Question 1

Transcription in eukaryotic cells which is resistant to α-amanitin is carried out by RNA polymerase:

Accepted Answer

A)  I only. 
B)  II only. 
C)  III only. 
D)  I and III. 
E)  I, II and III. 
A)  I only. 
B)  II only. 
C)  III only. 
D)  I and III. 
E)  I, II and III.

Question 2

Describe the structure of an exosome.​

Accepted Answer

The catalytically active component of an

Question 3

Both prokaryotes and differentiated eukaryotic cells express ____ of their genes at any given time in the process called ____.

Accepted Answer

A)  a small number; transcription 
B)  a small number; translation 
C)  a large number; transcription 
D)  all; translation 
E)  all; transcription 
A)  a small number; transcription 
B)  a small number; translation 
C)  a large number; transcription 
D)  all; translation 
E)  all; transcription

Question 4

Spliceosomes include all of the following EXCEPT:

Accepted Answer

A)  snRNPs. 
B)  RNA-annealing proteins. 
C)  ATP-dependent RNA-unwinding proteins. 
D)  enzymes catalyzing transesterification of phosphoesters. 
E)  all are components. 
A)  snRNPs. 
B)  RNA-annealing proteins. 
C)  ATP-dependent RNA-unwinding proteins. 
D)  enzymes catalyzing transesterification of phosphoesters. 
E)  all are components.

Question 5

All are characteristics of catabolic activator protein (CAP) EXCEPT:

Accepted Answer

A)  ensures that the operons necessary for metabolism for alternate energy sources remains repressed until the supply of glucose is exhausted. 
B)  overrides the influence of any inducer that might be present. 
C)  cAMP enhances CAP's affinity for DNA. 
D)  N-terminal end binds cAMP and C-terminal end binds DNA. 
E)  all are true. 
A)  ensures that the operons necessary for metabolism for alternate energy sources remains repressed until the supply of glucose is exhausted. 
B)  overrides the influence of any inducer that might be present. 
C)  cAMP enhances CAP's affinity for DNA. 
D)  N-terminal end binds cAMP and C-terminal end binds DNA. 
E)  all are true.

Question 6

The base in DNA that specifies the first base in the RNA transcript is called a(n) ____ and is numbered ____.

Accepted Answer

A)  promoter; −5 
B)  enhancer; −35 
C)  transcription start site; 0 
D)  transcription start site; +1 
E)  promoter complex; +5 
A)  promoter; −5 
B)  enhancer; −35 
C)  transcription start site; 0 
D)  transcription start site; +1 
E)  promoter complex; +5

Question 7

The production of multiple transcripts from a single gene includes all EXCEPT:

Accepted Answer

A)  constitutive splicing. 
B)  use of different promoters. 
C)  selection of different polyadenylation sites. 
D)  alternative splicing of the primary transcript. 
E)  all are true. 
A)  constitutive splicing. 
B)  use of different promoters. 
C)  selection of different polyadenylation sites. 
D)  alternative splicing of the primary transcript. 
E)  all are true.

Question 8

All are true for the prokaryotic promoter for the rrn gene in E. coli EXCEPT that it:

Accepted Answer

A)  encodes for rRNA. 
B)  has a −10 region Pribnow box. 
C)  has a −35 region consensus sequence. 
D)  has an upstream (UP) element at −55. 
E)  all are true. 
A)  encodes for rRNA. 
B)  has a −10 region Pribnow box. 
C)  has a −35 region consensus sequence. 
D)  has an upstream (UP) element at −55. 
E)  all are true.

Question 9

The stand of dsDNA that is read by RNA polymerase is termed the ____ strand with RNA polymerase moving ____ along it. The polymerase forms a ____, growing in the ____ direction.

Accepted Answer

A)  template; 5'→3'; transcript; 3'→5' 
B)  template; 3'→5'; transcript; 5'→3' 
C)  transcript; 3'→5'; template; 5'→3' 
D)  nontemplate; 5'→3'; template; 3'→5' 
E)  nontemplate; 3'→5'; template; 5'→3' 
A)  template; 5'→3'; transcript; 3'→5' 
B)  template; 3'→5'; transcript; 5'→3' 
C)  transcript; 3'→5'; template; 5'→3' 
D)  nontemplate; 5'→3'; template; 3'→5' 
E)  nontemplate; 3'→5'; template; 5'→3'

Question 10

The recurring structural feature in DNA-binding proteins is the presence of ____ segments that fit into the ____ grove of B-DNA.

Accepted Answer

A)  α-helix; major 
B)  β-sheet; major 
C)  β-turn; minor 
D)  β-barrel; minor 
E)  all are true 
A)  α-helix; major 
B)  β-sheet; major 
C)  β-turn; minor 
D)  β-barrel; minor 
E)  all are true

Question 11

The structural motif found in a number of transcriptional factors which is not a DNA-binding domain, but rather is associated with protein-protein interactions is:

Accepted Answer

A)  a leucine zipper. 
B)  a zinc finger. 
C)  a helix-turn-helix. 
D)  an enhancer element. 
E)  all of the above. 
A)  a leucine zipper. 
B)  a zinc finger. 
C)  a helix-turn-helix. 
D)  an enhancer element. 
E)  all of the above.

Question 12

Which of the following is required for RNA splicing to occur?

Accepted Answer

A)  a free 5´ hydroxyl created by hydrolysis of the phosphate ester bond 
B)  a cyclic phospodiester at the 3´ end of the RNA molecule 
C)  a 2´ hydroxyl group 
D)  the enzyme splicase 
E)  none of the above 
A)  a free 5´ hydroxyl created by hydrolysis of the phosphate ester bond 
B)  a cyclic phospodiester at the 3´ end of the RNA molecule 
C)  a 2´ hydroxyl group 
D)  the enzyme splicase 
E)  none of the above

Question 13

All are lac repressor properties EXCEPT:

Accepted Answer

A)  tetrameric protein expressed constitutively. 
B)  binding site for DNA. 
C)  binding site for mRNA. 
D)  binding site for inducer. 
E)  blocks elongation so initiation is aborted. 
A)  tetrameric protein expressed constitutively. 
B)  binding site for DNA. 
C)  binding site for mRNA. 
D)  binding site for inducer. 
E)  blocks elongation so initiation is aborted.

Question 14

All may be RNA polymerase II promoter constituents EXCEPT:

Accepted Answer

A)  the core element where general transcription factors (GTFs) bind. 
B)  where enhancers bind transcriptional activators. 
C)  where silencers bind repressors. 
D)  a TATA box indicating the transcription start site. 
E)  all can be constituents. 
A)  the core element where general transcription factors (GTFs) bind. 
B)  where enhancers bind transcriptional activators. 
C)  where silencers bind repressors. 
D)  a TATA box indicating the transcription start site. 
E)  all can be constituents.

Question 15

During RNA chain elongation gyrase proceeds ahead of the transcription bubble in order to _____.

Accepted Answer

A)  remove negative supercoils by introducing positive supercoils 
B)  remove positive supercoils by introducing negative supercoils 
C)  remove negative supercoils by creating relaxed DNA 
D)  remove positive supercoils by creating relaxed DNA 
E)  none of the above 
A)  remove negative supercoils by introducing positive supercoils 
B)  remove positive supercoils by introducing negative supercoils 
C)  remove negative supercoils by creating relaxed DNA 
D)  remove positive supercoils by creating relaxed DNA 
E)  none of the above

Question 16

All are true for homeobox domains EXCEPT:

Accepted Answer

A)  typically comprise about 90% of the protein mass. 
B)  found in virtually every eukaryote. 
C)  act as sequence-specific transcriptional factors. 
D)  contain helix-turn-helix motifs. 
E)  all are true. 
A)  typically comprise about 90% of the protein mass. 
B)  found in virtually every eukaryote. 
C)  act as sequence-specific transcriptional factors. 
D)  contain helix-turn-helix motifs. 
E)  all are true.

Question 17

Nucleotides in the "plus" direction are said to lie ____ the transcription start site.

Accepted Answer

A)  downstrand of 
B)  upstream of 
C)  downstream of 
D)  upstrand of 
E)  positive to 
A)  downstrand of 
B)  upstream of 
C)  downstream of 
D)  upstrand of 
E)  positive to

Question 18

In bacteria, single polycistronic mRNA encodes for:

Accepted Answer

A)  a single enzyme although there are many in the metabolic pathway. 
B)  multiple enzymes of the particular pathway. 
C)  a single mRNA which is split into multiple mRNAs before translation. 
D)  a single peptide with multiple catabolic sites. 
E)  none of the above. 
A)  a single enzyme although there are many in the metabolic pathway. 
B)  multiple enzymes of the particular pathway. 
C)  a single mRNA which is split into multiple mRNAs before translation. 
D)  a single peptide with multiple catabolic sites. 
E)  none of the above.

Question 19

Huge multi-subunit entities that mediate ATP-dependent conformational changes that peel 50bp of DNA from the edge of nucleosome core particles and create a "bulge" in the DNA:core nucleosomes association are called:

Accepted Answer

A)  histone deacetylase complexes (HDACs). 
B)  DNA-bound transcriptional activators. 
C)  chromatin-remodeling complexes . 
D)  general transcriptional factors (GTFs). 
E)  all are true. 
A)  histone deacetylase complexes (HDACs). 
B)  DNA-bound transcriptional activators. 
C)  chromatin-remodeling complexes . 
D)  general transcriptional factors (GTFs). 
E)  all are true.

Question 20

Which is/are true of protein isoforms?

Accepted Answer

A)  they are related polypeptides 
B)  they have identical structure and function 
C)  they are the result of different transcripts of a single gene 
D)  a and c 
E)  a, b, and c 
A)  they are related polypeptides 
B)  they have identical structure and function 
C)  they are the result of different transcripts of a single gene 
D)  a and c 
E)  a, b, and c

Exam 29: Transcription and the Regulation of Gene Expression

Transcription in eukaryotic cells which is resistant to α-amanitin is carried out by RNA polymerase:

Describe the structure of an exosome.​

Both prokaryotes and differentiated eukaryotic cells express ____ of their genes at any given time in the process called ____.

Spliceosomes include all of the following EXCEPT:

All are characteristics of catabolic activator protein (CAP) EXCEPT:

The base in DNA that specifies the first base in the RNA transcript is called a(n) ____ and is numbered ____.

The production of multiple transcripts from a single gene includes all EXCEPT:

All are true for the prokaryotic promoter for the rrn gene in E. coli EXCEPT that it:

The stand of dsDNA that is read by RNA polymerase is termed the ____ strand with RNA polymerase moving ____ along it. The polymerase forms a ____, growing in the ____ direction.

The recurring structural feature in DNA-binding proteins is the presence of ____ segments that fit into the ____ grove of B-DNA.

The structural motif found in a number of transcriptional factors which is not a DNA-binding domain, but rather is associated with protein-protein interactions is:

Which of the following is required for RNA splicing to occur?

All are lac repressor properties EXCEPT:

All may be RNA polymerase II promoter constituents EXCEPT:

During RNA chain elongation gyrase proceeds ahead of the transcription bubble in order to _____.

All are true for homeobox domains EXCEPT:

Nucleotides in the "plus" direction are said to lie ____ the transcription start site.

In bacteria, single polycistronic mRNA encodes for:

Huge multi-subunit entities that mediate ATP-dependent conformational changes that peel 50bp of DNA from the edge of nucleosome core particles and create a "bulge" in the DNA:core nucleosomes association are called:

Which is/are true of protein isoforms?

The Facts of Life: Chemistry Is the Logic of Biological Phenomena

Water: the Medium of Life

Thermodynamics of Biological Systems

Amino Acids and the Peptide Bond

Proteins: Their Primary Structure and Biological Functions

Proteins: Secondary, Tertiary, and Quaternary Structure

Carbohydrates and the Glycoconjugates of Cell Surfaces

Lipids

Membranes and Membrane Transport

Nucleotides and Nucleic Acids

Structure of Nucleic Acids

Recombinant Dna, Cloning, Chimeric Genes, and Synthetic Biology

Enzymeskinetics and Specificity

Mechanisms of Enzyme Action

Enzyme Regulation

Molecular Motors

Metabolism: an Overview

Glycolysis

The Tricarboxylic Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

Fatty Acid Catabolism

Lipid Biosynthesis

Nitrogen Acquisition and Amino Acid Metabolism

Synthesis and Degradation of Nucleotides

Metabolic Integration and Organ Specialization

Dna Metabolism: Replication, Recombination, and Repair

Protein Synthesis

Completing the Protein Life Cycle: Folding, Processing, and Degradation

The Reception and Transmission of Extracellular Information

Filters

Describe the structure of an exosome.

Both prokaryotes and differentiated eukaryotic cells express of their genes at any given time in the process called .

The base in DNA that specifies the first base in the RNA transcript is called a(n) and is numbered .

The stand of dsDNA that is read by RNA polymerase is termed the strand with RNA polymerase moving along it. The polymerase forms a , growing in the direction.

The recurring structural feature in DNA-binding proteins is the presence of segments that fit into the grove of B-DNA.