Question 1

Quorum sensing is involved in the development and propagation of a biofilm.

Accepted Answer

A) True 
 B)False  True

Question 2

The ________ method provides an estimate of the CFUs (colony-forming units) in a sample.

Accepted Answer

A)  Coulter-counter 
B)  viable plate-count 
C)  streak-plate 
D)  turbidity 
E)  direct microscopic-count 
A)  Coulter-counter 
B)  viable plate-count 
C)  streak-plate 
D)  turbidity 
E)  direct microscopic-count B

Question 3

A sample placed into fresh medium is initially in the (lag/log/stationary) phase of microbial growth in most instances.

Accepted Answer

lag

Question 4

A (Coulter/Petroff-Hauser chamber/spectrophotometer) counter is used to count cells on a light microscope.

Accepted Answer

Petroff-Ha

Question 5

A microbe that grows only at the bottom of a tube of thioglycollate medium is probably a(n)

Accepted Answer

A)  obligate aerobe. 
B)  facultative anaerobe. 
C)  aerotolerant anaerobe. 
D)  microaerophile. 
E)  obligate anaerobe. 
A)  obligate aerobe. 
B)  facultative anaerobe. 
C)  aerotolerant anaerobe. 
D)  microaerophile. 
E)  obligate anaerobe.

Question 6

The ________ of a population is the time it takes for the cells to double in number.

Accepted Answer

A)  binary fission 
B)  exponential growth 
C)  generation time 
D)  arithmetic growth 
E)  log phase 
A)  binary fission 
B)  exponential growth 
C)  generation time 
D)  arithmetic growth 
E)  log phase

Question 7

Nitrogen is a growth-limiting nutrient for many organisms because

Accepted Answer

A)  it is necessary for the biosynthesis of amino acids. 
B)  it is required for synthesis of nucleotides. 
C)  it is required for lipid synthesis. 
D)  only a small number of bacteria are able to extract it from the atmosphere. 
E)  only a few microbes can extract it from the atmosphere, but all organisms require it for amino acid and nucleotide synthesis. 
A)  it is necessary for the biosynthesis of amino acids. 
B)  it is required for synthesis of nucleotides. 
C)  it is required for lipid synthesis. 
D)  only a small number of bacteria are able to extract it from the atmosphere. 
E)  only a few microbes can extract it from the atmosphere, but all organisms require it for amino acid and nucleotide synthesis.

Question 8

Blood agar plates are an example of ________ medium.

Accepted Answer

A)  differential 
B)  selective 
C)  reducing 
D)  defined 
E)  transport 
A)  differential 
B)  selective 
C)  reducing 
D)  defined 
E)  transport

Question 9

The Most Probable Number (MPN) method is used

Accepted Answer

A)  to estimate numbers of cells in samples with a high density of bacterial cells. 
B)  when samples contain obligate anaerobes. 
C)  to estimate numbers of cells in samples with a low density of bacterial cells. 
D)  to isolate autotrophic bacteria. 
E)  to determine the generation time of bacterial isolates. 
A)  to estimate numbers of cells in samples with a high density of bacterial cells. 
B)  when samples contain obligate anaerobes. 
C)  to estimate numbers of cells in samples with a low density of bacterial cells. 
D)  to isolate autotrophic bacteria. 
E)  to determine the generation time of bacterial isolates.

Question 10

A ________ is used in industrial microbiology to produce microbial products that are only synthesized during log phase growth.

Accepted Answer

A)  Coulter counter 
B)  broth culture 
C)  spectrophotometer 
D)  pour plate 
E)  chemostat 
A)  Coulter counter 
B)  broth culture 
C)  spectrophotometer 
D)  pour plate 
E)  chemostat

Question 11

Some organisms use vitamins as (oxidizers/antioxidants/peroxidases) to be able to live as aerobes.

Accepted Answer

The answer of Some organisms use vitamins as (oxidizers/antioxidants/peroxidases) to...

Question 12

A capnophile is a microorganism that thrives in conditions of high

Accepted Answer

A)  carbon dioxide levels. 
B)  hydrostatic pressure. 
C)  oxygen levels. 
D)  pH. 
E)  salt concentration. 
A)  carbon dioxide levels. 
B)  hydrostatic pressure. 
C)  oxygen levels. 
D)  pH. 
E)  salt concentration.

Question 13

Creating conditions in the laboratory that promote the growth of some microbes while inhibiting the growth of others is called

Accepted Answer

A)  complex culturing. 
B)  reducing conditions. 
C)  enrichment culturing. 
D)  transport conditions. 
E)  differential culturing. 
A)  complex culturing. 
B)  reducing conditions. 
C)  enrichment culturing. 
D)  transport conditions. 
E)  differential culturing.

Question 14

At temperatures higher than the maximum growth temperature for an organism

Accepted Answer

A)  proteins are permanently denatured. 
B)  membranes become too fluid for proper function. 
C)  hydrogen bonds within molecules are broken. 
D)  hydrogen bonds are broken and proteins are permanently denatured. 
E)  hydrogen bonds are broken, proteins are denatured, and membranes become too fluid. 
A)  proteins are permanently denatured. 
B)  membranes become too fluid for proper function. 
C)  hydrogen bonds within molecules are broken. 
D)  hydrogen bonds are broken and proteins are permanently denatured. 
E)  hydrogen bonds are broken, proteins are denatured, and membranes become too fluid.

Question 15

A(n) (culture/specimen/inoculum) is a sample of microorganisms introduced into a growth medium.

Accepted Answer

The answer of A(n) (culture/specimen/inoculum) is a sample of microorganisms...

Question 16

The (peroxide/superoxide/hydroxyl) radical is formed during the incomplete reduction of O₂ during electron transport in aerobes.

Accepted Answer

The answer of The (peroxide/superoxide/hydroxyl) radical is formed during the...

Question 17

Which of the following is an indirect method for estimating the number of microbes in a sample?

Accepted Answer

A)  viable plate counts 
B)  turbidity 
C)  Coulter counter 
D)  MPN 
E)  membrane filtration 
A)  viable plate counts 
B)  turbidity 
C)  Coulter counter 
D)  MPN 
E)  membrane filtration

Question 18

A clinical sample labeled as "sputum" was collected from

Accepted Answer

A)  the skin. 
B)  the blood. 
C)  the central nervous system. 
D)  a piece of tissue. 
E)  the lungs. 
A)  the skin. 
B)  the blood. 
C)  the central nervous system. 
D)  a piece of tissue. 
E)  the lungs.

Question 19

The absence of ________ leaves obligate anaerobes susceptible to killing by oxygen.

Accepted Answer

A)  carotenoids 
B)  superoxide dismutase 
C)  peroxidase 
D)  vitamin E 
E)  catalase 
A)  carotenoids 
B)  superoxide dismutase 
C)  peroxidase 
D)  vitamin E 
E)  catalase

Question 20

________ are complex communities of various types of microbes that adhere to surfaces.

Accepted Answer

A)  Aggregates 
B)  Colonies 
C)  Isolates 
D)  Biofilms 
E)  Media 
A)  Aggregates 
B)  Colonies 
C)  Isolates 
D)  Biofilms 
E)  Media

Exam 6: Microbial Nutrition and Growth

Quorum sensing is involved in the development and propagation of a biofilm.

The ________ method provides an estimate of the CFUs (colony-forming units) in a sample.

A sample placed into fresh medium is initially in the (lag/log/stationary) phase of microbial growth in most instances.

A (Coulter/Petroff-Hauser chamber/spectrophotometer) counter is used to count cells on a light microscope.

A microbe that grows only at the bottom of a tube of thioglycollate medium is probably a(n)

The ________ of a population is the time it takes for the cells to double in number.

Nitrogen is a growth-limiting nutrient for many organisms because

Blood agar plates are an example of ________ medium.

The Most Probable Number (MPN) method is used

A ________ is used in industrial microbiology to produce microbial products that are only synthesized during log phase growth.

Some organisms use vitamins as (oxidizers/antioxidants/peroxidases) to be able to live as aerobes.

A capnophile is a microorganism that thrives in conditions of high

Creating conditions in the laboratory that promote the growth of some microbes while inhibiting the growth of others is called

At temperatures higher than the maximum growth temperature for an organism

A(n) (culture/specimen/inoculum) is a sample of microorganisms introduced into a growth medium.

The (peroxide/superoxide/hydroxyl) radical is formed during the incomplete reduction of O₂ during electron transport in aerobes.

Which of the following is an indirect method for estimating the number of microbes in a sample?

A clinical sample labeled as "sputum" was collected from

The absence of ________ leaves obligate anaerobes susceptible to killing by oxygen.

________ are complex communities of various types of microbes that adhere to surfaces.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Genetics

Recombinant DNA Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Industrial Microbiology

Microbial Ecology and Microbiomes

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