Question 1

What active site cofactor is found in the electron transport system protein called cytochrome c?

Accepted Answer

A)  heme 
B)  FeS cluster 
C)  Flavin 
D)  quinone 
A)  heme 
B)  FeS cluster 
C)  Flavin 
D)  quinone

Question 2

The drug oligomycin inhibits ATP synthase by preventing protons from flowing through the enzyme.Oligomycin must bind to the __________ of ATP synthase.

Accepted Answer

A)  catalytic headpiece 
B)  F1 subunit 
C)  ATP binding site 
D)  F0 subunit of 
A)  catalytic headpiece 
B)  F1 subunit 
C)  ATP binding site 
D)  F0 subunit of

Question 3

What is the cellular location of eukaryotic thermogenin (or uncoupling protein)?

Accepted Answer

A)  mitochondrial inner membrane 
B)  mitochondrial matrix 
C)  cytoplasm 
D)  both cytoplasm and mitochondrial matrix 
A)  mitochondrial inner membrane 
B)  mitochondrial matrix 
C)  cytoplasm 
D)  both cytoplasm and mitochondrial matrix

Question 4

What would happen to a mutated ATP synthase enzyme where the proton binding aspartate residue on the c subunits was mutated to an alanine?

Accepted Answer

A)  The enzyme would make ATP as normal in the presence of a proton gradient. 
B)  The enzyme would not make ATP in the presence of a proton gradient. 
C)  The enzyme would make ATP without the need for a proton gradient. 
D)  The rotor would rotate in response to a proton gradient, but no ATP would be made. 
A)  The enzyme would make ATP as normal in the presence of a proton gradient. 
B)  The enzyme would not make ATP in the presence of a proton gradient. 
C)  The enzyme would make ATP without the need for a proton gradient. 
D)  The rotor would rotate in response to a proton gradient, but no ATP would be made.

Question 5

Which of the following is NOT a part of oxidative phosphorylation?

Accepted Answer

A)  NADH is oxidized to NAD+. 
B)  O2 is reduced to H2O. 
C)  Electrons from O2 are transferred to ATP. 
D)  The pumping of protons is coupled with the formation of ATP. 
A)  NADH is oxidized to NAD+. 
B)  O2 is reduced to H2O. 
C)  Electrons from O2 are transferred to ATP. 
D)  The pumping of protons is coupled with the formation of ATP.

Question 6

Protons in the mitochondria are

Accepted Answer

A)  the driving force for the electron transfers in the electron transport system. 
B)  pumped by mitochondrial electron transport system enzymes. 
C)  pumped inside the mitochondria using ATP energy. 
D)  the cause of a lower pH inside the mitochondria than outside the mitochondria. 
A)  the driving force for the electron transfers in the electron transport system. 
B)  pumped by mitochondrial electron transport system enzymes. 
C)  pumped inside the mitochondria using ATP energy. 
D)  the cause of a lower pH inside the mitochondria than outside the mitochondria.

Question 7

What are the principle physiological electron donors for the mitochondrial electron transport pathway?

Accepted Answer

A)  FADH2 and NADH 
B)  FADH2, NADH, and NADPH 
C)  NADH only 
D)  UQH2, NADH, and FADH2 
A)  FADH2 and NADH 
B)  FADH2, NADH, and NADPH 
C)  NADH only 
D)  UQH2, NADH, and FADH2

Question 8

List the order of enzymes,enzyme complexes,and membrane soluble electron carriers that an electron is passed through on its way from NADH to O2 in the electron transport system.

Accepted Answer

NADH @#IMG-DLM&_TB6697_11complex I @#IMG-DLM&_TB66

Question 9

Brown adipose tissue is brown in color because of

Accepted Answer

A)  increased levels of thermogenin, or uncoupling protein. 
B)  increased muscle fiber content. 
C)  increased numbers of mitochondria. 
D)  decreased levels of lipid. 
A)  increased levels of thermogenin, or uncoupling protein. 
B)  increased muscle fiber content. 
C)  increased numbers of mitochondria. 
D)  decreased levels of lipid.

Question 10

What type of transport is illustrated by the mitochondrial ATP/ADP translocase?

Accepted Answer

A)  symporter 
B)  antiporter 
C)  facilitated diffusion 
D)  primary active transporter 
A)  symporter 
B)  antiporter 
C)  facilitated diffusion 
D)  primary active transporter

Question 11

An ATP synthase enzyme with more than 10 c subunits in the F0 stalk would

Accepted Answer

A)  require more protons to complete one 360    Rotation. 
B)  result in more ATP synthesis per 360    Turn. 
C)  require fewer protons to rotate 360     
D)  result in less ATP synthesis per 360    Turn. 
A)  require more protons to complete one 360    Rotation. 
B)  result in more ATP synthesis per 360    Turn. 
C)  require fewer protons to rotate 360     
D)  result in less ATP synthesis per 360    Turn.

Question 12

The main activator molecule of the ATP synthesis and the electron transport system is

Accepted Answer

A)  ATP. 
B)  H+. 
C)  ADP. 
D)  NAD+. 
A)  ATP. 
B)  H+. 
C)  ADP. 
D)  NAD+.

Question 13

Explain how ATP synthase can be used as primary active transporter of H+ and pump protons.

Accepted Answer

ATP synthase can be run in the reverse d

Question 14

What is the location of the electron transport system protein called cytochrome c?

Accepted Answer

A)  cytoplasm 
B)  mitochondrial matrix 
C)  inner mitochondrial membrane 
D)  intermembrane space 
A)  cytoplasm 
B)  mitochondrial matrix 
C)  inner mitochondrial membrane 
D)  intermembrane space

Question 15

Thermogenin (or uncoupling protein)is not toxic like 2,4-dinitrophenol because the protein

Accepted Answer

A)  is typically expressed in adipose tissues where ample fat calories are found. 
B)  is less efficient at proton dissipation. 
C)  cannot leave the mitochondria. 
D)  is naturally occurring and not synthetic. 
A)  is typically expressed in adipose tissues where ample fat calories are found. 
B)  is less efficient at proton dissipation. 
C)  cannot leave the mitochondria. 
D)  is naturally occurring and not synthetic.

Question 16

Explain why many mitochondrial diseases are inherited from the mother only.

Accepted Answer

Many of the proteins in the mitochondria

Question 17

What would happen if mitochondria were treated with a proton gradient uncoupler,such as 2,4-dinitrophenol?

Accepted Answer

A)  Electron transfer would stop. 
B)  Complex I would become reduced, and complexes III and IV would become oxidized. 
C)  Protons would be pumped by the mitochondrial electron transport chain, although no ATP would be synthesized. 
D)  Reducing equivalents, in the form of NADH, would no longer be consumed. 
A)  Electron transfer would stop. 
B)  Complex I would become reduced, and complexes III and IV would become oxidized. 
C)  Protons would be pumped by the mitochondrial electron transport chain, although no ATP would be synthesized. 
D)  Reducing equivalents, in the form of NADH, would no longer be consumed.

Question 18

Which of the following statements about the chemiosmotic theory is true?

Accepted Answer

A)  It requires an enclosed mitochondrial membrane. 
B)  The membrane ATPase (or ATP synthase) has no significant role in the theory. 
C)  Energy is coupled through a transmembrane electron gradient. 
D)  It explains how ATP energy is used to create a pH gradient. 
A)  It requires an enclosed mitochondrial membrane. 
B)  The membrane ATPase (or ATP synthase) has no significant role in the theory. 
C)  Energy is coupled through a transmembrane electron gradient. 
D)  It explains how ATP energy is used to create a pH gradient.

Question 19

Which mechanism is the most efficient at moving NADH equivalents from the cytoplasm into the mitochondria?

Accepted Answer

A)  citrate shuttle 
B)  malate-aspartate shuttle 
C)  glyoxylate shunt 
D)  glycerol phosphate shuttle 
A)  citrate shuttle 
B)  malate-aspartate shuttle 
C)  glyoxylate shunt 
D)  glycerol phosphate shuttle

Question 20

List the common enzyme names of the major enzyme complexes in the oxidative phosphorylation pathway,which are required to couple NADH oxidation with ATP synthesis.

Accepted Answer

Complex I (NADH-ubiquinone oxi

Exam 11: Oxidative Phosphorylation

What active site cofactor is found in the electron transport system protein called cytochrome c?

The drug oligomycin inhibits ATP synthase by preventing protons from flowing through the enzyme.Oligomycin must bind to the __________ of ATP synthase.

What is the cellular location of eukaryotic thermogenin (or uncoupling protein)?

What would happen to a mutated ATP synthase enzyme where the proton binding aspartate residue on the c subunits was mutated to an alanine?

Which of the following is NOT a part of oxidative phosphorylation?

Protons in the mitochondria are

What are the principle physiological electron donors for the mitochondrial electron transport pathway?

List the order of enzymes,enzyme complexes,and membrane soluble electron carriers that an electron is passed through on its way from NADH to O2 in the electron transport system.

Brown adipose tissue is brown in color because of

What type of transport is illustrated by the mitochondrial ATP/ADP translocase?

An ATP synthase enzyme with more than 10 c subunits in the F0 stalk would

The main activator molecule of the ATP synthesis and the electron transport system is

Explain how ATP synthase can be used as primary active transporter of H+ and pump protons.

What is the location of the electron transport system protein called cytochrome c?

Thermogenin (or uncoupling protein)is not toxic like 2,4-dinitrophenol because the protein

Explain why many mitochondrial diseases are inherited from the mother only.

What would happen if mitochondria were treated with a proton gradient uncoupler,such as 2,4-dinitrophenol?

Which of the following statements about the chemiosmotic theory is true?

Which mechanism is the most efficient at moving NADH equivalents from the cytoplasm into the mitochondria?

List the common enzyme names of the major enzyme complexes in the oxidative phosphorylation pathway,which are required to couple NADH oxidation with ATP synthesis.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

List the order of enzymes,enzyme complexes,and membrane soluble electron carriers that an electron is passed through on its way from NADH to O₂ in the electron transport system.

An ATP synthase enzyme with more than 10 c subunits in the F₀ stalk would

Explain how ATP synthase can be used as primary active transporter of H⁺ and pump protons.