' AATCGGTTG 3' based on the color-labeled nucleotides detected after gel electrophoresis. What is the sequence of the template strand?

Accepted Answer

A) ​5' AATCGGTTG 3' 
B) ​5' CAACCGATT 3' 
C) ​5' TTAGCCAAC 3' 
D) ​5' GTTGGCTAA 3' 
E) ​More information is required to determine the sequence of the template strand. 
A) ​5' AATCGGTTG 3' 
B) ​5' CAACCGATT 3' 
C) ​5' TTAGCCAAC 3' 
D) ​5' GTTGGCTAA 3' 
E) ​More information is required to determine the sequence of the template strand.

Exam 15: Biotechnology

Match each description with the most appropriate term. -method that copies fragments of DNA using living cells

Match each scenario with the type of genetic engineering that best describes it. -Zebrafish are engineered to glow in places where BPA is present, to study the effects of the endocrine-disrupting chemical.

About ____ percent of an individual's genes are the same as in the general population.

Because it does not contain introns, researchers prefer to use ____ when working with human genes.

Match each description with the most appropriate term. -any DNA copied from mRNA transcripts

The Ti plasmid from which plant pathogen is used to transfer genes into plants?

On an SNP chip, a yellow dot indicates that the individual ____.

Explain how CRISPR works and how it can be used in genome editing.

Match each description with the most appropriate term. -technique that separates DNA fragments by size

Describe how DNA sequencing is performed.

Explain the difference between a GMO and a transgenic organism.

Why does DNA fingerprinting work for identifying humans?

-In the accompanying figure, in which step are cells being ruptured to release their DNA?

Imagine you have paid for a genetic test from a company that can report your probability of developing Alzheimer's disease based on the presence of a certain allele. This test would be examining your ____ profile.

Match each description with the most appropriate term. -analysis of the unique parts of a person's DNA

​In gel electrophoresis, the ____ the DNA fragment, the faster it moves through the gel.

What treatment involves the transfer of a normal gene into cells of an individual with the intent to correct a genetic defect or treat a disease?

Match each description with the most appropriate term. -cuts apart foreign DNA as its primary function

Gene therapy is a promising therapeutic tool; however, some unwanted side effects can occur. What did some patients being treated for SCID-X1 develop after gene therapy?

​DNA sequencing gives a readout of 5' AATCGGTTG 3' based on the color-labeled nucleotides detected after gel electrophoresis. What is the sequence of the template strand?

The Science of Biology

Lifes Chemical Basis

Molecules of Life

Cell Structure

Ground Rules of Metabolism

Where It Starts-Photosynthesis

Releasing Chemical Energy

DNA Structure and Function

From DNA to Protein

Control of Gene Expression

How Cells Reproduce

Meiosis and Sexual Reproduction

Patterns in Inherited Traits

Human Inheritance

Evidence of Evolution

Processes of Evolution

Lifes Origin and Early Evolution

Viruses, Bacteria, and Archaea

The Protists

Plant Evolution

Fungi

Animals I: Major Invertebrate Groups

Animals Ii: the Chordates

Plant Tissues

Plant Nutrition and Transport

Plant Reproduction and Development

Animal Tissues and Organ Systems

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Reproduction and Development

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Filters

In gel electrophoresis, the ____ the DNA fragment, the faster it moves through the gel.

DNA sequencing gives a readout of 5' AATCGGTTG 3' based on the color-labeled nucleotides detected after gel electrophoresis. What is the sequence of the template strand?