Exam 11: Genetic Engineering

Banding patterns generated from restriction analyses of either whole chromosomes or specific genes from a series of organisms can yield an indication of their genetic relationships.

One basic technique of genetic engineering includes the ability to cut the DNA of interest into random fragments.

Explain how foreign DNA of greater than 300 kbp can be inserted and stably maintained in BAC vectors.

Describe three characteristics of an ideal cloning host for genetic engineering.

Explain the process of microinjection.

Two problems of using Escherichia coli as a cloning host are_________ and_________ .

To make an adequate library for sequencing prokaryotic genes, a vector should hold inserts in the range of

Modification enzymes typically methylate specific bases within the recognition sequence to prevent digestion of the nucleotide sequence by restriction endonucleases.

Describe a method developed by molecular biologists to easily observe the success of a genetic engineering procedure involving the ligating of a gene of interest into a plasmid.

Explain the process of site- directed mutagenesis, and discuss some applications of this technique.

The principle behind a nucleic acid probe is that the probe itself contains

An integrating vector is a specific type of plasmid that is used for _________.

Explain how the process of cloning differs when a vector with sticky ends is used and when a vector with blunt ends is used.

The host for a BAC is typically a mutant strain of Escherichia coli that is missing_________ and is defective in __________.

Compare and contrast operon and protein fusions.

Cloning vectors are small, independently replicating genetic elements used to replicate cloned genes.

Explain how restriction sites, which are not random, can be a part of an approach to random cloning.

Expression vectors are designed to ensure that_________can be efficiently __________.

Retroviruses can be used to introduce foreign genes into mammalian cells, because_________.

Which statement is CORRECT about genomic analysis?