Question 1

Molecular cloning includes

Accepted Answer

A)  locating the gene of interest. 
B)  placing the gene of interest into a convenient vector. 
C)  obtaining and purifying a copy of the gene of interest. 
D)  all of the above. 
A)  locating the gene of interest. 
B)  placing the gene of interest into a convenient vector. 
C)  obtaining and purifying a copy of the gene of interest. 
D)  all of the above.

Question 2

Explain why DNA fragments migrate toward the positive electrode and why some fragments migrate more rapidly than others during gel electrophoresis.

Accepted Answer

DNA is negatively charged due to its pho

Question 3

A(n)_________ mutation is one in which a DNA cassette is inserted at a restriction site in a cloned gene; transformed cells lose the function of the gene into which the cassette is inserted.

Accepted Answer

The answer of A(n)_________ mutation is one in which a...

Question 4

Although a gene library may contain tens of thousands of clones, finding the clone of interest is the biggest challenge.

Accepted Answer

A) True 
 B)False

Question 5

Artificial chromosomes are vectors capable of carrying_________ .

Accepted Answer

very large

Question 6

A vector that can be used to clone a desired gene that contains regulatory sequences so the expression of the gene can be manipulated is called a(n)_________ vector.

Accepted Answer

A)  stability 
B)  expression 
C)  endogenous 
D)  regulatory 
A)  stability 
B)  expression 
C)  endogenous 
D)  regulatory

Question 7

After DNA has been electrophoresed through a(n)_________ , a stain such as ethidium bromide is used to visualize different DNA sequence .

Accepted Answer

agarose ge

Question 8

Nucleic acid hybridization is widely used for _________segments of DNA.

Accepted Answer

A)  identifying 
B)  characterizing 
C)  detecting 
D)  all of the above. 
A)  identifying 
B)  characterizing 
C)  detecting 
D)  all of the above.

Question 9

The genes encoding luciferase, green fluorescent protein (GFP), and fi- galactosidase are typically used in cloning as

Accepted Answer

A)  global control genes. 
B)  reporter genes. 
C)  transcription regulators. 
D)  promoter sequences. 
A)  global control genes. 
B)  reporter genes. 
C)  transcription regulators. 
D)  promoter sequences.

Question 10

The enzyme that covalently links both strands of a vector and inserted DNA in molecular cloning is

Accepted Answer

A)  DNA ligase. 
B)  DNA phosphatase. 
C)  DNA transferase. 
D)  DNA hydrolase. 
A)  DNA ligase. 
B)  DNA phosphatase. 
C)  DNA transferase. 
D)  DNA hydrolase.

Question 11

By definition, genetic engineering techniques are performed

Accepted Answer

A)  in toto. 
B)  in vivo. 
C)  in situ. 
D)  in vitro. 
A)  in toto. 
B)  in vivo. 
C)  in situ. 
D)  in vitro.

Question 12

Explain how T7 promoters regulate transcription and why cloning vectors contain them.

Accepted Answer

The bacteriophage T7 polymerase gene is

Question 13

An illustration that indicates all regions where a particular restriction endonuclease cleaves a DNA sequence is called a(n) _________map.

Accepted Answer

A)  shotgun 
B)  reporter 
C)  restriction 
D)  cleavage 
A)  shotgun 
B)  reporter 
C)  restriction 
D)  cleavage

Question 14

The class of restriction endonucleases that cleave the DNA within their recognition sequences is known as

Accepted Answer

A)  type III. 
B)  type II. 
C)  type I. 
D)  All restriction endonucleases cut within their recognition sequence. 
A)  type III. 
B)  type II. 
C)  type I. 
D)  All restriction endonucleases cut within their recognition sequence.

Question 15

Site- directed mutagenesis is an in vitro method that removes mutations at specific locations in a sequence.

Accepted Answer

A) True 
 B)False

Question 16

Which statement is TRUE?

Accepted Answer

A)  YACs are more likely than BACs to undergo recombination and rearrangement. 
B)  YACs and BACs undergo recombination and rearrangement at about the same rate. 
C)  BACs are more likely than YACs to undergo recombination and rearrangement. 
D)  It is impossible to state with any certainty whether YACs or BACs are more likely to undergo recombination and rearrangement, because environmental factors play a major role in the probability of one or the other occurring. 
A)  YACs are more likely than BACs to undergo recombination and rearrangement. 
B)  YACs and BACs undergo recombination and rearrangement at about the same rate. 
C)  BACs are more likely than YACs to undergo recombination and rearrangement. 
D)  It is impossible to state with any certainty whether YACs or BACs are more likely to undergo recombination and rearrangement, because environmental factors play a major role in the probability of one or the other occurring.

Question 17

Explain how a DNA library is created.

Accepted Answer

The answer of Explain how a DNA library is created....

Question 18

Explain the function and usefulness of a polylinker within a vector.

Accepted Answer

A polylinker, also called a polyclonal s

Question 19

Recombinant vaccines have been developed using_________ .

Accepted Answer

The answer of Recombinant vaccines have been developed using_________ ....

Question 20

A major disadvantage of using Bacillus subtilis as a cloning host is that over several generations of subculturing,_________ .

Accepted Answer

the clonin

Exam 11: Genetic Engineering

Molecular cloning includes

Explain why DNA fragments migrate toward the positive electrode and why some fragments migrate more rapidly than others during gel electrophoresis.

A(n)_________ mutation is one in which a DNA cassette is inserted at a restriction site in a cloned gene; transformed cells lose the function of the gene into which the cassette is inserted.

Although a gene library may contain tens of thousands of clones, finding the clone of interest is the biggest challenge.

Artificial chromosomes are vectors capable of carrying_________ .

A vector that can be used to clone a desired gene that contains regulatory sequences so the expression of the gene can be manipulated is called a(n)_________ vector.

After DNA has been electrophoresed through a(n)_________ , a stain such as ethidium bromide is used to visualize different DNA sequence .

Nucleic acid hybridization is widely used for _________segments of DNA.

The genes encoding luciferase, green fluorescent protein (GFP), and fi- galactosidase are typically used in cloning as

The enzyme that covalently links both strands of a vector and inserted DNA in molecular cloning is

By definition, genetic engineering techniques are performed

Explain how T7 promoters regulate transcription and why cloning vectors contain them.

An illustration that indicates all regions where a particular restriction endonuclease cleaves a DNA sequence is called a(n) _________map.

The class of restriction endonucleases that cleave the DNA within their recognition sequences is known as

Site- directed mutagenesis is an in vitro method that removes mutations at specific locations in a sequence.

Which statement is TRUE?

Explain how a DNA library is created.

Explain the function and usefulness of a polylinker within a vector.

Recombinant vaccines have been developed using_________ .

A major disadvantage of using Bacillus subtilis as a cloning host is that over several generations of subculturing,_________ .

Microorganisms and Microbiology

A Brief Journey to the Microbial World

Cell Structure and Function in Bacteria and Archaea

Nutrition, Culture, and Metabolism of Microorganisms

Microbial Growth

Molecular Biology of Bacteria

Archael and Eukaryotic Molecular Biology

Regulation of Gene Expression

Viruses and Virology

Genetics of Bacteria and Arachaea

Microbial Genomics

Phototrophy, Chemolithotrophy, and Major Biosyntheses

Catabolism of Organic Compounds

Commercial Products and Biotechnology

Microbial Evolution and Systematics

Bacteria: the Proteobacteria

Other Bacteria

Archaea

Eukaryotic Cell Biology and Eukaryotic Microorganisms

Viral Diversity

Methods in Microbial Ecology

Major Microbial Habitats and Diversity

Nutrient Cycles, Biodegradation, and Bioremediation

Microbial Symbioses

Microbial Growth Control

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Diagnostic Microbiology and Immunology

Epidemiology

Person-To-Person Microbial Diseases

Vectorborne and Soilborne Microbial Pathogens

Wastewater Treatment, Water Purification, and Waterborne Microbial Diseases

Food Preservation and Foodborne Microbial Diseases

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