Exam 20: Recombinant Dna Technology

If one wishes to clone a gene using typical restriction endonucleases,how does the restriction endonuclease identify the appropriate cut sites in the genome?

The development of polymerase chain reaction PCR)was a watershed event in recombinant DNA technology.What are two characteristics of PCR that make it such an important development?

What is the order of the three main steps in a PCR?

There are multiple cloning vector types in modern recombinant DNA technology ranging from plasmids to viral vectors.Which vector type is most useful when cloning an insert of approximately 500kb? A)bacterial plasmid B)human artificial chromosome C)yeast artificial chromosome D)viral vector E)bacterial artificial chromosome

Assume that a plasmid circular is 2800 base pairs in length and has restriction sites for EcoRI at the following locations: 400,700,1400,and 2600.Give the expected sizes of the restriction fragments following complete digestion with EcoRI.

When generating a cDNA library,one may get different sequences returned if samples are taken at different times.Why is this the case?

The CRISPR- Cas system of gene editing is based on what naturally occurring biological process?

The genomics era began with the development of which of the following?

A plasmid contains a multiple cloning site inside the coding region of the lacZ gene and also contains an ampicillin resistance gene at a separate locus.When cells are transformed with a successfully recombinant plasmid containing a piece of DNA cloned into the multiple cloning site,what color will the colonies be when grown on an agar plate containing ampicillin and X- gal?

What is the most serious drawback to using library screening to study genetics?

Cloned DNA is useful in studying which areas)of genetic manipulation?

In a PCR,the polymerase adds nucleotides to the 3'- hydroxyl of the terminal nucleotide of each primer.Briefly describe how this results in the amplification of the single region of interest in the genome.