Question 1

Aspiration of peritoneal fluid using a needle and syringe

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable A

Question 2

Percutaneous lung aspirate

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable A

Question 3

Feces in a sterile specimen cup

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable B

Question 4

Nasopharyngeal swab

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 5

Expectorated sputum

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 6

Rectal swab

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 7

Urine via catheter

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 8

During specimen collection, an important thing to remember when handling a collection device with an oxygen-free atmosphere is

Accepted Answer

A) to take care not to tip the container. 
B) to hold the collected specimen in the refrigerator until processing. 
C) to hold the collected specimen in the incubator until processing. 
D) to avoid opening the container in an aerobic atmosphere. 
A) to take care not to tip the container. 
B) to hold the collected specimen in the refrigerator until processing. 
C) to hold the collected specimen in the incubator until processing. 
D) to avoid opening the container in an aerobic atmosphere.

Question 9

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of

Accepted Answer

A) 20% to 30% nitrogen, 5% to 10% hydrogen, and 70% to 80% CO₂. 
B) 80% to 90% oxygen, 5% to 10% hydrogen, and 5% to 10% CO₂. 
C) 80% to 90% nitrogen, 5% to 10% hydrogen, and 5% to 10% CO₂. 
D) 80% to 90% nitrogen, 5% to 10% oxygen, and 5% to 10% CO₂. 
A) 20% to 30% nitrogen, 5% to 10% hydrogen, and 70% to 80% CO₂. 
B) 80% to 90% oxygen, 5% to 10% hydrogen, and 5% to 10% CO₂. 
C) 80% to 90% nitrogen, 5% to 10% hydrogen, and 5% to 10% CO₂. 
D) 80% to 90% nitrogen, 5% to 10% oxygen, and 5% to 10% CO₂.

Question 10

Recapping a syringe and transporting the needle and syringe to the laboratory ensures the preservation of the anaerobic nature of this type of specimen and

Accepted Answer

A) 	is the method of a. choice for transporting specimens for anaerobic culture. 
B) 	is not acceptable for transporting specimens for anaerobic culture because of safety concerns involving needlestick injuries. 
C) 	is rapidly replacing other methods of transporting specimens for anaerobic culture because of the effective preservation of the anaerobic nature of this type of specimen. 
D) 	is the method of choice for transporting fluid and other aspirated specimens for anaerobic culture. 
A) 	is the method of a. choice for transporting specimens for anaerobic culture. 
B) 	is not acceptable for transporting specimens for anaerobic culture because of safety concerns involving needlestick injuries. 
C) 	is rapidly replacing other methods of transporting specimens for anaerobic culture because of the effective preservation of the anaerobic nature of this type of specimen. 
D) 	is the method of choice for transporting fluid and other aspirated specimens for anaerobic culture.

Question 11

Tissue biopsy taken from a puncture wound

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 12

When activating a hydrogen and CO₂ generator system used for creating an anaerobic atmosphere, indications that the catalyst and generator envelope are functioning properly include

Accepted Answer

A) production of heat within a few minutes. 
B) development of moisture on the walls of the jar. 
C) a change in color of the methylene blue or resazurin indicator. 
D) All of the above are correct. 
A) production of heat within a few minutes. 
B) development of moisture on the walls of the jar. 
C) a change in color of the methylene blue or resazurin indicator. 
D) All of the above are correct.

Question 13

Urine via percutaneous aspiration

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 14

Cervical swab

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 15

Pre-reduced, anaerobically sterilized (PRAS) media

Accepted Answer

A) are produced, packaged, shipped, and stored under anaerobic conditions. 
B) have a short shelf life of only 2 weeks. 
C) enable the culture to be incubated in the regular incubator under aerobic conditions. 
D) All of the above are correct. 
A) are produced, packaged, shipped, and stored under anaerobic conditions. 
B) have a short shelf life of only 2 weeks. 
C) enable the culture to be incubated in the regular incubator under aerobic conditions. 
D) All of the above are correct.

Question 16

Bronchial washing collected with double lumen plugged catheter

Accepted Answer

A) Acceptable 
B) Not acceptable 
A) Acceptable 
B) Not acceptable

Question 17

Actinomyces spp., Bifidobacterium spp., and Clostridium spp.can be described as

Accepted Answer

A) aerotolerant organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under microaerobic conditions. 
B) strictly anaerobic organisms that cannot grow in the presence of either reduced or atmospheric oxygen but grow only under anaerobic conditions. 
C) microaerophilic organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under anaerobic conditions. 
D) facultatively anaerobic organisms that grow in the presence or absence of atmospheric oxygen. 
A) aerotolerant organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under microaerobic conditions. 
B) strictly anaerobic organisms that cannot grow in the presence of either reduced or atmospheric oxygen but grow only under anaerobic conditions. 
C) microaerophilic organisms that can grow in the presence of either reduced or atmospheric oxygen but grow best under anaerobic conditions. 
D) facultatively anaerobic organisms that grow in the presence or absence of atmospheric oxygen.

Exam 40: Overview and General Considerations

Aspiration of peritoneal fluid using a needle and syringe

Percutaneous lung aspirate

Feces in a sterile specimen cup

Nasopharyngeal swab

Expectorated sputum

Rectal swab

Urine via catheter

During specimen collection, an important thing to remember when handling a collection device with an oxygen-free atmosphere is

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of

Recapping a syringe and transporting the needle and syringe to the laboratory ensures the preservation of the anaerobic nature of this type of specimen and

Tissue biopsy taken from a puncture wound

When activating a hydrogen and CO₂ generator system used for creating an anaerobic atmosphere, indications that the catalyst and generator envelope are functioning properly include

Urine via percutaneous aspiration

Cervical swab

Pre-reduced, anaerobically sterilized (PRAS) media

Bronchial washing collected with double lumen plugged catheter

Actinomyces spp., Bifidobacterium spp., and Clostridium spp.can be described as

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

Filters