Question 1

Growth of most Haemophilus species in culture is enhanced by

Accepted Answer

A) incubation at 33° to 35° C. 
B) high humidity. 
C) 5% to 10% carbon dioxide (CO₂). 
D) enriched special media. 
E) All of the above are correct. 
A) incubation at 33° to 35° C. 
B) high humidity. 
C) 5% to 10% carbon dioxide (CO₂). 
D) enriched special media. 
E) All of the above are correct. C

Question 2

With one exception, species of the genus Haemophilus require hemin and nicotine adenine dinucleotide (NAD) for in vitro growth.Which is the exception?

Accepted Answer

A) Haemophilus influenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.parainfluenzae. 
A) Haemophilus influenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.parainfluenzae. C

Question 3

Chancroid is the sexually transmitted disease caused by

Accepted Answer

A) Haemophilus influenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.parainfluenzae. 
A) Haemophilus influenzae. 
B) H.ducreyi. 
C) H.aphrophilus. 
D) H.parainfluenzae. B

Question 4

Growth of H.ducreyi in culture requires

Accepted Answer

A) incubation at 33° to 35° C. 
B) high humidity. 
C) increased CO₂. 
D) special media. 
E) All of the above are correct. 
A) incubation at 33° to 35° C. 
B) high humidity. 
C) increased CO₂. 
D) special media. 
E) All of the above are correct.

Question 5

In a phenomenon known as satelliting, tiny colonies of Haemophilus spp.may be seen growing on sheep blood agar very close to colonies of

Accepted Answer

A) Streptococcus pyogenes. 
B) Staphylococcus aureus. 
C) Micrococcus species. 
D) other Haemophilus species. 
A) Streptococcus pyogenes. 
B) Staphylococcus aureus. 
C) Micrococcus species. 
D) other Haemophilus species.

Question 6

Match the microscopic morphology with the most likely agent.
-Coccobacilli or small rods

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 7

Isolates from CSF that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, require factor V but not X, and are nonhemolytic on rabbit or horse blood may be identified as

Accepted Answer

A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae 
A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae

Question 8

The major difference between infections caused by type b H.influenzae and those infections caused by nontypeable strains is that

Accepted Answer

A) infections caused by type b H.influenzae are often systemic and life-threatening. 
B) infections caused by other Haemophilus spp.occur much more frequently. 
C) the number of type b H.influenzae infections has been greatly increased. 
D) All of the above are correct. 
E) None of the above are correct. 
A) infections caused by type b H.influenzae are often systemic and life-threatening. 
B) infections caused by other Haemophilus spp.occur much more frequently. 
C) the number of type b H.influenzae infections has been greatly increased. 
D) All of the above are correct. 
E) None of the above are correct.

Question 9

Match the microscopic morphology with the most likely agent.
-Small coccobacilli or short rods with occasional cells appearing as tangled filaments

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 10

Match the microscopic morphology with the most likely agent.
-Long, slender rods

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 11

Isolates from eye swabs that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as

Accepted Answer

A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae 
A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae

Question 12

Isolates from CSF or respiratory tract specimens that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as

Accepted Answer

A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae 
A) Haemophilus parainfluenzae. 
B) H.influenzae. 
C) H.aphrophilus. 
D) H.influenzae

Question 13

Match the microscopic morphology with the most likely agent.
-Pleomorphic rods

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 14

H.ducreyi requires what special media to grow?

Accepted Answer

A) Heart infusion-based agar supplemented with 10% IsoVitaleX and vancomycin 
B) Mueller-Hinton-based chocolate agar, supplemented with 1% IsoVitaleX and vancomycin 
C) Heart infusion-based chocolate agar, supplemented with 10% fetal bovine serum and vancomycin 
D) Mueller-Hinton-based agar, supplemented with 10% IsoVitaleX and vancomycin 
A) Heart infusion-based agar supplemented with 10% IsoVitaleX and vancomycin 
B) Mueller-Hinton-based chocolate agar, supplemented with 1% IsoVitaleX and vancomycin 
C) Heart infusion-based chocolate agar, supplemented with 10% fetal bovine serum and vancomycin 
D) Mueller-Hinton-based agar, supplemented with 10% IsoVitaleX and vancomycin

Question 15

Match the microscopic morphology with the most likely agent.
-Small pleomorphic rods or long filamentous forms

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 16

Of the following media, which provides the factors necessary for the growth of Haemophilus spp.?

Accepted Answer

A) 5% sheep blood agar 
B) Brain heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
E) All of the above are correct. 
A) 5% sheep blood agar 
B) Brain heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
E) All of the above are correct.

Question 17

In a phenomenon known as satelliting, tiny colonies of Haemophilus spp.may be seen growing on sheep blood agar very close to colonies of bacteria that can produce

Accepted Answer

A) hemin. 
B) nicotine adenine dinucleotide (NA
D) . 
C) vitamin K. 
D) cysteine. 
A) hemin. 
B) nicotine adenine dinucleotide (NA
D) . 
C) vitamin K. 
D) cysteine.

Question 18

Of the following media, which provides the NAD necessary for the growth of Haemophilus spp.?

Accepted Answer

A) 5% sheep blood agar 
B) Brain heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
E) All of the above are correct. 
A) 5% sheep blood agar 
B) Brain heart infusion agar 
C) Chocolate agar 
D) Nutrient agar 
E) All of the above are correct.

Question 19

Match the microscopic morphology with the most likely agent.
-Short to medium-length bacilli

Accepted Answer

A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae 
A) H. haemolyticus 
B) H. influenzae biotype aegypticus 
C) H. influenzae 
D) H. segnis 
E) H. parainfluenzae

Question 20

Actinobacillus actinomycetemcomitans can be distinguished from Haemophilus aphrophilus by

Accepted Answer

A) its negative test for catalase. 
B) its positive test for lactose fermentation. 
C) its positive test for catalase. 
D) its negative test for oxidase. 
E) All of the above are correct. 
A) its negative test for catalase. 
B) its positive test for lactose fermentation. 
C) its positive test for catalase. 
D) its negative test for oxidase. 
E) All of the above are correct.

Exam 31: Haemophilus

Growth of most Haemophilus species in culture is enhanced by

With one exception, species of the genus Haemophilus require hemin and nicotine adenine dinucleotide (NAD) for in vitro growth.Which is the exception?

Chancroid is the sexually transmitted disease caused by

Growth of H.ducreyi in culture requires

In a phenomenon known as satelliting, tiny colonies of Haemophilus spp.may be seen growing on sheep blood agar very close to colonies of

Match the microscopic morphology with the most likely agent. -Coccobacilli or small rods

Isolates from CSF that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, require factor V but not X, and are nonhemolytic on rabbit or horse blood may be identified as

The major difference between infections caused by type b H.influenzae and those infections caused by nontypeable strains is that

Match the microscopic morphology with the most likely agent. -Small coccobacilli or short rods with occasional cells appearing as tangled filaments

Match the microscopic morphology with the most likely agent. -Long, slender rods

Isolates from eye swabs that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as

Isolates from CSF or respiratory tract specimens that are gram-negative coccobacilli, grow on chocolate agar in CO₂ but not blood agar, and are porphyrin-negative and nonhemolytic on rabbit or horse blood may be identified as

Match the microscopic morphology with the most likely agent. -Pleomorphic rods

H.ducreyi requires what special media to grow?

Match the microscopic morphology with the most likely agent. -Small pleomorphic rods or long filamentous forms

Of the following media, which provides the factors necessary for the growth of Haemophilus spp.?

In a phenomenon known as satelliting, tiny colonies of Haemophilus spp.may be seen growing on sheep blood agar very close to colonies of bacteria that can produce

Of the following media, which provides the NAD necessary for the growth of Haemophilus spp.?

Match the microscopic morphology with the most likely agent. -Short to medium-length bacilli

Actinobacillus actinomycetemcomitans can be distinguished from Haemophilus aphrophilus by

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory