Question 1

Match the description to the most likely identification.
-Porphyromonas spp.

Accepted Answer

A) Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile 
B) bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative 
C) fluoresce brick red or brown to black pigment 
D) reduces nitrate and requires formate and fumarate for growth in broth culture 
E) sensitive to kanamycin and fluoresce a chartreuse color 
A) Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile 
B) bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative 
C) fluoresce brick red or brown to black pigment 
D) reduces nitrate and requires formate and fumarate for growth in broth culture 
E) sensitive to kanamycin and fluoresce a chartreuse color

Question 2

The most likely identification of an anaerobic gram-positive bacillus that produces spores is

Accepted Answer

A) Bifidobacterium. 
B) Eubacterium. 
C) Eggerthella. 
D) Clostridium. 
E) Mobiluncus. 
A) Bifidobacterium. 
B) Eubacterium. 
C) Eggerthella. 
D) Clostridium. 
E) Mobiluncus.

Question 3

The most likely identification of an anaerobic gram-negative coccus is

Accepted Answer

A) Porphyromonas spp. 
B) Veillonella. 
C) Leptotrichia. 
D) Fusobacterium spp. 
E) Bacteroides ureolyticus. 
A) Porphyromonas spp. 
B) Veillonella. 
C) Leptotrichia. 
D) Fusobacterium spp. 
E) Bacteroides ureolyticus.

Question 4

The most likely identification of an anaerobic gram-positive coccus that is resistant to metronidazole is

Accepted Answer

A) Streptococcus. 
B) Peptostreptococcus. 
C) Leptotrichia. 
D) Peptococcus. 
E) Finegoldia. 
A) Streptococcus. 
B) Peptostreptococcus. 
C) Leptotrichia. 
D) Peptococcus. 
E) Finegoldia.

Question 5

The most likely identification of a bile-sensitive, kanamycin-resistant, catalase-negative, and indole-negative anaerobic Gram negative bacilli is

Accepted Answer

A) nonpigmented Prevotella. 
B) Porphyromonas spp. 
C) Bacteroides fragilis group. 
D) pigmented Prevotella. 
E) Bacteroides ureolyticus. 
A) nonpigmented Prevotella. 
B) Porphyromonas spp. 
C) Bacteroides fragilis group. 
D) pigmented Prevotella. 
E) Bacteroides ureolyticus.

Question 6

Optimal recovery and identification of anaerobic bacteria in clinical specimens occurs when

Accepted Answer

A) the initial incubation period is at 35° to 37° C for 48 hours. 
B) plates are incubated for at least 3 days before discarding as negative. 
C) the initial incubation period is at 35° to 37° C for 24 hours. 
D) thioglycollate broth can be inspected daily for 3 days. 
A) the initial incubation period is at 35° to 37° C for 48 hours. 
B) plates are incubated for at least 3 days before discarding as negative. 
C) the initial incubation period is at 35° to 37° C for 24 hours. 
D) thioglycollate broth can be inspected daily for 3 days.

Question 7

In a clinical specimen, sulfur granules strongly indicate the presence of which anaerobe?

Accepted Answer

A) Actinomyces spp. 
B) Propionibacterium spp. 
C) Eubacterium nodatum 
D) All of the above are correct. 
A) Actinomyces spp. 
B) Propionibacterium spp. 
C) Eubacterium nodatum 
D) All of the above are correct.

Question 8

Complete identification of anaerobes is

Accepted Answer

A) relatively inexpensive for the average clinical laboratory. 
B) complicated and often requires several different testing methods. 
C) performed in most average clinical laboratories. 
D) necessary in most cases to ensure adequate treatment. 
E) All of the above are correct. 
A) relatively inexpensive for the average clinical laboratory. 
B) complicated and often requires several different testing methods. 
C) performed in most average clinical laboratories. 
D) necessary in most cases to ensure adequate treatment. 
E) All of the above are correct.

Question 9

The primary plating medium for inoculating anaerobic specimens includes

Accepted Answer

A) anaerobic blood agar. 
B) laked kanamycin-vancomycin blood agar. 
C) aerobic 5% sheep blood agar. 
D) thioglycollate. 
E) All of the above are correct. 
A) anaerobic blood agar. 
B) laked kanamycin-vancomycin blood agar. 
C) aerobic 5% sheep blood agar. 
D) thioglycollate. 
E) All of the above are correct.

Exam 41: Laboratory Considerations

Match the description to the most likely identification. -Porphyromonas spp.

The most likely identification of an anaerobic gram-positive bacillus that produces spores is

The most likely identification of an anaerobic gram-negative coccus is

The most likely identification of an anaerobic gram-positive coccus that is resistant to metronidazole is

The most likely identification of a bile-sensitive, kanamycin-resistant, catalase-negative, and indole-negative anaerobic Gram negative bacilli is

Optimal recovery and identification of anaerobic bacteria in clinical specimens occurs when

In a clinical specimen, sulfur granules strongly indicate the presence of which anaerobe?

Complete identification of anaerobes is

The primary plating medium for inoculating anaerobic specimens includes

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

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