Exam 11: Genetic Engineering

mRNA transcripts are converted into cDNA by

M13 is an ssDNA filamentous phage used for

Describe the three main steps to clone a gene into an organism.

When is it appropriate to use an artificial chromosome vector? Describe a specific example.

Although various codons often code for the same amino acid, it is important to choose the codon preferred by the expression host itself.

When trying to express a mammalian gene in a bacterium, expression vectors are often used. Using your knowledge of prokaryotic and eukaryotic genetics, explain why expression vectors are so important.

Which of the following genomes have almost no introns?

Codon bias offers clues about reading frame functionality because most organisms show preferences among_________ .

Expression vectors used for cloning bacterial genes into eukaryotic cells must contain a(n)_________that functions efficiently in the host and one that is correctly positioned to drive transcription of the cloned gene.

Which of the following sequences is a palindrome?

Detecting a specific protein with an antibody is considered a(n) _________method.

If a foreign gene is cloned into a host, it is important that the cloned host itself

A cloning vector that can stably replicate in two unrelated host organisms is known as a(n)_________.

Which of the following procedures was named after its developer?

Describe the procedure used for colony hybridization and why it is useful.

One important advantage of eukaryotic cells as hosts for cloning vectors is that they already possess the complex RNA and posttranslational processing systems required for the production of eukaryotic proteins.

Restriction enzymes are widespread among_________ , where they serve a protective function.

Type II restriction endonucleases

After digesting a DNA sequence, a restriction endonuclease can generate

One limitation to designing an antibody for detecting a particular protein is that the antibody must be_________ for the protein of interest.