Question 1

Which of the following DNA sequences is MOST likely to be bound by a steroid receptor?

Accepted Answer

A)  AGGAGAACATCATGTTCT 
B)  AGGAGATAGGAGAACT 
C)  TTTGATCCAGTTTCCAGT 
D)  CCCAAGTTCCCAAG 
A)  AGGAGAACATCATGTTCT 
B)  AGGAGATAGGAGAACT 
C)  TTTGATCCAGTTTCCAGT 
D)  CCCAAGTTCCCAAG

Question 2

Below is a figure of   protein bound to   protein.What is the name of the portion indicated by the arrow,and which protein is it a part of?

Accepted Answer

It is the switch II

Question 3

Consider a mutation in procaspase 3 that changes Asp28 to an amino acid that is no longer a substrate for autocleavage.This mutant would

Accepted Answer

A)  no longer be cleaved by caspase 8. 
B)  no longer obtain a quaternary structure. 
C)  be partially activated. 
D)  be fully activated. 
A)  no longer be cleaved by caspase 8. 
B)  no longer obtain a quaternary structure. 
C)  be partially activated. 
D)  be fully activated.

Question 4

Protein kinase A is immunoprecipitated from two different liver samples.The immunoprecipitate is then analyzed with SDS-PAGE.The first sample shows a single band on the gel,whereas the second displays two bands.Propose an explanation for why the results are different.

Accepted Answer

Protein kinase A can be bound by regulat

Question 5

A class of proteins that assist other proteins to fold are known as __________ proteins.

Accepted Answer

A)  folding assist 
B)  proteasome 
C)  chaperonin 
D)  overseer 
A)  folding assist 
B)  proteasome 
C)  chaperonin 
D)  overseer

Question 6

Explain how the conformational organization of a nuclear receptor homodimer allows it to recognize inverted repeat sequences of DNA.

Accepted Answer

The homodimer is organized hea

Question 7

The insulin receptor is a type of

Accepted Answer

A)  G protein-linked receptor. 
B)  receptor tyrosine kinase. 
C)  oncogene. 
D)  MEK. 
A)  G protein-linked receptor. 
B)  receptor tyrosine kinase. 
C)  oncogene. 
D)  MEK.

Question 8

This Zn2+-containing homodimer is part of

Accepted Answer

A)  the glucocorticoid receptor. 
B)  PPAR  -RXR   
C)  TRADD. 
D)  the retinoic acid receptor. 
A)  the glucocorticoid receptor. 
B)  PPAR  -RXR   
C)  TRADD. 
D)  the retinoic acid receptor.

Question 9

Which of the following is NOT an expected effect of the binding of the glucocorticoid response element by GR?

Accepted Answer

A)  upregulation of annexin I 
B)  inhibition of the inflammatory response 
C)  upregulation of cyclooxygenase-2 
D)  decreased interaction of p65 and p50 of NF$\kappa$B 
A)  upregulation of annexin I 
B)  inhibition of the inflammatory response 
C)  upregulation of cyclooxygenase-2 
D)  decreased interaction of p65 and p50 of NF$\kappa$B

Question 10

Which protein is part of the TNF receptor-activated programmed cell death signaling pathway?

Accepted Answer

A)  FADD 
B)  TRAF2 
C)  NF$\kappa$B-inducing kinase 
D)  IKK 
A)  FADD 
B)  TRAF2 
C)  NF$\kappa$B-inducing kinase 
D)  IKK

Question 11

Define the main characteristics of a signal transduction pathway and give a specific example of such a pathway.

Accepted Answer

A signal transduction pathway is a linke

Question 12

The result of TRADD proteins binding to TNF receptors is that

Accepted Answer

A)  TRADD is phosphorylated. 
B)  an activated adaptor complex is formed. 
C)  downstream signaling proteins are inactivated. 
D)  TNF is degraded. 
A)  TRADD is phosphorylated. 
B)  an activated adaptor complex is formed. 
C)  downstream signaling proteins are inactivated. 
D)  TNF is degraded.

Question 13

Removal of which metal ion would likely alter the structure of the GR DNA-binding domain and preclude it from binding to DNA?

Accepted Answer

A)  Zn2+ 
B)  Mg2+ 
C)  Ca2+ 
D)  Fe2+ 
A)  Zn2+ 
B)  Mg2+ 
C)  Ca2+ 
D)  Fe2+

Question 14

Below is a schematic representation of a dimerized receptor tyrosine kinase.Where is the kinase function located?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 15

Acetylcholine is a neurotransmitter that stimulates muscle contraction.Acetylcholine is acting as a(n)

Accepted Answer

A)  endocrine signal. 
B)  paracrine signal. 
C)  autocrine signal. 
D)  second messenger. 
A)  endocrine signal. 
B)  paracrine signal. 
C)  autocrine signal. 
D)  second messenger.

Question 16

If there were a technique that allowed one to isolate EGFR1 and EGFR2 at discrete steps along their activation pathway,which of the following would be isolated?

Accepted Answer

A)  A dimer in which EGFR2 contains phosphotyrosines but EGFR1 does not. 
B)  A dimer in which EGFR1 contains phosphotyrosines but EGFR2 does not. 
C)  A monomer of EGFR1 that contains phosphotyrosines. 
D)  A monomer of EGFR2 that contains phosphotyrosines. 
A)  A dimer in which EGFR2 contains phosphotyrosines but EGFR1 does not. 
B)  A dimer in which EGFR1 contains phosphotyrosines but EGFR2 does not. 
C)  A monomer of EGFR1 that contains phosphotyrosines. 
D)  A monomer of EGFR2 that contains phosphotyrosines.

Question 17

GRB2 contains both SH2 and SH3 domains.Contrast the role of each domain in the function of GRB2.

Accepted Answer

The SH2 domain of GRB2 identif

Question 18

A caspase is a

Accepted Answer

A)  protease. 
B)  kinase. 
C)  phosphatase. 
D)  transmembrane receptor. 
A)  protease. 
B)  kinase. 
C)  phosphatase. 
D)  transmembrane receptor.

Question 19

Which of the following is an example of a first or second messenger?

Accepted Answer

A)  peptide hormone 
B)  G protein-coupled receptor 
C)  protein kinase 
D)  cyclic nucleotide phosphodiesterase 
A)  peptide hormone 
B)  G protein-coupled receptor 
C)  protein kinase 
D)  cyclic nucleotide phosphodiesterase

Question 20

Construct a functional pathway out of the following steps. A: Phosphorylation of  Subunits of the insulin receptor occurs.B: Insulin binds to  Subunits of the insulin receptor.
C: PI-3K is activated.D: IRS is phosphorylated.

Accepted Answer

A)  C, A, B, D 
B)  B, D, A, C 
C)  B, A, C, D 
D)  B, A, D, C 
A)  C, A, B, D 
B)  B, D, A, C 
C)  B, A, C, D 
D)  B, A, D, C

Exam 8: Cell Signaling Systems

Which of the following DNA sequences is MOST likely to be bound by a steroid receptor?

Below is a figure of protein bound to protein.What is the name of the portion indicated by the arrow,and which protein is it a part of?

Consider a mutation in procaspase 3 that changes Asp28 to an amino acid that is no longer a substrate for autocleavage.This mutant would

Protein kinase A is immunoprecipitated from two different liver samples.The immunoprecipitate is then analyzed with SDS-PAGE.The first sample shows a single band on the gel,whereas the second displays two bands.Propose an explanation for why the results are different.

A class of proteins that assist other proteins to fold are known as __________ proteins.

Explain how the conformational organization of a nuclear receptor homodimer allows it to recognize inverted repeat sequences of DNA.

The insulin receptor is a type of

This Zn2+-containing homodimer is part of

Which of the following is NOT an expected effect of the binding of the glucocorticoid response element by GR?

Which protein is part of the TNF receptor-activated programmed cell death signaling pathway?

Define the main characteristics of a signal transduction pathway and give a specific example of such a pathway.

The result of TRADD proteins binding to TNF receptors is that

Removal of which metal ion would likely alter the structure of the GR DNA-binding domain and preclude it from binding to DNA?

Below is a schematic representation of a dimerized receptor tyrosine kinase.Where is the kinase function located?

Acetylcholine is a neurotransmitter that stimulates muscle contraction.Acetylcholine is acting as a(n)

If there were a technique that allowed one to isolate EGFR1 and EGFR2 at discrete steps along their activation pathway,which of the following would be isolated?

GRB2 contains both SH2 and SH3 domains.Contrast the role of each domain in the function of GRB2.

A caspase is a

Which of the following is an example of a first or second messenger?

Construct a functional pathway out of the following steps. A: Phosphorylation of Subunits of the insulin receptor occurs.B: Insulin binds to Subunits of the insulin receptor. C: PI-3K is activated.D: IRS is phosphorylated.

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

This Zn²⁺-containing homodimer is part of