Exam 21: Genomic Analysis

What does MALDI stand for?

A common first separation technique in 2D gels is isoelectric focusing.What property is isoelectric focusing taking advantage of?

A newly sequenced gene is cloned and expressed in a bacterial cell.The bioinformatics databases suggest that this gene produces an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate.After weeks of experiments,a scientist determined the enzyme has a completely different function.This is an example of_______ .

Numerous scientists around the world have proposed to sequence 10,000 vertebrate genomes in five years.What is the name of this plan?

A drawback to using microarrays is_______ .

Personal genome sequencing assists with the recognition and determination of what?

DNA microarray analysis is excellent for _______.

Several years ago,scientists compared the genome of a wooly mammoth to that of an African elephant and found them to be 98.5% identical.This is an example of what type of "- omics"?

Genomes from several primates have been sequenced and annotated.These primates range from chimpanzee to Rhesus monkey to Neanderthal and modern humans.What is the advantage of having so many primate sequences available to researchers?

A microbiome is essential for human infant development.Where does an infant obtain the origins of its microbiome?

Most of the bacterial genomes described in the text have fewer than_______ .

Nutrigenomics is the study of_______ .

In general,the organization of genes in bacteria is different from that in eukaryotes.In E.coli,approximately 27 percent of all genes are organized into contiguous,functionally related units containing multiple genes under coordinated control that are transcribed as a single unit.Such contiguous gene families are called_______ .

Compared with eukaryotic chromosomes,bacterial chromosomes are_______ .

What is one cause for the 21,000 protein encoding sequences in the human genome producing between 200,000 and 1 million different proteins?

Transcriptomics is _______.

The genome of the domesticated dog,Canis familiaris,was sequenced in 2005.Using the dog as a model system provides insight into which of the following human disease states?

Using RNA- seq generates data in situ.What does RNA- seq not provide data for?

One major difference between prokaryotic and eukaryotic genes is that eukaryotic genes can contain internal sequences,called_______ ,which get removed in the mature message.

Transcriptomics requires the isolation of RNA to generate sequence data.If a scientist wants to use next- generation sequencing to perform their experiment which of the following is necessary?