Question 1

In eukaryotes how many polymerases catalyze transcription?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4 C

Question 2

Which type of gene therapy is used in clinical trials to treat cystic fibrosis?

Accepted Answer

A)  in vitro 
B)  in vivo 
C)  ex vitro 
D)  ex vivo 
A)  in vitro 
B)  in vivo 
C)  ex vitro 
D)  ex vivo B

Question 3

After an amino acid has formed a peptide bond, where is its tRNA translocated to?

Accepted Answer

A)  the A site 
B)  the E site 
C)  the P site 
D)  the T site 
A)  the A site 
B)  the E site 
C)  the P site 
D)  the T site C

Question 4

Which position of a codon is generally the least important in determining which amino acid the codon codes for?

Accepted Answer

A)  the first 
B)  the second 
C)  the third 
D)  None of them, they are all equally important. 
A)  the first 
B)  the second 
C)  the third 
D)  None of them, they are all equally important.

Question 5

In animal experiments overexpression of p53 resulted in which of the following?

Accepted Answer

A)  acceleration of aging 
B)  prevention of cancer 
C)  both a and b 
D)  neither a nor b 
A)  acceleration of aging 
B)  prevention of cancer 
C)  both a and b 
D)  neither a nor b

Question 6

Where on the tRNA does the amino acid bind?

Accepted Answer

A)  at the 3' end 
B)  at the 5' end 
C)  at the anticodon loop 
D)  at a methionine residue 
A)  at the 3' end 
B)  at the 5' end 
C)  at the anticodon loop 
D)  at a methionine residue

Question 7

The genetic code is associated with which of the following processes?

Accepted Answer

A)  DNA replication 
B)  translation 
C)  transcription 
D)  all of these 
A)  DNA replication 
B)  translation 
C)  transcription 
D)  all of these

Question 8

Which of the following is not found in transcription factors?

Accepted Answer

A)  helix-turn-helix 
B)  leucine zipper 
C)  zinc finger 
D)  None, they are all found in transcription factors. 
A)  helix-turn-helix 
B)  leucine zipper 
C)  zinc finger 
D)  None, they are all found in transcription factors.

Question 9

How many sticky ends are produced if a certain restriction endonuclease cleaves a plasmid at three different locations ?

Accepted Answer

A)  3 
B)  6 
C)  8 
D)  9 
A)  3 
B)  6 
C)  8 
D)  9

Question 10

HIV, the retrovirus which causes AIDS, does which of the following?

Accepted Answer

A)  uses reverse transcriptase to synthesize DNA 
B)  uses RNA replicase replicate it own RNA 
C)  both a and b 
D)  neither a nor b 
A)  uses reverse transcriptase to synthesize DNA 
B)  uses RNA replicase replicate it own RNA 
C)  both a and b 
D)  neither a nor b

Question 11

Which of the following is carried by tRNAfMet during the formation of the pre-initiation complex?

Accepted Answer

A)  flavinmethionine 
B)  fluoromethionine 
C)  formylmethionine 
D)  fullermethionine 
A)  flavinmethionine 
B)  fluoromethionine 
C)  formylmethionine 
D)  fullermethionine

Question 12

Which of the following is involved in ensuring that the small ribosomal unit is properly aligned on the mRNA

Accepted Answer

A)  the anticodon 
B)  the codon 
C)  the Shine-Dalgarno sequence 
D)  the Svedberg sequence 
A)  the anticodon 
B)  the codon 
C)  the Shine-Dalgarno sequence 
D)  the Svedberg sequence

Question 13

Which of the following is true of the relationship between amino acids, tRNAs and aminoacyl-tRNA synthetases?

Accepted Answer

A)  All amino acids have one tRNA and one aminoacyl-tRNA synthetase. 
B)  All amino acids have more than one tRNA and each tRNA has its own aminoacyl-tRNA synthetase. 
C)  Some amino acids have more than one tRNA, but these have only one aminoacyl-tRNA synthetase. 
D)  Some amino acids have more than one tRNA and each tRNA has its own aminoacyl-tRNA synthetase. 
A)  All amino acids have one tRNA and one aminoacyl-tRNA synthetase. 
B)  All amino acids have more than one tRNA and each tRNA has its own aminoacyl-tRNA synthetase. 
C)  Some amino acids have more than one tRNA, but these have only one aminoacyl-tRNA synthetase. 
D)  Some amino acids have more than one tRNA and each tRNA has its own aminoacyl-tRNA synthetase.

Question 14

How does an enhancer stimulate transcription?

Accepted Answer

A)  It brings more nucleotides to the site transcription site. 
B)  It brings more tRNA to the transcription site. 
C)  It binds to regions of DNA far removed from the transcription site and then binds to the transcription site. 
D)  None of these is correct. 
A)  It brings more nucleotides to the site transcription site. 
B)  It brings more tRNA to the transcription site. 
C)  It binds to regions of DNA far removed from the transcription site and then binds to the transcription site. 
D)  None of these is correct.

Question 15

What type of bond is formed when an amino acid binds to its tRNA?

Accepted Answer

A)  an amide bond 
B)  an ester bond 
C)  an ionic bond 
D)  The type of bond depends on the specific amino acid. 
A)  an amide bond 
B)  an ester bond 
C)  an ionic bond 
D)  The type of bond depends on the specific amino acid.

Question 16

In which type of gene therapy is the patient given the virus directly?

Accepted Answer

A)  in vitro 
B)  in vivo 
C)  ex vitro 
D)  ex vivo 
A)  in vitro 
B)  in vivo 
C)  ex vitro 
D)  ex vivo

Question 17

Which amino acids are found at the C-terminal domain of pol II?

Accepted Answer

A)  alanine and serine 
B)  alanine and glycine 
C)  glycine and threonine 
D)  serine and threonine 
A)  alanine and serine 
B)  alanine and glycine 
C)  glycine and threonine 
D)  serine and threonine

Question 18

How many sites are associated with the full ribosomal complex?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 19

The following generalized reaction is used in one of the techniques for synthesizing recombinant DNA.   What type of enzyme is needed for reaction?

Accepted Answer

A)  DNA ligase 
B)  restriction endonuclease 
C)  peptidyl transferase 
D)  DNA synthetase 
A)  DNA ligase 
B)  restriction endonuclease 
C)  peptidyl transferase 
D)  DNA synthetase

Question 20

How many codons, including redundant codons, would there be if proteins involved 100 different amino acids rather than 20?

Accepted Answer

A)  4 
B)  128 
C)  256 
D)  512 
A)  4 
B)  128 
C)  256 
D)  512

Exam 26: Gene Expression and Protein Synthesis

In eukaryotes how many polymerases catalyze transcription?

Which type of gene therapy is used in clinical trials to treat cystic fibrosis?

After an amino acid has formed a peptide bond, where is its tRNA translocated to?

Which position of a codon is generally the least important in determining which amino acid the codon codes for?

In animal experiments overexpression of p53 resulted in which of the following?

Where on the tRNA does the amino acid bind?

The genetic code is associated with which of the following processes?

Which of the following is not found in transcription factors?

How many sticky ends are produced if a certain restriction endonuclease cleaves a plasmid at three different locations ?

HIV, the retrovirus which causes AIDS, does which of the following?

Which of the following is carried by tRNAfMet during the formation of the pre-initiation complex?

Which of the following is involved in ensuring that the small ribosomal unit is properly aligned on the mRNA

Which of the following is true of the relationship between amino acids, tRNAs and aminoacyl-tRNA synthetases?

How does an enhancer stimulate transcription?

What type of bond is formed when an amino acid binds to its tRNA?

In which type of gene therapy is the patient given the virus directly?

Which amino acids are found at the C-terminal domain of pol II?

How many sites are associated with the full ribosomal complex?

The following generalized reaction is used in one of the techniques for synthesizing recombinant DNA. What type of enzyme is needed for reaction?

How many codons, including redundant codons, would there be if proteins involved 100 different amino acids rather than 20?

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Which of the following is carried by tRNA^fMet during the formation of the pre-initiation complex?