Question 1

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

Accepted Answer

A) R 
B) O 
C) onc 
D) vir 
E) A 
A) R 
B) O 
C) onc 
D) vir 
E) A B

Question 2

When the lacZ gene technique is used to detect recombinant DNA in host bacterial cells, the colonies will appear _______ if the cells have been successfully transformed.

Accepted Answer

A) red 
B) white 
C) blue 
D) green 
E) fluorescent 
A) red 
B) white 
C) blue 
D) green 
E) fluorescent B

Question 3

The Taq polymerase is used in recombinant DNA technology to:

Accepted Answer

A) locate specific DNA segments. 
B) determine the nucleotide sequence of a specific gene. 
C) make up to a millionfold copies of a DNA segment in a few hours. 
D) make cDNA. 
E) carry out "shotgun cloning." 
A) locate specific DNA segments. 
B) determine the nucleotide sequence of a specific gene. 
C) make up to a millionfold copies of a DNA segment in a few hours. 
D) make cDNA. 
E) carry out "shotgun cloning." C

Question 4

Which of the following is NOT one of the goals of functional genomics?

Accepted Answer

A) identifying the transcriptome 
B) identifying the proteome 
C) determining the function of genes 
D) determining which genes are expressed 
E) determining the location of genes on a chromosome 
A) identifying the transcriptome 
B) identifying the proteome 
C) determining the function of genes 
D) determining which genes are expressed 
E) determining the location of genes on a chromosome

Question 5

Micropropagation is used to obtain clones of plants.

Accepted Answer

A) True 
 B)False

Question 6

A knockout mutant has a particular gene inactivated.

Accepted Answer

A) True 
 B)False

Question 7

One advantage of meristem culture is that virus-free plants can be obtained.

Accepted Answer

A) True 
 B)False

Question 8

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

Accepted Answer

A) to delay leaf senescence 
B) to confer resistance to herbicides 
C) to confer resistance to BT toxin 
D) to increase starch content 
E) to increase glyphosate production 
A) to delay leaf senescence 
B) to confer resistance to herbicides 
C) to confer resistance to BT toxin 
D) to increase starch content 
E) to increase glyphosate production

Question 9

The transcriptome refers to all of the:

Accepted Answer

A) proteins encoded by the genome. 
B) glycoproteins transcribed by the genome. 
C) nucleotides present in a cell's DNA. 
D) nucleotides present in a cell's RNA. 
E) RNA molecules transcribed by the genome. 
A) proteins encoded by the genome. 
B) glycoproteins transcribed by the genome. 
C) nucleotides present in a cell's DNA. 
D) nucleotides present in a cell's RNA. 
E) RNA molecules transcribed by the genome.

Question 10

In recombinant DNA technology, reverse transcriptase is used to:

Accepted Answer

A) synthesize cDNA. 
B) synthesize mRNA. 
C) cut DNA into fragments. 
D) amplify a specific gene. 
E) transfer a plasmid into a cell. 
A) synthesize cDNA. 
B) synthesize mRNA. 
C) cut DNA into fragments. 
D) amplify a specific gene. 
E) transfer a plasmid into a cell.

Question 11

Suppose a plasmid containing a gene of interest plus the ampR gene is used to transform E.coli cells.When these cells are placed on a medium containing ampicillin, what will happen?

Accepted Answer

A) They will die. 
B) They will survive and grow. 
C) They will form blue colonies. 
D) They will glow with a green color. 
E) They will glow when provided with luciferin plus ATP. 
A) They will die. 
B) They will survive and grow. 
C) They will form blue colonies. 
D) They will glow with a green color. 
E) They will glow when provided with luciferin plus ATP.

Question 12

DNA ligase would most likely be used in recombinant DNA technology to:

Accepted Answer

A) function as a vector. 
B) screen for recombinant plasmids. 
C) stimulate a transformed cell to divide. 
D) cut DNA into fragments with sticky ends. 
E) join DNA fragments together. 
A) function as a vector. 
B) screen for recombinant plasmids. 
C) stimulate a transformed cell to divide. 
D) cut DNA into fragments with sticky ends. 
E) join DNA fragments together.

Question 13

Because meristems lack _________ tissues, meristem culture results in plants that lack _________.

Accepted Answer

A) vascular; viruses 
B) vascular; fungi 
C) epidermal; viruses 
D) epidermal; fungi 
E) epidermal; insect eggs 
A) vascular; viruses 
B) vascular; fungi 
C) epidermal; viruses 
D) epidermal; fungi 
E) epidermal; insect eggs

Question 14

Traditional breeding programs:

Accepted Answer

A) involve inserting a plasmid into a host cell. 
B) involve infecting a host plant with a virus. 
C) allow gene transfers between species incapable of hybridizing. 
D) depend on inherent genetic variability. 
E) allow the precise insertion of genes into a plant. 
A) involve inserting a plasmid into a host cell. 
B) involve infecting a host plant with a virus. 
C) allow gene transfers between species incapable of hybridizing. 
D) depend on inherent genetic variability. 
E) allow the precise insertion of genes into a plant.

Question 15

Which of the following statements about particle bombardment is FALSE?

Accepted Answer

A) It is used to deliver DNA but not RNA into a cell. 
B) It involves the use of gold or tungsten beads. 
C) It involves high-velocity microprojectiles. 
D) It involves the use of a gene gun. 
E) It has been used to create a virus-resistant strain of papaya. 
A) It is used to deliver DNA but not RNA into a cell. 
B) It involves the use of gold or tungsten beads. 
C) It involves high-velocity microprojectiles. 
D) It involves the use of a gene gun. 
E) It has been used to create a virus-resistant strain of papaya.

Question 16

The polymerase chain reaction is used to:

Accepted Answer

A) determine where gene promoters are active. 
B) produce millions of copies of a DNA segment in a short time. 
C) determine if a gene inserted into a cell is active or inactive. 
D) produce a cDNA library. 
E) transform a cell. 
A) determine where gene promoters are active. 
B) produce millions of copies of a DNA segment in a short time. 
C) determine if a gene inserted into a cell is active or inactive. 
D) produce a cDNA library. 
E) transform a cell.

Question 17

Agrobacterium tumefaciens is used to obtain transgenetic plants.

Accepted Answer

A) True 
 B)False

Question 18

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

Accepted Answer

A) True 
 B)False

Question 19

Glyphosate is:

Accepted Answer

A) an enzyme in amino acid synthesis. 
B) a mutant gene from Arabidopsis. 
C) toxic to animals. 
D) an herbicide that kills all plants 
E) a mutant gene from Salmonella. 
A) an enzyme in amino acid synthesis. 
B) a mutant gene from Arabidopsis. 
C) toxic to animals. 
D) an herbicide that kills all plants 
E) a mutant gene from Salmonella.

Question 20

A genetic map is different from a physical map in that a genetic map is:

Accepted Answer

A) used to determine the function of genes. 
B) based on rates of recombination. 
C) used to determine which genes are inactivate. 
D) based on direct DNA sequencing. 
E) based on evolutionary relationships. 
A) used to determine the function of genes. 
B) based on rates of recombination. 
C) used to determine which genes are inactivate. 
D) based on direct DNA sequencing. 
E) based on evolutionary relationships.

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

In the Agrobacterium Ti plasmid, the ____ gene codes for the synthesis of amino acids that are used as food.

When the lacZ gene technique is used to detect recombinant DNA in host bacterial cells, the colonies will appear _______ if the cells have been successfully transformed.

The Taq polymerase is used in recombinant DNA technology to:

Which of the following is NOT one of the goals of functional genomics?

Micropropagation is used to obtain clones of plants.

A knockout mutant has a particular gene inactivated.

One advantage of meristem culture is that virus-free plants can be obtained.

Why would a gene for cytokinin synthesis be fused to a promoter that is expressed only in senescent leaves?

The transcriptome refers to all of the:

In recombinant DNA technology, reverse transcriptase is used to:

Suppose a plasmid containing a gene of interest plus the ampR gene is used to transform E.coli cells.When these cells are placed on a medium containing ampicillin, what will happen?

DNA ligase would most likely be used in recombinant DNA technology to:

Because meristems lack _________ tissues, meristem culture results in plants that lack _________.

Traditional breeding programs:

Which of the following statements about particle bombardment is FALSE?

The polymerase chain reaction is used to:

Agrobacterium tumefaciens is used to obtain transgenetic plants.

One of the risks associated with genetic engineering is the spread of genes to weedy relatives by hybridization.

Glyphosate is:

A genetic map is different from a physical map in that a genetic map is:

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

Suppose a plasmid containing a gene of interest plus the amp^R gene is used to transform E.coli cells.When these cells are placed on a medium containing ampicillin, what will happen?

Because meristems lack _ tissues, meristem culture results in plants that lack _.