Question 1

How many potential codons does the following mRNA sequence contain? CUCUCUCUCUCUC

Accepted Answer

A)  0 
B)  1 
C)  2 
D)  3 
A)  0 
B)  1 
C)  2 
D)  3

Question 2

A common feature of translation in both eukaryotes and prokaryotes is

Accepted Answer

A)  hydrolysis of ATP to facilitate translocation of the ribosome. 
B)  hydrolysis of GTP to promote binding of the AA-tRNAAA. 
C)  a P site that can contain uncharged tRNA. 
D)  an E site that can contain a tRNA covalently bound to a nascent polypeptide. 
A)  hydrolysis of ATP to facilitate translocation of the ribosome. 
B)  hydrolysis of GTP to promote binding of the AA-tRNAAA. 
C)  a P site that can contain uncharged tRNA. 
D)  an E site that can contain a tRNA covalently bound to a nascent polypeptide.

Question 3

Consider an in vitro experiment in which all components needed for protein synthesis are present.If tRNAs charged with radioactively labeled amino acids are added,over time the radioactivity would be located in which of the following components?

Accepted Answer

A)  mRNA 
B)  aminoacyl tRNA synthetase 
C)  nascent protein 
D)  codons 
A)  mRNA 
B)  aminoacyl tRNA synthetase 
C)  nascent protein 
D)  codons

Question 4

Which of the following sequences is most likely a nuclear localization signal?

Accepted Answer

A)  Lys-Lys-Arg-Gly-Arg 
B)  Glu-Asp-Asp-Gly-Glu 
C)  Ile-Leu-Phe-Leu-Gly 
D)  Lys-Asp-Arg-Glu-Glu 
A)  Lys-Lys-Arg-Gly-Arg 
B)  Glu-Asp-Asp-Gly-Glu 
C)  Ile-Leu-Phe-Leu-Gly 
D)  Lys-Asp-Arg-Glu-Glu

Question 5

tRNA interacts with ribosomes at one of three sites: the E site,the P site,and the __________ site.

Accepted Answer

A)  T 
B)  M 
C)  SD 
D)  A 
A)  T 
B)  M 
C)  SD 
D)  A

Question 6

Where would an amino acid be attached to the tRNA below?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 7

If a protein is covalently modified by ubiquitin,it will be

Accepted Answer

A)  degraded. 
B)  secreted. 
C)  targeted to the nucleus. 
D)  anchored to the plasma membrane. 
A)  degraded. 
B)  secreted. 
C)  targeted to the nucleus. 
D)  anchored to the plasma membrane.

Question 8

Identify the antibiotic shown below and illustrate how it functions to inhibit bacterial cell growth.

Accepted Answer

This is streptomycin

Question 9

Which antibiotic functions by inhibiting cell wall synthesis?

Accepted Answer

A)  penicillin 
B)  linezolid 
C)  tetracycline 
D)  rifampin 
A)  penicillin 
B)  linezolid 
C)  tetracycline 
D)  rifampin

Question 10

How many GTP are hydrolyzed during a single round of translation elongation in a eukaryote?

Accepted Answer

A)  0 
B)  1 
C)  2 
D)  6 
A)  0 
B)  1 
C)  2 
D)  6

Question 11

Where might the molecule shown below be located in both the prokaryotic and eukaryotic ribosome?

Accepted Answer

A)  A site 
B)  E site 
C)  40S complex 
D)  70S complex 
A)  A site 
B)  E site 
C)  40S complex 
D)  70S complex

Question 12

If the following mRNA was added to a cell-free translation system,how many unique protein sequences would be generated? ACCACCACCACCACCACCACCACCACCACCACCACCACCACC

Accepted Answer

A)  14 
B)  7 
C)  2 
D)  3 
A)  14 
B)  7 
C)  2 
D)  3

Question 13

An experiment with bacteria that has been exposed to an antibiotic reveals that the translocation step has been inhibited.Which antibiotic was likely used?

Accepted Answer

A)  chloramphenicol 
B)  erythromycin 
C)  tetracycline 
D)  streptomycin 
A)  chloramphenicol 
B)  erythromycin 
C)  tetracycline 
D)  streptomycin

Question 14

Noncanonical base pairings of A and I would contain __________ hydrogen bonds.The base pairing of C and I would contain __________ hydrogen bonds.

Accepted Answer

A)  1; 3 
B)  1; 2 
C)  2; 2 
D)  2; 1 
A)  1; 3 
B)  1; 2 
C)  2; 2 
D)  2; 1

Question 15

At any time during nuclear import and export,Ran can be found associated with all EXCEPT

Accepted Answer

A)  GTP. 
B)  exportin. 
C)  GMP. 
D)  importin. 
A)  GTP. 
B)  exportin. 
C)  GMP. 
D)  importin.

Question 16

How does the wobble hypothesis allow for fewer than 63 tRNAs to effectively recognize 63 codons?

Accepted Answer

The wobble hypothesis states t

Question 17

Place the following steps in the elongation phase of translation in their proper order. 
A.Peptide bond formation occurs.
B.GTP is hydrolyzed,and the ribosome moves one codon in the   direction.
C.GTP is hydrolyzed,and EF-Tu•GDP is released.
D.tRNA is released from the E site.

Accepted Answer

A)  A; C; B; D 
B)  A; B; C; D 
C)  B; D; C; A 
D)  C; A; B; D 
A)  A; C; B; D 
B)  A; B; C; D 
C)  B; D; C; A 
D)  C; A; B; D

Question 18

A __________ is NOT a characteristic of the ER signal peptide sequence.

Accepted Answer

A)  N-terminal segment containing negatively charged amino acids 
B)  segment containing hydrophobic amino acids 
C)  C-terminal segment containing hydrophobic amino acids 
D)  protease cleavage site 
A)  N-terminal segment containing negatively charged amino acids 
B)  segment containing hydrophobic amino acids 
C)  C-terminal segment containing hydrophobic amino acids 
D)  protease cleavage site

Question 19

Which antibiotic functions by inhibiting DNA gyrase?

Accepted Answer

A)  vancomycin 
B)  coumermycin 
C)  aminoglycosides 
D)  clindamycin 
A)  vancomycin 
B)  coumermycin 
C)  aminoglycosides 
D)  clindamycin

Question 20

Differentiate between type I and type II aminoacyl-tRNA synthetases in how they interact with the tRNA and acceptor stem.

Accepted Answer

Class I aminoacyl-tRNA synthetases bind

Exam 22: Protein Synthesis, posttranslational Modification, and Transport

How many potential codons does the following mRNA sequence contain? CUCUCUCUCUCUC

A common feature of translation in both eukaryotes and prokaryotes is

Consider an in vitro experiment in which all components needed for protein synthesis are present.If tRNAs charged with radioactively labeled amino acids are added,over time the radioactivity would be located in which of the following components?

Which of the following sequences is most likely a nuclear localization signal?

tRNA interacts with ribosomes at one of three sites: the E site,the P site,and the __________ site.

Where would an amino acid be attached to the tRNA below?

If a protein is covalently modified by ubiquitin,it will be

Identify the antibiotic shown below and illustrate how it functions to inhibit bacterial cell growth.

Which antibiotic functions by inhibiting cell wall synthesis?

How many GTP are hydrolyzed during a single round of translation elongation in a eukaryote?

Where might the molecule shown below be located in both the prokaryotic and eukaryotic ribosome?

If the following mRNA was added to a cell-free translation system,how many unique protein sequences would be generated? ACCACCACCACCACCACCACCACCACCACCACCACCACCACC

An experiment with bacteria that has been exposed to an antibiotic reveals that the translocation step has been inhibited.Which antibiotic was likely used?

Noncanonical base pairings of A and I would contain __________ hydrogen bonds.The base pairing of C and I would contain __________ hydrogen bonds.

At any time during nuclear import and export,Ran can be found associated with all EXCEPT

How does the wobble hypothesis allow for fewer than 63 tRNAs to effectively recognize 63 codons?

Place the following steps in the elongation phase of translation in their proper order. A.Peptide bond formation occurs. B.GTP is hydrolyzed,and the ribosome moves one codon in the direction. C.GTP is hydrolyzed,and EF-Tu•GDP is released. D.tRNA is released from the E site.

A __________ is NOT a characteristic of the ER signal peptide sequence.

Which antibiotic functions by inhibiting DNA gyrase?

Differentiate between type I and type II aminoacyl-tRNA synthetases in how they interact with the tRNA and acceptor stem.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Gene Regulation

Filters

Noncanonical base pairings of A and I would contain hydrogen bonds.The base pairing of C and I would contain hydrogen bonds.