Question 1

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Accepted Answer

A) True 
 B)False

Question 2

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 3

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Accepted Answer

A) True 
 B)False

Question 4

One important characteristic the Taq polymerase is its

Accepted Answer

A)  insensitivity to pH. 
B)  ability to glow when in the presence of a suitable substrate. 
C)  ability to be cloned. 
D)  resistance to high temperature. 
E)  ability to transform cells. 
A)  insensitivity to pH. 
B)  ability to glow when in the presence of a suitable substrate. 
C)  ability to be cloned. 
D)  resistance to high temperature. 
E)  ability to transform cells.

Question 5

A genetic map is different from a physical map in that a genetic map is:

Accepted Answer

A)  used to determine the function of genes. 
B)  based on rates of recombination. 
C)  used to determine which genes are inactivate. 
D)  based on direct DNA sequencing. 
E)  based on evolutionary relationships. 
A)  used to determine the function of genes. 
B)  based on rates of recombination. 
C)  used to determine which genes are inactivate. 
D)  based on direct DNA sequencing. 
E)  based on evolutionary relationships.

Question 6

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Accepted Answer

A) True 
 B)False

Question 7

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

Accepted Answer

A)  R 
B)  O 
C)  onc 
D)  vir 
E)  A 
A)  R 
B)  O 
C)  onc 
D)  vir 
E)  A

Question 8

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

Accepted Answer

A)  to delay leaf senescence 
B)  to confer resistance to herbicides 
C)  to confer resistance to BT toxin 
D)  to increase starch content 
E)  to increase glyphosate production 
A)  to delay leaf senescence 
B)  to confer resistance to herbicides 
C)  to confer resistance to BT toxin 
D)  to increase starch content 
E)  to increase glyphosate production

Question 9

Traditional breeding programs:

Accepted Answer

A)  involve inserting a plasmid into a host cell. 
B)  involve infecting a host plant with a virus. 
C)  allow gene transfers between species incapable of hybridizing. 
D)  depend on inherent genetic variability. 
E)  allow the precise insertion of genes into a plant. 
A)  involve inserting a plasmid into a host cell. 
B)  involve infecting a host plant with a virus. 
C)  allow gene transfers between species incapable of hybridizing. 
D)  depend on inherent genetic variability. 
E)  allow the precise insertion of genes into a plant.

Question 10

It was not until the discovery of ________ that organ and tissue culture became feasible.

Accepted Answer

A)  hydroponics 
B)  plant hormones 
C)  the importance of sucrose in nutrient solutions 
D)  the essential mineral elements 
E)  biotechnology 
A)  hydroponics 
B)  plant hormones 
C)  the importance of sucrose in nutrient solutions 
D)  the essential mineral elements 
E)  biotechnology

Question 11

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Accepted Answer

A) True 
 B)False

Question 12

By definition, hydroponics is the technique for growing:

Accepted Answer

A)  shoot tips. 
B)  isolated leaf pieces. 
C)  isolated roots 
D)  plant embryos. 
E)  plants without soil. 
A)  shoot tips. 
B)  isolated leaf pieces. 
C)  isolated roots 
D)  plant embryos. 
E)  plants without soil.

Question 13

Which of the following statements about eukaryotic genomes is FALSE?

Accepted Answer

A)  They are larger than those of prokaryotes. 
B)  In general, multicellular eukaryotes have more DNA than unicellular eukaryotes. 
C)  The greater the complexity of the organism, the more protein-coding genes it has. 
D)  The roundworm Caenorhacditis has more genes than the fruit fly Drosophila. 
E)  The plant Arabidopsis has more genes than the fruit fly Drosophila. 
A)  They are larger than those of prokaryotes. 
B)  In general, multicellular eukaryotes have more DNA than unicellular eukaryotes. 
C)  The greater the complexity of the organism, the more protein-coding genes it has. 
D)  The roundworm Caenorhacditis has more genes than the fruit fly Drosophila. 
E)  The plant Arabidopsis has more genes than the fruit fly Drosophila.

Question 14

Agrobacterium tumefaciens is used to obtain transgenetic plants.

Accepted Answer

A) True 
 B)False

Question 15

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Accepted Answer

A) True 
 B)False

Question 16

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

Accepted Answer

A) True 
 B)False

Question 17

One advantage of meristem culture is that virus-free plants can be obtained.

Accepted Answer

A) True 
 B)False

Question 18

Sticky-ended fragments can be joined by specific restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following statements concerning DNA libraries is FALSE?

Accepted Answer

A)  A genomic library is obtained by cutting the entire genome of an organism. 
B)  A cDNA library is obtained by using reverse transcriptase. 
C)  A DNA library contains cloned fragments of most of an organism's genome. 
D)  A cDNA library lacks introns. 
E)  A genomic library lacks exons. 
A)  A genomic library is obtained by cutting the entire genome of an organism. 
B)  A cDNA library is obtained by using reverse transcriptase. 
C)  A DNA library contains cloned fragments of most of an organism's genome. 
D)  A cDNA library lacks introns. 
E)  A genomic library lacks exons.

Question 20

Cells containing the gene ampR would not survive when incubated on a medium containing ampicillin.

Accepted Answer

A) True 
 B)False

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

One important characteristic the Taq polymerase is its

A genetic map is different from a physical map in that a genetic map is:

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

Traditional breeding programs:

It was not until the discovery of ________ that organ and tissue culture became feasible.

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

By definition, hydroponics is the technique for growing:

Which of the following statements about eukaryotic genomes is FALSE?

Agrobacterium tumefaciens is used to obtain transgenetic plants.

When you grow a plant hydroponically, by definition you grow it without its roots in water.

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

One advantage of meristem culture is that virus-free plants can be obtained.

Sticky-ended fragments can be joined by specific restriction enzymes.

Which of the following statements concerning DNA libraries is FALSE?

Cells containing the gene ampR would not survive when incubated on a medium containing ampicillin.

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

Cells containing the gene amp^R would not survive when incubated on a medium containing ampicillin.