Question 1

EMB agar preferentially selects for the growth of _________bacteria.

Accepted Answer

A)  gram- negative 
B)  gram- variable 
C)  gram- positive 
D)  all of the above 
A)  gram- negative 
B)  gram- variable 
C)  gram- positive 
D)  all of the above

Question 2

Which organism can be detected in either humans or foods by nucleic acid probes?

Accepted Answer

A)  Chlamydia trachomatis 
B)  Staphylococcus aureus 
C)  Neisseria gonorrhoeae 
D)  all of the above 
A)  Chlamydia trachomatis 
B)  Staphylococcus aureus 
C)  Neisseria gonorrhoeae 
D)  all of the above

Question 3

The disk- diffusion test is used for

Accepted Answer

A)  minimum inhibitory concentration (MI
C)  determinations. 
B)  assessing antimicrobial activity. 
C)  serial dilution measurements. 
D)  all of the above. 
A)  minimum inhibitory concentration (MI
C)  determinations. 
B)  assessing antimicrobial activity. 
C)  serial dilution measurements. 
D)  all of the above.

Question 4

Two- component probes function as both a(n)_________ and a(n)_________ .

Accepted Answer

capture pr

Question 5

Two important intestinal pathogens generally acquired from contaminated food or water are_________ and ._________

Accepted Answer

Escherichia coli O15

Question 6

A gram- negative, oxidase- positive diplococcus growing on chocolate agar likely belongs to the genus _________.

Accepted Answer

The answer of A gram- negative, oxidase- positive diplococcus growing...

Question 7

Which bacterium shows a characteristic green sheen when grown on eosin- methylene blue (EM.B agar?

Accepted Answer

A)  Streptococcus pyogenes 
B)  Salmonella enterica 
C)  Staphylococcus aureus 
D)  Escherichia coli 
A)  Streptococcus pyogenes 
B)  Salmonella enterica 
C)  Staphylococcus aureus 
D)  Escherichia coli

Question 8

Bacteremia is a term used

Accepted Answer

A)  when the same organism is isolated from more than one site. 
B)  to indicate the presence of bacteria in the blood. 
C)  synonymously with septicemia. 
D)  in all of the above circumstances. 
A)  when the same organism is isolated from more than one site. 
B)  to indicate the presence of bacteria in the blood. 
C)  synonymously with septicemia. 
D)  in all of the above circumstances.

Question 9

An Etest is a clinical tool used for the determination of

Accepted Answer

A)  the minimum inhibitory concentration (MI
C)  value of an antimicrobial agent. 
B)  enteric presence. 
C)  pathogenic enzyme activity. 
D)  Escherichia coli O157:H7 presence. 
A)  the minimum inhibitory concentration (MI
C)  value of an antimicrobial agent. 
B)  enteric presence. 
C)  pathogenic enzyme activity. 
D)  Escherichia coli O157:H7 presence.

Question 10

In attempting to isolate a specimen from a disease site, recovery or detection may not be possible because of

Accepted Answer

A)  failure to meet the metabolic needs of the purported pathogen. 
B)  an insufficient inoculum. 
C)  failure to ensure aseptic conditions. 
D)  all of the above. 
A)  failure to meet the metabolic needs of the purported pathogen. 
B)  an insufficient inoculum. 
C)  failure to ensure aseptic conditions. 
D)  all of the above.

Question 11

Intestinal parasites can be identified by observing stool samples for the presence of

Accepted Answer

A)  LPS. 
B)  antibodies. 
C)  blood. 
D)  cysts. 
A)  LPS. 
B)  antibodies. 
C)  blood. 
D)  cysts.

Question 12

The dipstick nitrite test is a very rapid check for urinary tract infections because

Accepted Answer

A)  nitrite is reduced to nitrate in the presence of urine. 
B)  only pathogenic bacteria produce nitrite. 
C)  significant nitrite production is apparent only when large numbers of enteric bacteria are present. 
D)  of all of the above. 
A)  nitrite is reduced to nitrate in the presence of urine. 
B)  only pathogenic bacteria produce nitrite. 
C)  significant nitrite production is apparent only when large numbers of enteric bacteria are present. 
D)  of all of the above.

Question 13

In cases of suspected foodborne or waterborne infections, fecal samples should be inoculated into a variety of selective media.

Accepted Answer

A) True 
 B)False

Question 14

Compared with other precipitation tests, agglutination tests are

Accepted Answer

A)  less sensitive. 
B)  more sensitive. 
C)  highly variable in sensitivity, and thus the sensitivities cannot be compared. 
D)  about equally sensitive. 
A)  less sensitive. 
B)  more sensitive. 
C)  highly variable in sensitivity, and thus the sensitivities cannot be compared. 
D)  about equally sensitive.

Question 15

Factors rendering EIA a useful immunodiagnostic tool include

Accepted Answer

A)  sensitivity. 
B)  long shelf life. 
C)  speed. 
D)  all of the above. 
A)  sensitivity. 
B)  long shelf life. 
C)  speed. 
D)  all of the above.

Question 16

Literally hundreds of clinically useful EIAs have been developed.

Accepted Answer

A) True 
 B)False

Question 17

A laboratory in which Ebola virus is studied would be classified as a BSL- _________laboratory.

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4

Question 18

One advantage of the fluorescent antibodies that permits disease diagnosis long before primary isolation techniques yield results is that

Accepted Answer

A)  few controls are required. 
B)  cross- reactions do not occur. 
C)  it may be applied directly to tissue. 
D)  nonspecific staining does not occur. 
A)  few controls are required. 
B)  cross- reactions do not occur. 
C)  it may be applied directly to tissue. 
D)  nonspecific staining does not occur.

Question 19

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

Accepted Answer

Molecular biology techniques that genera

Question 20

When using the EIA test for HIV screening, both false negative and false positive results are possible.

Accepted Answer

A) True 
 B)False

Exam 31: Diagnostic Microbiology and Immunology

EMB agar preferentially selects for the growth of _________bacteria.

Which organism can be detected in either humans or foods by nucleic acid probes?

The disk- diffusion test is used for

Two- component probes function as both a(n)_________ and a(n)_________ .

Two important intestinal pathogens generally acquired from contaminated food or water are_________ and ._________

A gram- negative, oxidase- positive diplococcus growing on chocolate agar likely belongs to the genus _________.

Which bacterium shows a characteristic green sheen when grown on eosin- methylene blue (EM.B agar?

Bacteremia is a term used

An Etest is a clinical tool used for the determination of

In attempting to isolate a specimen from a disease site, recovery or detection may not be possible because of

Intestinal parasites can be identified by observing stool samples for the presence of

The dipstick nitrite test is a very rapid check for urinary tract infections because

In cases of suspected foodborne or waterborne infections, fecal samples should be inoculated into a variety of selective media.

Compared with other precipitation tests, agglutination tests are

Factors rendering EIA a useful immunodiagnostic tool include

Literally hundreds of clinically useful EIAs have been developed.

A laboratory in which Ebola virus is studied would be classified as a BSL- _________laboratory.

One advantage of the fluorescent antibodies that permits disease diagnosis long before primary isolation techniques yield results is that

Discuss how recent advances in the molecular biology of nucleic acids have revolutionized methods used for the identification of infectious disease agents and how they are favored over traditional biochemical tests.

When using the EIA test for HIV screening, both false negative and false positive results are possible.

Microorganisms and Microbiology

A Brief Journey to the Microbial World

Cell Structure and Function in Bacteria and Archaea

Nutrition, Culture, and Metabolism of Microorganisms

Microbial Growth

Molecular Biology of Bacteria

Archael and Eukaryotic Molecular Biology

Regulation of Gene Expression

Viruses and Virology

Genetics of Bacteria and Arachaea

Genetic Engineering

Microbial Genomics

Phototrophy, Chemolithotrophy, and Major Biosyntheses

Catabolism of Organic Compounds

Commercial Products and Biotechnology

Microbial Evolution and Systematics

Bacteria: the Proteobacteria

Other Bacteria

Archaea

Eukaryotic Cell Biology and Eukaryotic Microorganisms

Viral Diversity

Methods in Microbial Ecology

Major Microbial Habitats and Diversity

Nutrient Cycles, Biodegradation, and Bioremediation

Microbial Symbioses

Microbial Growth Control

Microbial Interactions With Humans

Immunity and Host Defense

Immune Mechanisms

Molecular Immunology

Epidemiology

Person-To-Person Microbial Diseases

Vectorborne and Soilborne Microbial Pathogens

Wastewater Treatment, Water Purification, and Waterborne Microbial Diseases

Food Preservation and Foodborne Microbial Diseases

Filters

Two- component probes function as both a(n)_ and a(n)_ .

Two important intestinal pathogens generally acquired from contaminated food or water are_ and ._