Exam 6: Molecular Biology of Bacteria

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The precursor of each new nucleotide in a strand of DNA is a

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One high- energy phosphate bond is required for the activation of an aminoacyl- tRNA.

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Proteins known as chaperones are found only in Bacteria, and their sequences vary from organism to organism.

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Discuss the initiation of DNA synthesis in Bacteria using the terms origin of replication, replication fork, and theta structures.

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During DNA synthesis, the RNA primer is removed by a(n)

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Sometimes it appears that bases in tRNA loops are actually paired with bases in other tRNA loops, which is not the case because pairing occurs exclusively within a given loop.

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Explain how Escherichia coli can grow with a doubling time of 20 minutes when chromosome replication takes 40 minutes.

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When several ribosomes are simultaneously translating a single mRNA molecule, the complex is called a(n)________ .

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A triplet of bases on an mRNA molecule is known as a(n) ________.

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Speculate on why the half- life of mRNA is short, while the half- lives of rRNA and tRNA are long.

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Explain the difference between transcription and translation.

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If a transcription unit contains two or more genes, these genes are then________, giving a single RNA molecule.

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Which statement is generally TRUE regarding protein synthesis?

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Ciprofloxacin, nalidixic acid, and novobiocin are effective antibiotics because they inhibit the activity of

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In Bacteria and most Archaea, the enzyme that introduces negative supercoils into DNA is known as

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Explain the concept of semiconservative replication.

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DNA replication always proceeds from the _________ of the incoming nucleotide to the _________ of the previously added nucleotide.

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Define genome in terms of the various DNA components of the cell or virus.

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An example of nucleotide pairing is

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In nature, the predominant form of DNA is supercoiled in a negative direction.

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