Question 1

The transcriptome refers to all of the:

Accepted Answer

A)  proteins encoded by the genome. 
B)  glycoproteins transcribed by the genome. 
C)  nucleotides present in a cell's DNA. 
D)  nucleotides present in a cell's RNA. 
E)  RNA molecules transcribed by the genome. 
A)  proteins encoded by the genome. 
B)  glycoproteins transcribed by the genome. 
C)  nucleotides present in a cell's DNA. 
D)  nucleotides present in a cell's RNA. 
E)  RNA molecules transcribed by the genome. E

Question 2

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

Accepted Answer

A) True 
 B)False  True

Question 3

In recombinant DNA technology, reverse transcriptase is used to:

Accepted Answer

A)  synthesize cDNA. 
B)  synthesize mRNA. 
C)  cut DNA into fragments. 
D)  amplify a specific gene. 
E)  transfer a plasmid into a cell. 
A)  synthesize cDNA. 
B)  synthesize mRNA. 
C)  cut DNA into fragments. 
D)  amplify a specific gene. 
E)  transfer a plasmid into a cell. A

Question 4

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

Accepted Answer

A) True 
 B)False

Question 5

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

Accepted Answer

A) True 
 B)False

Question 6

A search for homology would be used to determine the possible function of a gene.

Accepted Answer

A) True 
 B)False

Question 7

Crown-gall tumors are produced by a(n):

Accepted Answer

A)  virus. 
B)  bacterium. 
C)  fungus. 
D)  alga. 
E)  plant. 
A)  virus. 
B)  bacterium. 
C)  fungus. 
D)  alga. 
E)  plant.

Question 8

The first eukaryotic genome to be sequenced was that of:

Accepted Answer

A)  Haemophilus influenzae. 
B)  Saccharomyces cerevisae. 
C)  Arabidopsis thaliana. 
D)  Agrobacterium tumefaciens. 
E)  Caenorhabditis elegans. 
A)  Haemophilus influenzae. 
B)  Saccharomyces cerevisae. 
C)  Arabidopsis thaliana. 
D)  Agrobacterium tumefaciens. 
E)  Caenorhabditis elegans.

Question 9

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

Accepted Answer

A) True 
 B)False

Question 10

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

Accepted Answer

A)  amplify the DNA molecule. 
B)  carry out DNA hybridization. 
C)  locate a gene of interest. 
D)  determine the function of a gene. 
E)  determine the nucleotide sequence of a gene. 
A)  amplify the DNA molecule. 
B)  carry out DNA hybridization. 
C)  locate a gene of interest. 
D)  determine the function of a gene. 
E)  determine the nucleotide sequence of a gene.

Question 11

Opines are:

Accepted Answer

A)  hormones. 
B)  genes. 
C)  amino acid derivatives. 
D)  vitamins. 
E)  enzymes. 
A)  hormones. 
B)  genes. 
C)  amino acid derivatives. 
D)  vitamins. 
E)  enzymes.

Question 12

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Accepted Answer

A)  CG; CG-DNA 
B)  O; R-DNA 
C)  C; C-DNA 
D)  Ti; T-DNA 
E)  T-DNA; Ti 
A)  CG; CG-DNA 
B)  O; R-DNA 
C)  C; C-DNA 
D)  Ti; T-DNA 
E)  T-DNA; Ti

Question 13

Which of the following statements about particle bombardment is FALSE?

Accepted Answer

A)  It is used to deliver DNA but not RNA into a cell. 
B)  It involves the use of gold or tungsten beads. 
C)  It involves high-velocity microprojectiles. 
D)  It involves the use of a gene gun. 
E)  It has been used to create a virus-resistant strain of papaya. 
A)  It is used to deliver DNA but not RNA into a cell. 
B)  It involves the use of gold or tungsten beads. 
C)  It involves high-velocity microprojectiles. 
D)  It involves the use of a gene gun. 
E)  It has been used to create a virus-resistant strain of papaya.

Question 14

The ampR gene is used in recombinant DNA technology to:

Accepted Answer

A)  produce β-galactosidase. 
B)  clone a particular gene fragment. 
C)  screen for the presence of cells containing recombinant plasmids. 
D)  help the bacterium to survive on a medium containing lactose. 
E)  transform a bacterial cell. 
A)  produce β-galactosidase. 
B)  clone a particular gene fragment. 
C)  screen for the presence of cells containing recombinant plasmids. 
D)  help the bacterium to survive on a medium containing lactose. 
E)  transform a bacterial cell.

Question 15

By definition, a transformed cell:

Accepted Answer

A)  contains a plasmid. 
B)  contains a recombinant DNA molecule. 
C)  contains a virus. 
D)  functions as a vector. 
E)  divides in such a way so as to amplify the DNA fragment. 
A)  contains a plasmid. 
B)  contains a recombinant DNA molecule. 
C)  contains a virus. 
D)  functions as a vector. 
E)  divides in such a way so as to amplify the DNA fragment.

Question 16

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Accepted Answer

A)  increasing starch content. 
B)  increasing levels of β-carotene. 
C)  stimulating fruit ripening. 
D)  conferring resistance to pesticides. 
E)  delaying flower wilting. 
A)  increasing starch content. 
B)  increasing levels of β-carotene. 
C)  stimulating fruit ripening. 
D)  conferring resistance to pesticides. 
E)  delaying flower wilting.

Question 17

Suppose a plasmid containing a gene of interest plus the ampR gene is used to transform E. coli cells. When these cells are placed on a medium containing ampicillin, what will happen?

Accepted Answer

A)  They will die. 
B)  They will survive and grow. 
C)  They will form blue colonies. 
D)  They will glow with a green color. 
E)  They will glow when provided with luciferin plus ATP. 
A)  They will die. 
B)  They will survive and grow. 
C)  They will form blue colonies. 
D)  They will glow with a green color. 
E)  They will glow when provided with luciferin plus ATP.

Question 18

Which of the following statements about prokaryotic genomes is FALSE?

Accepted Answer

A)  Most consist of a single circular chromosome. 
B)  Approximately 90% of the identified genes have been assigned a function. 
C)  About 25% of the genes have no significant similarity to other known genes in bacteria. 
D)  Horizontal gene exchange periodically occurs. 
E)  Gene transfers have occurred between bacteria and eukaryotes. 
A)  Most consist of a single circular chromosome. 
B)  Approximately 90% of the identified genes have been assigned a function. 
C)  About 25% of the genes have no significant similarity to other known genes in bacteria. 
D)  Horizontal gene exchange periodically occurs. 
E)  Gene transfers have occurred between bacteria and eukaryotes.

Question 19

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

Accepted Answer

A) True 
 B)False

Question 20

When the lacZ gene technique is used to detect recombinant DNA in host bacterial cells, the colonies will appear _______ if the cells have been successfully transformed.

Accepted Answer

A)  red 
B)  white 
C)  blue 
D)  green 
E)  fluorescent 
A)  red 
B)  white 
C)  blue 
D)  green 
E)  fluorescent

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

The transcriptome refers to all of the:

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

In recombinant DNA technology, reverse transcriptase is used to:

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

A search for homology would be used to determine the possible function of a gene.

Crown-gall tumors are produced by a(n):

The first eukaryotic genome to be sequenced was that of:

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

Opines are:

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Which of the following statements about particle bombardment is FALSE?

The ampR gene is used in recombinant DNA technology to:

By definition, a transformed cell:

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Suppose a plasmid containing a gene of interest plus the ampR gene is used to transform E. coli cells. When these cells are placed on a medium containing ampicillin, what will happen?

Which of the following statements about prokaryotic genomes is FALSE?

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

When the lacZ gene technique is used to detect recombinant DNA in host bacterial cells, the colonies will appear _______ if the cells have been successfully transformed.

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

Agrobacterium induces the formation of crown-gall tumors by transferring the plasmid containing __ to the host plant's nuclear DNA.

The amp^R gene is used in recombinant DNA technology to:

Suppose a plasmid containing a gene of interest plus the amp^R gene is used to transform E. coli cells. When these cells are placed on a medium containing ampicillin, what will happen?