Exam 16: Microbial Evolution and Systematics

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Explain why recA, the gene encoding recombinase enzyme in Bacteria, is not used in evolutionary studies.

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The recA gene has a highly conserved nucleotide sequence. Because its nucleotide sequence is very similar throughout Bacteria, there are not enough differences to merit it a useful phylogenetic marker.

In doing comparative SSU rRNA sequencing, it is essential that pure (axenic) cultures be used.

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A monophyletic group is a group that

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D

Explain why in both taxonomy and phylogeny the reliance on the sequence of a single gene can have pitfalls.

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As oxygen appeared in the atmosphere, some of it accumulated in the form of________ , which formed a protective barrier that prevents________ from reaching Earth.

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What two gases were most abundant on early Earth?

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The domains arose as barriers between evolved organisms.

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One widely used cladistic method of examining evolutionary relationships is________ , which is based on the assumption that evolution is most likely to have proceeded by the path requiring fewest changes.

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T- RFLP is a molecular technique often applied for phylogenetic analyses of microbial communities.

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Hybridization values of 50% or greater are recommended for considering two isolates to be of the same species.

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Fossilized microbial mats consisting of layers of filamentous prokaryotes and trapped sediment are known as________ .

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Describe the hydrogen hypothesis and why it is favored over other hypotheses.

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Oxygen did not accumulate in the early atmosphere until it reacted with reduced materials, especially________ , in the oceans. These substances then reacted spontaneously with oxygen to produce________.

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RDP- II is the standard abbreviation of _________ .

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None of the organisms living today are primitive.

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Using present terminology, how many bacterial phyla have been discovered thus far?

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Explain how gene duplication can lead to new functions in haploids.

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The most widely used molecule in sequence- based evolutionary analyses are________ genes.

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Evolution is driven by

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Compared with today, the temperature on Earth during its first half- billion years was probably

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