Question 1

A convenient and reliable identification scheme for P.aeruginosa involves the following conventional tests:

Accepted Answer

A) Oxidase, TSI slant, growth at 42° C on Mueller-Hinton agar or trypticase soy agar 
B) Catalase, LIA slant, growth at 42° C on Mueller-Hinton agar or trypticase soy agar 
C) Oxidase, TSI slant, growth at 24° C on Mueller-Hinton agar or trypticase soy agar 
D) Catalase, TSI slant, growth at 42° C on sheep blood agar or blood trypticase soy agar 
A) Oxidase, TSI slant, growth at 42° C on Mueller-Hinton agar or trypticase soy agar 
B) Catalase, LIA slant, growth at 42° C on Mueller-Hinton agar or trypticase soy agar 
C) Oxidase, TSI slant, growth at 24° C on Mueller-Hinton agar or trypticase soy agar 
D) Catalase, TSI slant, growth at 42° C on sheep blood agar or blood trypticase soy agar A

Question 2

The disease referred to as melioidosis is caused by

Accepted Answer

A) Pseudomonas aeruginosa. 
B) Ralstonia pickettii. 
C) Burkholderia mallei. 
D) Burkholderia pseudomallei. 
A) Pseudomonas aeruginosa. 
B) Ralstonia pickettii. 
C) Burkholderia mallei. 
D) Burkholderia pseudomallei. D

Question 3

Production of exotoxin A, which kills host cells by inhibiting protein synthesis, and production of several proteolytic enzymes and hemolysins that destroy cells and tissue are factors that contribute to pathogenicity of

Accepted Answer

A) Pseudomonas aeruginosa. 
B) Burkholderia cepacia. 
C) Ralstonia pickettii. 
D) Burkholderia mallei. 
A) Pseudomonas aeruginosa. 
B) Burkholderia cepacia. 
C) Ralstonia pickettii. 
D) Burkholderia mallei. A

Question 4

Excluding Enterobacteriaceae, name the second most common gram-negative bacilli encountered in clinical specimens.

Accepted Answer

A) Pseudomonas 
B) Burkholderia 
C) Ralstonia 
D) Vibrio 
A) Pseudomonas 
B) Burkholderia 
C) Ralstonia 
D) Vibrio

Question 5

Which best describes the source of Burkholderia cepacia infections?

Accepted Answer

A) Plants, soil, and water 
B) Medical devices medical solutions 
C) Contaminated foods 
D) None of the above are correct. 
E) All of the above are correct. 
A) Plants, soil, and water 
B) Medical devices medical solutions 
C) Contaminated foods 
D) None of the above are correct. 
E) All of the above are correct.

Question 6

An organism described as oxidase-positive, TSI slant with an alkaline/no change, good growth at 42° C, and producing bright bluish green pigment on Mueller-Hinton agar is most likely

Accepted Answer

A) Pseudomonas aeruginosa. 
B) Ralstonia pickettii. 
C) Burkholderia mallei. 
D) Burkholderia pseudomallei. 
A) Pseudomonas aeruginosa. 
B) Ralstonia pickettii. 
C) Burkholderia mallei. 
D) Burkholderia pseudomallei.

Question 7

Of the species of Burkholderia, which is the one most commonly found in clinical specimens?

Accepted Answer

A) Burkholderia ralstonia 
B) Burkholderia pseudomallei 
C) Burkholderia mallei 
D) Burkholderia cepacia 
A) Burkholderia ralstonia 
B) Burkholderia pseudomallei 
C) Burkholderia mallei 
D) Burkholderia cepacia

Question 8

All Brevundimonas, Burkholderia, Ralstonia, Pseudomonas, and Acidovorax share which of the following characteristics?

Accepted Answer

A) Oxidase-positive 
B) Utilize glucose oxidatively 
C) Able to grow on MacConkey agar 
D) None of the above are correct. 
E) All of the above are correct. 
A) Oxidase-positive 
B) Utilize glucose oxidatively 
C) Able to grow on MacConkey agar 
D) None of the above are correct. 
E) All of the above are correct.

Exam 21: Pseudomonas, Burkholderia, and Similar Organisms

A convenient and reliable identification scheme for P.aeruginosa involves the following conventional tests:

The disease referred to as melioidosis is caused by

Production of exotoxin A, which kills host cells by inhibiting protein synthesis, and production of several proteolytic enzymes and hemolysins that destroy cells and tissue are factors that contribute to pathogenicity of

Excluding Enterobacteriaceae, name the second most common gram-negative bacilli encountered in clinical specimens.

Which best describes the source of Burkholderia cepacia infections?

An organism described as oxidase-positive, TSI slant with an alkaline/no change, good growth at 42° C, and producing bright bluish green pigment on Mueller-Hinton agar is most likely

Of the species of Burkholderia, which is the one most commonly found in clinical specimens?

All Brevundimonas, Burkholderia, Ralstonia, Pseudomonas, and Acidovorax share which of the following characteristics?

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

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