Question 1

To read the hemolytic reaction on a blood agar plate accurately, the technologist must

Accepted Answer

A) hold the plate up to the light and observe the plate with the light coming from behind. 
B) hold the plate under the light and observe the plate with the light coming from the top. 
C) hold the plate over a white background. 
D) examine the plate using reflected and transmitted light. 
A) hold the plate up to the light and observe the plate with the light coming from behind. 
B) hold the plate under the light and observe the plate with the light coming from the top. 
C) hold the plate over a white background. 
D) examine the plate using reflected and transmitted light.

Question 2

Aerobes grow best in

Accepted Answer

A) ambient air, which contains 21% oxygen (O₂) and a small amount (0.03%) of carbon dioxide. 
B) increased concentrations of CO₂ (5% to 10%) and approximately 15% O₂. 
C) 5% to 10% hydrogen (H₂), 5% to 10% CO₂, 80% to 90% nitrogen (N₂), and 0% O₂. 
D) reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%). 
A) ambient air, which contains 21% oxygen (O₂) and a small amount (0.03%) of carbon dioxide. 
B) increased concentrations of CO₂ (5% to 10%) and approximately 15% O₂. 
C) 5% to 10% hydrogen (H₂), 5% to 10% CO₂, 80% to 90% nitrogen (N₂), and 0% O₂. 
D) reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%).

Question 3

Which medium can be described as suppressing the growth of most gram-negative organisms while allowing gram-positive bacteria to grow?

Accepted Answer

A) Chocolate 
B) CNA (colistin, nalidixic acid) blood 
C) BHI (brain heart infusion) 
D) Thioglycollate 
A) Chocolate 
B) CNA (colistin, nalidixic acid) blood 
C) BHI (brain heart infusion) 
D) Thioglycollate

Question 4

Capnophiles grow best in

Accepted Answer

A) ambient air, which contains 21% oxygen (O₂) and a small amount (0.03%) of carbon dioxide. 
B) increased concentrations of CO₂ (5% to 10%) and approximately 15% O₂. 
C) 5% to 10% hydrogen (H₂), 5% to 10% CO₂, 80% to 90% nitrogen (N₂), and 0% O₂. 
D) reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%). 
A) ambient air, which contains 21% oxygen (O₂) and a small amount (0.03%) of carbon dioxide. 
B) increased concentrations of CO₂ (5% to 10%) and approximately 15% O₂. 
C) 5% to 10% hydrogen (H₂), 5% to 10% CO₂, 80% to 90% nitrogen (N₂), and 0% O₂. 
D) reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%).

Question 5

Which medium can be described as containing crystal violet dye to inhibit the growth of gram-positive bacteria and fungi, and allows many types of gram-negative bacilli to grow?

Accepted Answer

A) Thayer-Martin 
B) MacConkey 
C) PEA (phenylethyl alcohol) 
D) Hektoen 
A) Thayer-Martin 
B) MacConkey 
C) PEA (phenylethyl alcohol) 
D) Hektoen

Question 6

Which category of media can be described as containing some factor (or factors) that allows colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate?

Accepted Answer

A) Enrichment 
B) Supportive 
C) Selective 
D) Differential 
A) Enrichment 
B) Supportive 
C) Selective 
D) Differential

Question 7

What name is given to the type of hemolysis that produces incomplete hemolysis and a greening discoloration of the medium?

Accepted Answer

A) Beta hemolysis 
B) Gamma hemolysis 
C) Alpha hemolysis 
D) Kappa hemolysis 
A) Beta hemolysis 
B) Gamma hemolysis 
C) Alpha hemolysis 
D) Kappa hemolysis

Question 8

Which type of media is designed to distinguish microorganisms on the basis of certain growth characteristics?

Accepted Answer

A) Selective 
B) Nutritive 
C) Differential 
D) Definitive 
A) Selective 
B) Nutritive 
C) Differential 
D) Definitive

Question 9

Which category of media can be described as containing one or more agents that are inhibitory to all organisms except those being sought?

Accepted Answer

A) Enrichment 
B) Supportive 
C) Selective 
D) Differential 
A) Enrichment 
B) Supportive 
C) Selective 
D) Differential

Question 10

The role of the agar ingredient in culture medium is to

Accepted Answer

A) provide a nutritional base for bacterial metabolism. 
B) solidify the medium and provide a stable culture surface. 
C) sterilize the medium and prevent contamination. 
D) All of the above are correct. 
A) provide a nutritional base for bacterial metabolism. 
B) solidify the medium and provide a stable culture surface. 
C) sterilize the medium and prevent contamination. 
D) All of the above are correct.

Question 11

What name is given to the type of hemolysis that produces complete hemolysis and a clearing of the medium?

Accepted Answer

A) Beta hemolysis 
B) Gamma hemolysis 
C) Alpha hemolysis 
D) Kappa hemolysis 
A) Beta hemolysis 
B) Gamma hemolysis 
C) Alpha hemolysis 
D) Kappa hemolysis

Question 12

Cultivation of most medically important bacteria sought in the medical laboratory is done using incubators with temperatures maintained in what range?

Accepted Answer

A) 35° to 37° C 
B) 30° to 35° C 
C) 37° to 39° C 
D) 25° to 35° C 
A) 35° to 37° C 
B) 30° to 35° C 
C) 37° to 39° C 
D) 25° to 35° C

Question 13

Reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%) is the environmental condition that best suits which type of organism?

Accepted Answer

A) Aerobes 
B) Anaerobes 
C) Capnophiles 
D) Microaerophiles 
A) Aerobes 
B) Anaerobes 
C) Capnophiles 
D) Microaerophiles

Question 14

Which category of media would Löwenstein-Jensen best fit?

Accepted Answer

A) Selective 
B) Differential 
C) Selective and differential 
D) Supportive only 
A) Selective 
B) Differential 
C) Selective and differential 
D) Supportive only

Exam 7: Traditional Cultivation and Identification

To read the hemolytic reaction on a blood agar plate accurately, the technologist must

Aerobes grow best in

Which medium can be described as suppressing the growth of most gram-negative organisms while allowing gram-positive bacteria to grow?

Capnophiles grow best in

Which medium can be described as containing crystal violet dye to inhibit the growth of gram-positive bacteria and fungi, and allows many types of gram-negative bacilli to grow?

Which category of media can be described as containing some factor (or factors) that allows colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate?

What name is given to the type of hemolysis that produces incomplete hemolysis and a greening discoloration of the medium?

Which type of media is designed to distinguish microorganisms on the basis of certain growth characteristics?

Which category of media can be described as containing one or more agents that are inhibitory to all organisms except those being sought?

The role of the agar ingredient in culture medium is to

What name is given to the type of hemolysis that produces complete hemolysis and a clearing of the medium?

Cultivation of most medically important bacteria sought in the medical laboratory is done using incubators with temperatures maintained in what range?

Reduced O₂ (5% to 10%) and increased CO₂ (8% to 10%) is the environmental condition that best suits which type of organism?

Which category of media would Löwenstein-Jensen best fit?

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