Question 1

The preparation of knockout mutants involves:

Accepted Answer

A)  inserting a piece of DNA into the gene of interest. 
B)  the use of closely packed DNA fragments attached to a glass slide. 
C)  searching for homology. 
D)  determining the number of base pairs in a gene. 
E)  determining the rate of recombination. 
A)  inserting a piece of DNA into the gene of interest. 
B)  the use of closely packed DNA fragments attached to a glass slide. 
C)  searching for homology. 
D)  determining the number of base pairs in a gene. 
E)  determining the rate of recombination.

Question 2

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

Accepted Answer

A)  particle bombardment 
B)  the polymerase chain reaction 
C)  a reaction involving reverse transcriptase 
D)  electrophoresis 
E)  electroporation 
A)  particle bombardment 
B)  the polymerase chain reaction 
C)  a reaction involving reverse transcriptase 
D)  electrophoresis 
E)  electroporation

Question 3

One advantage of inserting into crop plants a gene that codes for an altered enzyme in aromatic amino acid synthesis is that the plants would:

Accepted Answer

A)  be resistant to BT toxin. 
B)  be resistant to glyphosphate. 
C)  be resistant to Bacillus thuringiensis. 
D)  have an increased starch content. 
E)  have an increased vitamin A content. 
A)  be resistant to BT toxin. 
B)  be resistant to glyphosphate. 
C)  be resistant to Bacillus thuringiensis. 
D)  have an increased starch content. 
E)  have an increased vitamin A content.

Question 4

An example of a selectable marker gene is:

Accepted Answer

A)  the gene for a green fluorescent protein. 
B)  the luciferase gene. 
C)  EcoRI. 
D)  lacZ. 
E)  ampR. 
A)  the gene for a green fluorescent protein. 
B)  the luciferase gene. 
C)  EcoRI. 
D)  lacZ. 
E)  ampR.

Question 5

Most of the genes in all bacteria are significantly similar.

Accepted Answer

A) True 
 B)False

Question 6

A knockout mutant has a particular gene inactivated.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following is NOT one of the goals of functional genomics?

Accepted Answer

A)  identifying the transcriptome 
B)  identifying the proteome 
C)  determining the function of genes 
D)  determining which genes are expressed 
E)  determining the location of genes on a chromosome 
A)  identifying the transcriptome 
B)  identifying the proteome 
C)  determining the function of genes 
D)  determining which genes are expressed 
E)  determining the location of genes on a chromosome

Question 8

Which of the following statements concerning restriction enzymes is FALSE?

Accepted Answer

A)  They are a type of endonuclease. 
B)  They recognize DNA sequences typically four to six nucleotides long. 
C)  They recognize single-stranded DNA sequences. 
D)  Some make straight cuts in the DNA. 
E)  They recognize specific palindromic sequences. 
A)  They are a type of endonuclease. 
B)  They recognize DNA sequences typically four to six nucleotides long. 
C)  They recognize single-stranded DNA sequences. 
D)  Some make straight cuts in the DNA. 
E)  They recognize specific palindromic sequences.

Question 9

One aspect of structural genomics is:

Accepted Answer

A)  determining the function of genes. 
B)  determining the function of gene products. 
C)  preparing physical maps. 
D)  preparing knockout mutants. 
E)  comparing evolutionary relationships. 
A)  determining the function of genes. 
B)  determining the function of gene products. 
C)  preparing physical maps. 
D)  preparing knockout mutants. 
E)  comparing evolutionary relationships.

Question 10

A sticky-ended fragment can join with another DNA segment only if that segment:

Accepted Answer

A)  has been cut with the same restriction enzyme. 
B)  has been cut with the same ligase. 
C)  is palindromic. 
D)  is part of a plasmid. 
E)  is part of a virus. 
A)  has been cut with the same restriction enzyme. 
B)  has been cut with the same ligase. 
C)  is palindromic. 
D)  is part of a plasmid. 
E)  is part of a virus.

Question 11

Which of the following statements concerning vectors is FALSE?

Accepted Answer

A)  Vectors are used to transform cells. 
B)  Vectors function as DNA carriers. 
C)  A virus can be a vector. 
D)  A plasmid can be a vector. 
E)  A host cell can be a vector. 
A)  Vectors are used to transform cells. 
B)  Vectors function as DNA carriers. 
C)  A virus can be a vector. 
D)  A plasmid can be a vector. 
E)  A host cell can be a vector.

Question 12

The Taq polymerase is used in recombinant DNA technology to:

Accepted Answer

A)  locate specific DNA segments. 
B)  determine the nucleotide sequence of a specific gene. 
C)  make up to a millionfold copies of a DNA segment in a few hours. 
D)  make cDNA. 
E)  carry out "shotgun cloning." 
A)  locate specific DNA segments. 
B)  determine the nucleotide sequence of a specific gene. 
C)  make up to a millionfold copies of a DNA segment in a few hours. 
D)  make cDNA. 
E)  carry out "shotgun cloning."

Question 13

The proteome refers to all the protein molecules transcribed by the DNA.

Accepted Answer

A) True 
 B)False

Question 14

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Accepted Answer

A) True 
 B)False

Question 15

Because meristems lack _________ tissues, meristem culture results in plants that lack _________.

Accepted Answer

A)  vascular; viruses 
B)  vascular; fungi 
C)  epidermal; viruses 
D)  epidermal; fungi 
E)  epidermal; insect eggs 
A)  vascular; viruses 
B)  vascular; fungi 
C)  epidermal; viruses 
D)  epidermal; fungi 
E)  epidermal; insect eggs

Question 16

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

Accepted Answer

A)  auxin synthesis only. 
B)  opine synthesis only. 
C)  cytokinin synthesis only. 
D)  auxin and cytokinin synthesis. 
E)  auxin and opine synthesis. 
A)  auxin synthesis only. 
B)  opine synthesis only. 
C)  cytokinin synthesis only. 
D)  auxin and cytokinin synthesis. 
E)  auxin and opine synthesis.

Question 17

DNA ligase would most likely be used in recombinant DNA technology to:

Accepted Answer

A)  function as a vector. 
B)  screen for recombinant plasmids. 
C)  stimulate a transformed cell to divide. 
D)  cut DNA into fragments with sticky ends. 
E)  join DNA fragments together. 
A)  function as a vector. 
B)  screen for recombinant plasmids. 
C)  stimulate a transformed cell to divide. 
D)  cut DNA into fragments with sticky ends. 
E)  join DNA fragments together.

Question 18

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

Accepted Answer

A) True 
 B)False

Question 19

The BT gene is transferred for the purpose of:

Accepted Answer

A)  delaying leaf senescence. 
B)  reducing the proportion of saturated fats. 
C)  conferring resistance to caterpillars. 
D)  conferring resistance to herbicides. 
E)  delaying fruit ripening. 
A)  delaying leaf senescence. 
B)  reducing the proportion of saturated fats. 
C)  conferring resistance to caterpillars. 
D)  conferring resistance to herbicides. 
E)  delaying fruit ripening.

Question 20

Micropropagation is used to obtain clones of plants.

Accepted Answer

A) True 
 B)False

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

The preparation of knockout mutants involves:

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

One advantage of inserting into crop plants a gene that codes for an altered enzyme in aromatic amino acid synthesis is that the plants would:

An example of a selectable marker gene is:

Most of the genes in all bacteria are significantly similar.

A knockout mutant has a particular gene inactivated.

Which of the following is NOT one of the goals of functional genomics?

Which of the following statements concerning restriction enzymes is FALSE?

One aspect of structural genomics is:

A sticky-ended fragment can join with another DNA segment only if that segment:

Which of the following statements concerning vectors is FALSE?

The Taq polymerase is used in recombinant DNA technology to:

The proteome refers to all the protein molecules transcribed by the DNA.

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Because meristems lack _________ tissues, meristem culture results in plants that lack _________.

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

DNA ligase would most likely be used in recombinant DNA technology to:

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

The BT gene is transferred for the purpose of:

Micropropagation is used to obtain clones of plants.

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

Because meristems lack _ tissues, meristem culture results in plants that lack _.