Question 1

Sodium hydroxide, Zephiran-trisodium phosphate, N-acetyl-L-cysteine-NaOH, and oxalic acid are associated with

Accepted Answer

A) methods of cultivating mycobacterium in vitro. 
B) digestion and decontamination of specimens for mycobacterial studies. 
C) identification of clinically significant Mycobacterium. 
D) antimicrobial susceptibility testing of mycobacterial species. 
A) methods of cultivating mycobacterium in vitro. 
B) digestion and decontamination of specimens for mycobacterial studies. 
C) identification of clinically significant Mycobacterium. 
D) antimicrobial susceptibility testing of mycobacterial species.

Question 2

The type of specimen collected for mycobacterial studies that must be neutralized with 10% sodium carbonate is

Accepted Answer

A) spontaneously produced sputum. 
B) gastric lavage. 
C) transtracheal aspiration. 
D) bronchial lavage. 
A) spontaneously produced sputum. 
B) gastric lavage. 
C) transtracheal aspiration. 
D) bronchial lavage.

Question 3

The stain that requires heating the slide for better penetration of stain into the mycobacterial cell wall is known as the

Accepted Answer

A) Kinyoun. 
B) Ziehl-Neelsen. 
C) fluorochrome. 
D) carbolfuchsin. 
E) All of the above are correct. 
A) Kinyoun. 
B) Ziehl-Neelsen. 
C) fluorochrome. 
D) carbolfuchsin. 
E) All of the above are correct.

Question 4

Specimens for Mycobacterium studies that require concentration, decontamination, or liquefaction include

Accepted Answer

A) CSF and other body fluids. 
B) sputum. 
C) urine. 
D) swabs and wound aspirates. 
E) All of the above are correct. 
A) CSF and other body fluids. 
B) sputum. 
C) urine. 
D) swabs and wound aspirates. 
E) All of the above are correct.

Question 5

Of the following species of mycobacterium, which causes a chronic disease of the skin, mucous membranes, and nerve tissue and has not yet been cultivated in vitro?

Accepted Answer

A) M.paratuberculosis 
B) M.lepraemurium 
C) M.phlei 
D) M.leprae 
A) M.paratuberculosis 
B) M.lepraemurium 
C) M.phlei 
D) M.leprae

Question 6

Optimal conditions for most mycobacterial cultures are

Accepted Answer

A) 25° to 30° C in the dark in an atmosphere of 2% carbon dioxide and high humidity. 
B) 35° C in the dark in an atmosphere of 5% to 10% carbon dioxide and high humidity. 
C) 35° C in the dark in a microaerophilic atmosphere of 10% carbon dioxide and high humidity. 
D) 32° C in the dark in an atmosphere of decreased carbon dioxide and increased humidity. 
A) 25° to 30° C in the dark in an atmosphere of 2% carbon dioxide and high humidity. 
B) 35° C in the dark in an atmosphere of 5% to 10% carbon dioxide and high humidity. 
C) 35° C in the dark in a microaerophilic atmosphere of 10% carbon dioxide and high humidity. 
D) 32° C in the dark in an atmosphere of decreased carbon dioxide and increased humidity.

Question 7

Known to cause an inflammatory bowel disease referred to as Johne's disease in cattle, sheep, and goats, this organism has been isolated from the bowel mucosa of patients with Crohn's disease.

Accepted Answer

A) M.paratuberculosis 
B) M.avium-intracellulare 
C) M.phlei 
D) M.lepraemurium 
A) M.paratuberculosis 
B) M.avium-intracellulare 
C) M.phlei 
D) M.lepraemurium

Question 8

The nonpathogenic Mycobacterium commonly found in tap water, which has been a problem when tap water or deionized water has been used in the preparation of smears, is

Accepted Answer

A) M.paratuberculosis. 
B) M.lepraemurium. 
C) M.phlei. 
D) M.gordonae. 
A) M.paratuberculosis. 
B) M.lepraemurium. 
C) M.phlei. 
D) M.gordonae.

Exam 42: Mycobacteria

Sodium hydroxide, Zephiran-trisodium phosphate, N-acetyl-L-cysteine-NaOH, and oxalic acid are associated with

The type of specimen collected for mycobacterial studies that must be neutralized with 10% sodium carbonate is

The stain that requires heating the slide for better penetration of stain into the mycobacterial cell wall is known as the

Specimens for Mycobacterium studies that require concentration, decontamination, or liquefaction include

Of the following species of mycobacterium, which causes a chronic disease of the skin, mucous membranes, and nerve tissue and has not yet been cultivated in vitro?

Optimal conditions for most mycobacterial cultures are

Known to cause an inflammatory bowel disease referred to as Johne's disease in cattle, sheep, and goats, this organism has been isolated from the bowel mucosa of patients with Crohn's disease.

The nonpathogenic Mycobacterium commonly found in tap water, which has been a problem when tap water or deionized water has been used in the preparation of smears, is

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

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