Question 1

Most of the genes in all bacteria are significantly similar.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following statements concerning DNA libraries is FALSE?

Accepted Answer

A) A genomic library is obtained by cutting the entire genome of an organism. 
B) A cDNA library is obtained by using reverse transcriptase. 
C) A DNA library contains cloned fragments of most of an organism's genome. 
D) A cDNA library lacks introns. 
E) A genomic library lacks exons. 
A) A genomic library is obtained by cutting the entire genome of an organism. 
B) A cDNA library is obtained by using reverse transcriptase. 
C) A DNA library contains cloned fragments of most of an organism's genome. 
D) A cDNA library lacks introns. 
E) A genomic library lacks exons.

Question 3

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

Accepted Answer

A) True 
 B)False

Question 4

Genetically modified plants were first grown commercially in:

Accepted Answer

A) 1965. 
B) 1985. 
C) 1996. 
D) 2001. 
E) 2009. 
A) 1965. 
B) 1985. 
C) 1996. 
D) 2001. 
E) 2009.

Question 5

One important characteristic the Taq polymerase is its

Accepted Answer

A) insensitivity to pH. 
B) ability to glow when in the presence of a suitable substrate. 
C) ability to be cloned. 
D) resistance to high temperature. 
E) ability to transform cells. 
A) insensitivity to pH. 
B) ability to glow when in the presence of a suitable substrate. 
C) ability to be cloned. 
D) resistance to high temperature. 
E) ability to transform cells.

Question 6

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

Accepted Answer

A) amplify the DNA molecule. 
B) carry out DNA hybridization. 
C) locate a gene of interest. 
D) determine the function of a gene. 
E) determine the nucleotide sequence of a gene. 
A) amplify the DNA molecule. 
B) carry out DNA hybridization. 
C) locate a gene of interest. 
D) determine the function of a gene. 
E) determine the nucleotide sequence of a gene.

Question 7

The advantage of using the "ice-minus" strain of Pseudomonas syringae is:

Accepted Answer

A) reducing susceptibility to fungi. 
B) increasing resistance to bacteria that thrive at low temperatures. 
C) increasing resistance to herbicides. 
D) reducing susceptibility to frost. 
E) increasing resistance to insect larvae. 
A) reducing susceptibility to fungi. 
B) increasing resistance to bacteria that thrive at low temperatures. 
C) increasing resistance to herbicides. 
D) reducing susceptibility to frost. 
E) increasing resistance to insect larvae.

Question 8

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Accepted Answer

A) True 
 B)False

Question 9

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

Accepted Answer

A) True 
 B)False

Question 10

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Accepted Answer

A) True 
 B)False

Question 11

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Accepted Answer

A) CG; CG-DNA 
B) O; R-DNA 
C) C; C-DNA 
D) Ti; T-DNA 
E) T-DNA; Ti 
A) CG; CG-DNA 
B) O; R-DNA 
C) C; C-DNA 
D) Ti; T-DNA 
E) T-DNA; Ti

Question 12

Which of the following statements about prokaryotic genomes is FALSE?

Accepted Answer

A) Most consist of a single circular chromosome. 
B) Approximately 90% of the identified genes have been assigned a function. 
C) About 25% of the genes have no significant similarity to other known genes in bacteria. 
D) Horizontal gene exchange periodically occurs. 
E) Gene transfers have occurred between bacteria and eukaryotes. 
A) Most consist of a single circular chromosome. 
B) Approximately 90% of the identified genes have been assigned a function. 
C) About 25% of the genes have no significant similarity to other known genes in bacteria. 
D) Horizontal gene exchange periodically occurs. 
E) Gene transfers have occurred between bacteria and eukaryotes.

Question 13

Which of the following statements concerning vectors is FALSE?

Accepted Answer

A) Vectors are used to transform cells. 
B) Vectors function as DNA carriers. 
C) A virus can be a vector. 
D) A plasmid can be a vector. 
E) A host cell can be a vector. 
A) Vectors are used to transform cells. 
B) Vectors function as DNA carriers. 
C) A virus can be a vector. 
D) A plasmid can be a vector. 
E) A host cell can be a vector.

Question 14

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

Accepted Answer

A) auxin synthesis only. 
B) opine synthesis only. 
C) cytokinin synthesis only. 
D) auxin and cytokinin synthesis. 
E) auxin and opine synthesis. 
A) auxin synthesis only. 
B) opine synthesis only. 
C) cytokinin synthesis only. 
D) auxin and cytokinin synthesis. 
E) auxin and opine synthesis.

Question 15

The proteome refers to all the protein molecules transcribed by the DNA.

Accepted Answer

A) True 
 B)False

Question 16

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Accepted Answer

A) True 
 B)False

Question 17

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Accepted Answer

A) True 
 B)False

Question 18

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

Accepted Answer

A) particle bombardment 
B) the polymerase chain reaction 
C) a reaction involving reverse transcriptase 
D) electrophoresis 
E) electroporation 
A) particle bombardment 
B) the polymerase chain reaction 
C) a reaction involving reverse transcriptase 
D) electrophoresis 
E) electroporation

Question 19

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

Accepted Answer

A) True 
 B)False

Question 20

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Accepted Answer

A) True 
 B)False

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

Most of the genes in all bacteria are significantly similar.

Which of the following statements concerning DNA libraries is FALSE?

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

Genetically modified plants were first grown commercially in:

One important characteristic the Taq polymerase is its

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

The advantage of using the "ice-minus" strain of Pseudomonas syringae is:

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Which of the following statements about prokaryotic genomes is FALSE?

Which of the following statements concerning vectors is FALSE?

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

The proteome refers to all the protein molecules transcribed by the DNA.

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Botany: an Introduction

The Molecular Composition of Plant Cells

The Plant Cell and the Cell Cycle

The Movement of Substances Into and Out of Cells

The Flow of Energy

Respiration

Photosynthesis, Light, and Life

Sexual Reproduction and Heredity

The Chemistry of Heredity and Gene Expression

The Process of Evolution

Systematics: the Science of Biological Diversity

Prokaryotes and Viruses

Fungi

Protists: Algae and Heterotrophic Protists

Bryophytes

Seedless Vascular Plants

Gymnosperms

Introduction to the Angiosperms

Evolution of the Angiosperms

Plants and People

Early Development of the Plant Body

Cells and Tissues of the Plant Body

The Root: Structure and Development

The Shoot: Primary Structure and Development

Secondary Growth in Stems

Regulating Growth and Development: the Plant Hormones

External Factors and Plant Growth

Plant Nutrition and Soils

The Movement of Water and Solutes in Plants

The Dynamics of Communities and Ecosystems

Global Ecology

Filters

Agrobacterium induces the formation of crown-gall tumors by transferring the plasmid containing __ to the host plant's nuclear DNA.