Question 1

The preparation of knockout mutants involves:

Accepted Answer

A) inserting a piece of DNA into the gene of interest. 
B) the use of closely packed DNA fragments attached to a glass slide. 
C) searching for homology. 
D) determining the number of base pairs in a gene. 
E) determining the rate of recombination. 
A) inserting a piece of DNA into the gene of interest. 
B) the use of closely packed DNA fragments attached to a glass slide. 
C) searching for homology. 
D) determining the number of base pairs in a gene. 
E) determining the rate of recombination.

Question 2

By definition, a transformed cell:

Accepted Answer

A) contains a plasmid. 
B) contains a recombinant DNA molecule. 
C) contains a virus. 
D) functions as a vector. 
E) divides in such a way so as to amplify the DNA fragment. 
A) contains a plasmid. 
B) contains a recombinant DNA molecule. 
C) contains a virus. 
D) functions as a vector. 
E) divides in such a way so as to amplify the DNA fragment.

Question 3

The ampR gene is used in recombinant DNA technology to:

Accepted Answer

A) produce β-galactosidase. 
B) clone a particular gene fragment. 
C) screen for the presence of cells containing recombinant plasmids. 
D) help the bacterium to survive on a medium containing lactose. 
E) transform a bacterial cell. 
A) produce β-galactosidase. 
B) clone a particular gene fragment. 
C) screen for the presence of cells containing recombinant plasmids. 
D) help the bacterium to survive on a medium containing lactose. 
E) transform a bacterial cell.

Question 4

Sticky-ended fragments can be joined by specific restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 5

"Golden rice" is notable because of its:

Accepted Answer

A) high β-carotene content. 
B) high starch content. 
C) resistance to herbicides. 
D) delayed senescence. 
E) resistance to insect larvae. 
A) high β-carotene content. 
B) high starch content. 
C) resistance to herbicides. 
D) delayed senescence. 
E) resistance to insect larvae.

Question 6

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

Accepted Answer

A) True 
 B)False

Question 7

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

Accepted Answer

A) True 
 B)False

Question 8

It was not until the discovery of ________ that organ and tissue culture became feasible.

Accepted Answer

A) hydroponics 
B) plant hormones 
C) the importance of sucrose in nutrient solutions 
D) the essential mineral elements 
E) biotechnology 
A) hydroponics 
B) plant hormones 
C) the importance of sucrose in nutrient solutions 
D) the essential mineral elements 
E) biotechnology

Question 9

Which of the following statements concerning restriction enzymes is FALSE?

Accepted Answer

A) They are a type of endonuclease. 
B) They recognize DNA sequences typically four to six nucleotides long. 
C) They recognize single-stranded DNA sequences. 
D) Some make straight cuts in the DNA. 
E) They recognize specific palindromic sequences. 
A) They are a type of endonuclease. 
B) They recognize DNA sequences typically four to six nucleotides long. 
C) They recognize single-stranded DNA sequences. 
D) Some make straight cuts in the DNA. 
E) They recognize specific palindromic sequences.

Question 10

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Accepted Answer

A) increasing starch content. 
B) increasing levels of β-carotene. 
C) stimulating fruit ripening. 
D) conferring resistance to pesticides. 
E) delaying flower wilting. 
A) increasing starch content. 
B) increasing levels of β-carotene. 
C) stimulating fruit ripening. 
D) conferring resistance to pesticides. 
E) delaying flower wilting.

Question 11

Which of the following statements about eukaryotic genomes is FALSE?

Accepted Answer

A) They are larger than those of prokaryotes. 
B) In general, multicellular eukaryotes have more DNA than unicellular eukaryotes. 
C) The greater the complexity of the organism, the more protein-coding genes it has. 
D) The roundworm Caenorhacditis has more genes than the fruit fly Drosophila. 
E) The plant Arabidopsis has more genes than the fruit fly Drosophila. 
A) They are larger than those of prokaryotes. 
B) In general, multicellular eukaryotes have more DNA than unicellular eukaryotes. 
C) The greater the complexity of the organism, the more protein-coding genes it has. 
D) The roundworm Caenorhacditis has more genes than the fruit fly Drosophila. 
E) The plant Arabidopsis has more genes than the fruit fly Drosophila.

Question 12

An example of a selectable marker gene is:

Accepted Answer

A) the gene for a green fluorescent protein. 
B) the luciferase gene. 
C) EcoRI. 
D) lacZ. 
E) ampR. 
A) the gene for a green fluorescent protein. 
B) the luciferase gene. 
C) EcoRI. 
D) lacZ. 
E) ampR.

Question 13

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

Accepted Answer

A) True 
 B)False

Question 14

A sticky-ended fragment can join with another DNA segment only if that segment:

Accepted Answer

A) has been cut with the same restriction enzyme. 
B) has been cut with the same ligase. 
C) is palindromic. 
D) is part of a plasmid. 
E) is part of a virus. 
A) has been cut with the same restriction enzyme. 
B) has been cut with the same ligase. 
C) is palindromic. 
D) is part of a plasmid. 
E) is part of a virus.

Question 15

The first eukaryotic genome to be sequenced was that of:

Accepted Answer

A) Haemophilus influenzae. 
B) Saccharomyces cerevisae. 
C) Arabidopsis thaliana. 
D) Agrobacterium tumefaciens. 
E) Caenorhabditis elegans. 
A) Haemophilus influenzae. 
B) Saccharomyces cerevisae. 
C) Arabidopsis thaliana. 
D) Agrobacterium tumefaciens. 
E) Caenorhabditis elegans.

Question 16

A search for homology would be used to determine the possible function of a gene.

Accepted Answer

A) True 
 B)False

Question 17

By definition, hydroponics is the technique for growing:

Accepted Answer

A) shoot tips. 
B) isolated leaf pieces. 
C) isolated roots 
D) plant embryos. 
E) plants without soil. 
A) shoot tips. 
B) isolated leaf pieces. 
C) isolated roots 
D) plant embryos. 
E) plants without soil.

Question 18

In Agrobacterium, genes that code for enzymes involved in the synthesis of the hormone auxin are found in the _____ region of the plasmid.

Accepted Answer

A) R 
B) O 
C) onc 
D) vir 
E) A 
A) R 
B) O 
C) onc 
D) vir 
E) A

Question 19

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Accepted Answer

A) True 
 B)False

Question 20

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Accepted Answer

A) True 
 B)False

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

The preparation of knockout mutants involves:

By definition, a transformed cell:

The ampR gene is used in recombinant DNA technology to:

Sticky-ended fragments can be joined by specific restriction enzymes.

"Golden rice" is notable because of its:

Agrobacterium tumefaciens receives the Ti plasmid from its host plant.

The Ti plasmid of Agrobacterium tumefaciens contains genes for plant hormones as well as genes for molecules that nourish the bacterium.

It was not until the discovery of ________ that organ and tissue culture became feasible.

Which of the following statements concerning restriction enzymes is FALSE?

Mutant forms of the ethylene receptor gene from Arabidopsis are inserted into plants for the purpose of:

Which of the following statements about eukaryotic genomes is FALSE?

An example of a selectable marker gene is:

DNA fragments having sticky ends can join with other fragments obtained by using the same restriction enzyme.

A sticky-ended fragment can join with another DNA segment only if that segment:

The first eukaryotic genome to be sequenced was that of:

A search for homology would be used to determine the possible function of a gene.

By definition, hydroponics is the technique for growing:

In Agrobacterium, genes that code for enzymes involved in the synthesis of the hormone auxin are found in the _____ region of the plasmid.

Particle bombardment and electroporation are the two techniques most widely used to transfer DNA into plants.

Identical DNA fragments are obtained by cutting the same DNA molecule with different restriction enzymes.

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

The amp^R gene is used in recombinant DNA technology to: