Question 1

Some bacteria grow at 25° C or 42° C,but diagnostic laboratories routinely grow pathogenic bacteria at what temperature?

Accepted Answer

A)  30° C 
B)  60° C 
C)  35° C 
D)  10° C 
A)  30° C 
B)  60° C 
C)  35° C 
D)  10° C

Question 2

Mycoplasma and Ureaplasma spp.must have media supplemented with serum or sugar as nutrients and because:

Accepted Answer

A)  Their cell walls contain only peptidoglycan. 
B)  They lack cell walls. 
C)  The sterols in their cell walls are soluble in normal bacterial media. 
D)  Their cell walls contain detoxifying enzymes. 
A)  Their cell walls contain only peptidoglycan. 
B)  They lack cell walls. 
C)  The sterols in their cell walls are soluble in normal bacterial media. 
D)  Their cell walls contain detoxifying enzymes.

Question 3

If bacteria utilize various carbohydrates for growth,it is usually detected by:

Accepted Answer

A)  Alkaline production and change of color from the pH indicator 
B)  Production of carbon dioxide 
C)  Production of keto acids 
D)  Acid production and change of color from the pH indicator 
A)  Alkaline production and change of color from the pH indicator 
B)  Production of carbon dioxide 
C)  Production of keto acids 
D)  Acid production and change of color from the pH indicator

Question 4

In what staining procedure does carbolfuchsin penetrate the bacterial cell wall through heat or detergent treatment?

Accepted Answer

A)  Gram stain 
B)  Acridine orange stain 
C)  Endospore stain 
D)  Acid-fast stain 
A)  Gram stain 
B)  Acridine orange stain 
C)  Endospore stain 
D)  Acid-fast stain

Question 5

When bacteria are growing,they go through a log phase when:

Accepted Answer

A)  They are preparing to divide. 
B)  Nutrients are becoming limited and the numbers of bacteria remain constant. 
C)  The number of nonviable bacterial cells exceeds the number of viable cells. 
D)  The bacteria numbers usually double with each generation time 
A)  They are preparing to divide. 
B)  Nutrients are becoming limited and the numbers of bacteria remain constant. 
C)  The number of nonviable bacterial cells exceeds the number of viable cells. 
D)  The bacteria numbers usually double with each generation time

Question 6

A microbiologist is working with two separate cultures of the same organism.The bacteria in one culture are resistant to penicillin,whereas the bacteria in the other culture are susceptible to penicillin.The bacteria from both cultures are mixed together,and all the resulting bacteria are resistant to penicillin.What caused this phenomenon?

Accepted Answer

A)  The plasmid carrying the resistance gene was transferred to the susceptible population of bacteria. 
B)  The plasmid carrying the susceptibility gene was transferred to the resistant population of bacteria. 
C)  An IS element was inserted into the genome of the susceptible bacterial population. 
D)  A frame-shift mutation occurred that allowed the susceptible population of bacteria to develop resistance to penicillin. 
A)  The plasmid carrying the resistance gene was transferred to the susceptible population of bacteria. 
B)  The plasmid carrying the susceptibility gene was transferred to the resistant population of bacteria. 
C)  An IS element was inserted into the genome of the susceptible bacterial population. 
D)  A frame-shift mutation occurred that allowed the susceptible population of bacteria to develop resistance to penicillin.

Question 7

A clinical laboratory scientist is working on the bench,reading plates,and notices that a culture has both a unicellular form and a filamentous form.What type of organism exhibits these forms?

Accepted Answer

A)  Virus 
B)  Fungi 
C)  Bacteria 
D)  Parasite 
A)  Virus 
B)  Fungi 
C)  Bacteria 
D)  Parasite

Question 8

The three regions of the lipopolysaccharide include all the following EXCEPT:

Accepted Answer

A)  Antigenic O-specific polysaccharide 
B)  Mycolic acid 
C)  Core polysaccharide 
D)  Endotoxin (inner lipid 
A)  
A)  Antigenic O-specific polysaccharide 
B)  Mycolic acid 
C)  Core polysaccharide 
D)  Endotoxin (inner lipid 
A)

Exam 1: Bacterial Cell Structure, physiology, Metabolism, and Genetics

Some bacteria grow at 25° C or 42° C,but diagnostic laboratories routinely grow pathogenic bacteria at what temperature?

Mycoplasma and Ureaplasma spp.must have media supplemented with serum or sugar as nutrients and because:

If bacteria utilize various carbohydrates for growth,it is usually detected by:

In what staining procedure does carbolfuchsin penetrate the bacterial cell wall through heat or detergent treatment?

When bacteria are growing,they go through a log phase when:

A clinical laboratory scientist is working on the bench,reading plates,and notices that a culture has both a unicellular form and a filamentous form.What type of organism exhibits these forms?

The three regions of the lipopolysaccharide include all the following EXCEPT:

Host-Parasite Interaction

Laboratory Role in Infection Control

Control of Microorganisms

Performance Improvement in the Microbiology Laboratory

Specimen Collection and Processing

Microscopic Examination of Infected Materials

Use of Colonial Morphology for the Presumptive Identification of Microorganisms

Biochemical Identification of Gram-Negative Bacteria

Immunodiagnosis of Infectious Diseases

Applications of Molecular Diagnostics

Antibiotic Mechanisms of Action and Resistance

Antimicrobial Susceptibility Testing

Staphylococci

Streptococcus, enterococcus, and Other Catalase-Negative Gram-Positive Cocci

Aerobic Gram-Positive Bacilli

Neisseria Species and Moraxella Catarrhalis

Haemophilus and Other Fastidious Gram-Negative Bacilli

Enterobacteriaceae

Vibrio,aeromonas,plesiomonas and Campylobacter Species

Nonfermenting and Miscellaneous Gram-Negative Bacilli

Anaerobes of Clinical Importance

Spirochetes

Chlamydia and Rickettsia

Mycoplasma and Ureaplasma

Mycobacterium Tuberculosis and Other Nontuberculosis Mycobacteria

Medically Significant Fungi

Diagnostic Parasitology

Clinical Virology

Agents of Bioterror

Biofilms: Architects of Disease

Upper and Lower Respiratory Tract Infections

Skin and Soft Tissue Infections

Gastrointestinal Infections and Food Poisoning

Infections of the Central Nervous System

Bacteremia and Sepsis

Urinary Tract Infections

Genital Infections and Sexually Transmitted Diseases

Infections in Special Populations

Zoonotic Diseases

Ocular Infections

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