Question 1

Polyclonal antibodies are those that

Accepted Answer

A) react with many different types of antigens. 
B) react with many species of bacteria within the same genus. 
C) react with many different antigens of one specific organism. 
D) can be produced from many different species of host organisms. 
A) react with many different types of antigens. 
B) react with many species of bacteria within the same genus. 
C) react with many different antigens of one specific organism. 
D) can be produced from many different species of host organisms. C

Question 2

A modification of the Ouchterlony method that speeds up migration of an antigen and antibody by applying an electrical current is referred to as

Accepted Answer

A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay. 
A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay. B

Question 3

The immunochemical test that involves antigen present in a specimen binding to the combining sites of the antibody exposed on the surfaces of latex beads is referred to as

Accepted Answer

A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay. 
A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay. C

Question 4

A test procedure that applies a specific fluorescent dye-antibody complex to a slide to which the patient specimen has been applied is referred to as

Accepted Answer

A) a solid-phase enzyme immunoassay. 
B) a direct fluorescent antibody test. 
C) an indirect fluorescent antibody test. 
D) a radioimmunoassay. 
A) a solid-phase enzyme immunoassay. 
B) a direct fluorescent antibody test. 
C) an indirect fluorescent antibody test. 
D) a radioimmunoassay.

Question 5

The major advantage of immunofluorescent microscopy assays is that

Accepted Answer

A) extensive training is not necessary. 
B) it serves as an excellent way to preserve the reaction for referral and archiving. 
C) they allow visual assessment of the adequacy of a specimen. 
D) fluorescent microscopes are easily accessible to most laboratories. 
A) extensive training is not necessary. 
B) it serves as an excellent way to preserve the reaction for referral and archiving. 
C) they allow visual assessment of the adequacy of a specimen. 
D) fluorescent microscopes are easily accessible to most laboratories.

Question 6

Monoclonal antibodies are those that

Accepted Answer

A) react with many different types of antigens. 
B) react with many species of bacteria within the same genus. 
C) react with many different antigens of one specific organism. 
D) can be produced from many different species of host organisms. 
A) react with many different types of antigens. 
B) react with many species of bacteria within the same genus. 
C) react with many different antigens of one specific organism. 
D) can be produced from many different species of host organisms.

Question 7

The difference between latex agglutination and coagglutination is

Accepted Answer

A) the type of particle used to carry the antibody. 
B) the type of target organism involved. 
C) that coagglutination is less specific but more sensitive. 
D) coagglutination can be used for direct antigen detection but not latex agglutination. 
A) the type of particle used to carry the antibody. 
B) the type of target organism involved. 
C) that coagglutination is less specific but more sensitive. 
D) coagglutination can be used for direct antigen detection but not latex agglutination.

Question 8

The precipitin test that involves antigen and antibody diffusing toward each other, producing a visible precipitin band at the point at which they meet is referred to as

Accepted Answer

A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay. 
A) double immunodiffusion. 
B) counterimmunoelectrophoresis. 
C) particle agglutination. 
D) enzyme immunoassay.

Exam 9: Immunochemical Methods Used for Organism Detection

Polyclonal antibodies are those that

A modification of the Ouchterlony method that speeds up migration of an antigen and antibody by applying an electrical current is referred to as

The immunochemical test that involves antigen present in a specimen binding to the combining sites of the antibody exposed on the surfaces of latex beads is referred to as

A test procedure that applies a specific fluorescent dye-antibody complex to a slide to which the patient specimen has been applied is referred to as

The major advantage of immunofluorescent microscopy assays is that

Monoclonal antibodies are those that

The difference between latex agglutination and coagglutination is

The precipitin test that involves antigen and antibody diffusing toward each other, producing a visible precipitin band at the point at which they meet is referred to as

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

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