Question 1

Serial dilution can be used in combination with pour plates as a method for isolating pure cultures.

Accepted Answer

A) True 
 B)False

Question 2

A laboratory recipe lists sucrose, an ammonia compound, calcium salts and a buffer as the ingredients for a microbiologic medium. These are added to water and autoclaved. What type of medium does this protocol produce?

Accepted Answer

A)  defined agar 
B)  defined broth 
C)  complex broth 
D)  reducing agar 
E)  complex enrichment broth 
A)  defined agar 
B)  defined broth 
C)  complex broth 
D)  reducing agar 
E)  complex enrichment broth

Question 3

A microorganism found living under conditions of high ________ is a barophile.

Accepted Answer

A)  pH values 
B)  oxygen concentrations 
C)  hydrostatic pressure 
D)  carbon dioxide levels 
E)  salt concentrations 
A)  pH values 
B)  oxygen concentrations 
C)  hydrostatic pressure 
D)  carbon dioxide levels 
E)  salt concentrations

Question 4

Reducing medium is used to isolate ________ microbes.

Accepted Answer

A)  fastidious 
B)  obligate aerobic 
C)  autotrophic 
D)  obligate anaerobic 
E)  intracellular parasitic 
A)  fastidious 
B)  obligate aerobic 
C)  autotrophic 
D)  obligate anaerobic 
E)  intracellular parasitic

Question 5

The carotenoid pigments of some phototrophs interact with ________ to reduce its toxicity.

Accepted Answer

A)  singlet oxygen (¹O₂) 
B)  hydroxyl radical (HO⁻) 
C)  peroxide anion (O₂²⁻) 
D)  superoxide radical (O₂⁻) 
E)  molecular oxygen (O₂) 
A)  singlet oxygen (¹O₂) 
B)  hydroxyl radical (HO⁻) 
C)  peroxide anion (O₂²⁻) 
D)  superoxide radical (O₂⁻) 
E)  molecular oxygen (O₂)

Question 6

A clinical sample from a mucus membrane is usually collected as a (biopsy/needle/swab) specimen.

Accepted Answer

The answer of A clinical sample from a mucus membrane...

Question 7

Explain the similarities and differences between direct and indirect methods of measuring microbial growth, and give examples of each.

Accepted Answer

Direct methods of measuring microbial gr

Question 8

Culturing of intracellular parasites generally requires (enrichment/cell culture/low oxygen) conditions.

Accepted Answer

The answer of Culturing of intracellular parasites generally requires (enrichment/cell...

Question 9

A new prokaryote is recovered from a remote saline hot spring. Design a set of experiments to determine whether the organism is thermophilic or thermoduric, halophilic or halotolerant.

Accepted Answer

The experimental design should include a

Question 10

Which of the following statements is CORRECT regarding Figure 6.1?

Accepted Answer

A)  Cells are actively growing only during the stage represented by B. 
B)  No cells are reproducing during the stage represented by D. 
C)  Cells are most susceptible to antibiotics during the stage represented by A. 
D)  Cells are alive but not reproducing during the stage represented by A. 
E)  No new cells are being produced during the stage represented by C. 
A)  Cells are actively growing only during the stage represented by B. 
B)  No cells are reproducing during the stage represented by D. 
C)  Cells are most susceptible to antibiotics during the stage represented by A. 
D)  Cells are alive but not reproducing during the stage represented by A. 
E)  No new cells are being produced during the stage represented by C.

Question 11

Rates of cell production and cell death are approximately equal during the ________ phase of growth.

Accepted Answer

A)  lag 
B)  log 
C)  death 
D)  stationary 
E)  intermediate 
A)  lag 
B)  log 
C)  death 
D)  stationary 
E)  intermediate

Question 12

Flow cytometry makes use of fluorescence to count microbes.

Accepted Answer

A) True 
 B)False

Question 13

Organisms that can grow with or without oxygen present are

Accepted Answer

A)  obligate aerobes. 
B)  facultative anaerobes. 
C)  aerotolerant anaerobes. 
D)  obligate anaerobes. 
E)  either facultative anaerobes or aerotolerant anaerobes. 
A)  obligate aerobes. 
B)  facultative anaerobes. 
C)  aerotolerant anaerobes. 
D)  obligate anaerobes. 
E)  either facultative anaerobes or aerotolerant anaerobes.

Question 14

Explain how temperature and pH levels can influence microbial infections in the human body.

Accepted Answer

All microbes have particular ranges of t

Question 15

Photoheterotrophs use carbon dioxide as their sole carbon source.

Accepted Answer

A) True 
 B)False

Question 16

Streak plates are a useful way to obtain CFUs.

Accepted Answer

A) True 
 B)False

Question 17

The preferred method for long term storage of bacteria is (freezing/refrigeration/lyophilization).

Accepted Answer

The answer of The preferred method for long term storage...

Question 18

In the process of ________, microbes detect the presence and density of other microbes and modify their metabolic activity in response.

Accepted Answer

A)  quorum sensing 
B)  antagonism 
C)  mutualism 
D)  symbiosis 
E)  synergy 
A)  quorum sensing 
B)  antagonism 
C)  mutualism 
D)  symbiosis 
E)  synergy

Question 19

Joan wants to discover a microbe capable of degrading an environmental contaminant. Which of the following culturing methods would be most effective for isolation of such an organism?

Accepted Answer

A)  cell culture 
B)  chemostat 
C)  quorum sensing 
D)  enrichment culture 
E)  differential culture 
A)  cell culture 
B)  chemostat 
C)  quorum sensing 
D)  enrichment culture 
E)  differential culture

Question 20

Thermoduric mesophiles are often responsible for spoilage of improperly canned foods.

Accepted Answer

A) True 
 B)False

Exam 6: Microbial Nutrition and Growth

Serial dilution can be used in combination with pour plates as a method for isolating pure cultures.

A laboratory recipe lists sucrose, an ammonia compound, calcium salts and a buffer as the ingredients for a microbiologic medium. These are added to water and autoclaved. What type of medium does this protocol produce?

A microorganism found living under conditions of high ________ is a barophile.

Reducing medium is used to isolate ________ microbes.

The carotenoid pigments of some phototrophs interact with ________ to reduce its toxicity.

A clinical sample from a mucus membrane is usually collected as a (biopsy/needle/swab) specimen.

Explain the similarities and differences between direct and indirect methods of measuring microbial growth, and give examples of each.

Culturing of intracellular parasites generally requires (enrichment/cell culture/low oxygen) conditions.

A new prokaryote is recovered from a remote saline hot spring. Design a set of experiments to determine whether the organism is thermophilic or thermoduric, halophilic or halotolerant.

Which of the following statements is CORRECT regarding Figure 6.1?

Rates of cell production and cell death are approximately equal during the ________ phase of growth.

Flow cytometry makes use of fluorescence to count microbes.

Organisms that can grow with or without oxygen present are

Explain how temperature and pH levels can influence microbial infections in the human body.

Photoheterotrophs use carbon dioxide as their sole carbon source.

Streak plates are a useful way to obtain CFUs.

The preferred method for long term storage of bacteria is (freezing/refrigeration/lyophilization).

In the process of ________, microbes detect the presence and density of other microbes and modify their metabolic activity in response.

Joan wants to discover a microbe capable of degrading an environmental contaminant. Which of the following culturing methods would be most effective for isolation of such an organism?

Thermoduric mesophiles are often responsible for spoilage of improperly canned foods.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Genetics

Recombinant DNA Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Industrial Microbiology

Microbial Ecology and Microbiomes

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