Question 1

Biosafety Level 2 agents

Accepted Answer

A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include Mycobacterium tuberculosis, M.gordonae, and Brucella. 
D) require the use of maximum containment facilities. 
A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include Mycobacterium tuberculosis, M.gordonae, and Brucella. 
D) require the use of maximum containment facilities.

Question 2

To be sterilized in an autoclave, media or instruments should be

Accepted Answer

A) packed tightly to avoid dangerous pressure pockets. 
B) processed for 15 to 20 minutes at 121° centigrade and 15 psi pressure. 
C) processed for 30 to 60 minutes at 132° centigrade and 15 psi pressure. 
D) processed for at least 1 hour at 121° centigrade and 15 psi pressure. 
A) packed tightly to avoid dangerous pressure pockets. 
B) processed for 15 to 20 minutes at 121° centigrade and 15 psi pressure. 
C) processed for 30 to 60 minutes at 132° centigrade and 15 psi pressure. 
D) processed for at least 1 hour at 121° centigrade and 15 psi pressure.

Question 3

Certain procedures require the use of a biological safety cabinet.These include

Accepted Answer

A) processing specimens suspected of containing mycobacteria. 
B) manipulation of all moldlike fungi. 
C) all virology procedures. 
D) manipulation of respiratory secretions. 
E) all of the above. 
A) processing specimens suspected of containing mycobacteria. 
B) manipulation of all moldlike fungi. 
C) all virology procedures. 
D) manipulation of respiratory secretions. 
E) all of the above.

Question 4

Universal precautions in the microbiology laboratory apply to

Accepted Answer

A) all clinical specimens. 
B) clinical specimens that contain blood or body fluids. 
C) blood, body fluids, feces, sputum, saliva, urine, and vomitus. 
D) only specimens that are visibly bloody. 
A) all clinical specimens. 
B) clinical specimens that contain blood or body fluids. 
C) blood, body fluids, feces, sputum, saliva, urine, and vomitus. 
D) only specimens that are visibly bloody.

Question 5

Biosafety Level 1 agents

Accepted Answer

A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include Mycobacterium tuberculosis, M.gordonae, and Brucella. 
D) require the use of maximum containment facilities. 
A) include those that are the common agents of infectious disease. 
B) require only standard good laboratory technique. 
C) include Mycobacterium tuberculosis, M.gordonae, and Brucella. 
D) require the use of maximum containment facilities.

Question 6

The agency that published guidelines regarding the packaging of biohazardous specimens is known as

Accepted Answer

A) DHEC. 
B) IATA. 
C) CDC. 
D) EPA. 
A) DHEC. 
B) IATA. 
C) CDC. 
D) EPA.

Question 7

Class II biological safety cabinets

Accepted Answer

A) sterilize the air within the cabinet as it passes over the material in the cabinet. 
B) protect the environment from contamination by biologic agents by filtering the air as it is exhausted from the cabinet. 
C) direct the flow of contaminated air into the filters. 
D) are often called laminar flow hoods. 
E) All of the above are correct. 
A) sterilize the air within the cabinet as it passes over the material in the cabinet. 
B) protect the environment from contamination by biologic agents by filtering the air as it is exhausted from the cabinet. 
C) direct the flow of contaminated air into the filters. 
D) are often called laminar flow hoods. 
E) All of the above are correct.

Exam 4: Laboratory Safety

Biosafety Level 2 agents

To be sterilized in an autoclave, media or instruments should be

Certain procedures require the use of a biological safety cabinet.These include

Universal precautions in the microbiology laboratory apply to

Biosafety Level 1 agents

The agency that published guidelines regarding the packaging of biohazardous specimens is known as

Class II biological safety cabinets

Microbial Taxonomy

Bacterial Genetics Metabolism and Structure

Host-Microorganism Interactions

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria, Corynebacterium, and Similar Organisms

Erysipelothirix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Nonculturable Bacterial Agents

Cell Wall-Deficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections

Laboratory Methods in Basic Mycology

Laboratory Methods in Basic Virology

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes, Ears, and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections

Skin, Soft Tissue, and Wound Infections

Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Laboratory Physical Design, Management, and Organization

Quality in the Clinical Microbiology Laboratory

Infection Control

Sentinel Laboratory Response to Bioterrorism New

Filters