Question 1

Table 6.2 The following data show growth of two bacteria on different media.
 
The data in Table 6.2 indicate that S.aureus is a(n)

Accepted Answer

A) mesophile. 
B) facultative anaerobe. 
C) facultative halophile. 
D) aerobe. 
E) halophile. 
A) mesophile. 
B) facultative anaerobe. 
C) facultative halophile. 
D) aerobe. 
E) halophile.

Question 2

Which of the following is an example of a metabolic activity that could be used to measure microbial growth?

Accepted Answer

A) standard plate count 
B) glucose consumption 
C) direct microscopic count 
D) turbidity 
E) most probable number (MPN) 
A) standard plate count 
B) glucose consumption 
C) direct microscopic count 
D) turbidity 
E) most probable number (MPN)

Question 3

Table 6.1
Three different culture media are shown below.
 $$\begin{array} { | c | c | c | } 
\hline \text { Medium A } & \text { Medium B } & \text { Medium C } \
\hline \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Yeast extract } & \text { Glucose } \
\hline \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \text { Beef extract } & \text { Peptone } \
\hline \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } \
\hline \mathrm { CaC } _ { 2 } & \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 2 } \
\hline \mathrm { NaHCO } _ { 3 } & ( \mathrm { NH } 4 ) _ { 2 } \mathrm { SO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } \
\hline
\end{array}$$
-For the three types of media in Table 6.1,which medium (or media)is/are chemically defined?

Accepted Answer

A) A 
B) B 
C) C 
D) A and B 
E) A and C 
A) A 
B) B 
C) C 
D) A and B 
E) A and C

Question 4

The term aerotolerant anaerobe refers to an organism that

Accepted Answer

A) does not use oxygen but tolerates it. 
B) is killed by oxygen. 
C) tolerates normal atmospheric nitrogen gas levels. 
D) requires less oxygen than is present in air. 
E) requires more oxygen than is present in air. 
A) does not use oxygen but tolerates it. 
B) is killed by oxygen. 
C) tolerates normal atmospheric nitrogen gas levels. 
D) requires less oxygen than is present in air. 
E) requires more oxygen than is present in air.

Question 5

Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count.How many viable and culturable cells were in the original sample?

Accepted Answer

A) 4 per milliliter 
B) 9 per milliliter 
C) 18 per milliliter 
D) 36 per milliliter 
E) 72 per milliliter 
A) 4 per milliliter 
B) 9 per milliliter 
C) 18 per milliliter 
D) 36 per milliliter 
E) 72 per milliliter

Question 6

Which enzyme catalyzes the reaction: O₂- + O₂- + 2H⁺ → H₂O₂ + O₂?

Accepted Answer

A) catalase 
B) oxidase 
C) peroxidase 
D) superoxide dismutase 
A) catalase 
B) oxidase 
C) peroxidase 
D) superoxide dismutase

Question 7

The biosafety level (BSL)for most introductory microbiology laboratories is

Accepted Answer

A) BSL-1. 
B) BSL-2. 
C) BSL-3. 
D) BSL-4. 
A) BSL-1. 
B) BSL-2. 
C) BSL-3. 
D) BSL-4.

Question 8

Bacterial growth refers to an increase in the numbers of cells in a bacterial culture.

Accepted Answer

A) True 
 B)False

Question 9

You are tasked with attempting to culture microbes in the lab that were initially obtained from samples taken in and near the Arctic circle,in areas of extreme cold and either very long or very short days,depending on the time of year.How would you begin to try to grow these microbes in the lab,and why would you choose those methods?

Accepted Answer

To begin attempting to culture microbes

Question 10

The biosafety level (BSL)for a clinical microbiology laboratory working with potentially airborne pathogens,such as tuberculosis bacteria,is

Accepted Answer

A) BSL-1. 
B) BSL-2. 
C) BSL-3. 
D) BSL-4. 
A) BSL-1. 
B) BSL-2. 
C) BSL-3. 
D) BSL-4.

Question 11

An extreme-weather event has knocked out power in your community.Even with rebuilding efforts,it appears that it could be 6 months or longer before power is restored.Since you have a background in microbiology,the people you know are asking you for possible ways of storing/preserving their food safely without electricity.What advice can you give them,and why would you make those recommendations?

Accepted Answer

As a microbiologist, I can provide some

Question 12

An isolated colony on a streak plate contains millions (or even billions)of identical cells all arising from one initial cell.

Accepted Answer

A) True 
 B)False

Question 13

Assume you inoculated 100 cells,with a generation time of 20 minutes,into 100 ml of nutrient broth.You then inoculated 100 cells of the same species into 200 ml of nutrient broth.After incubation for 3 hours,you can reasonably expect to have

Accepted Answer

A) more cells in the 100 ml. 
B) more cells in the 200 ml. 
C) the same number of cells in both. 
D) The answer cannot be determined based on the information provided. 
A) more cells in the 100 ml. 
B) more cells in the 200 ml. 
C) the same number of cells in both. 
D) The answer cannot be determined based on the information provided.

Question 14

Gelatin is used as a solidifying agent in microbiological media since few bacteria can degrade it.

Accepted Answer

A) True 
 B)False

Question 15

Most bacteria grow best at pH

Accepted Answer

A) 1. 
B) 5. 
C) 7. 
D) 9. 
E) 14. 
A) 1. 
B) 5. 
C) 7. 
D) 9. 
E) 14.

Question 16

Table 6.1
Three different culture media are shown below.
 $$\begin{array} { | c | c | c | } 
\hline \text { Medium A } & \text { Medium B } & \text { Medium C } \
\hline \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } & \text { Yeast extract } & \text { Glucose } \
\hline \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \text { Beef extract } & \text { Peptone } \
\hline \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 4 } & \left( \mathrm { NH } _ { 4 } \right) _ { 2 } \mathrm { SO } _ { 4 } \
\hline \mathrm { CaC } _ { 2 } & \mathrm { MgSO } _ { 4 } & \mathrm { KH } _ { 2 } \mathrm { PO } _ { 2 } \
\hline \mathrm { NaHCO } _ { 3 } & ( \mathrm { NH } 4 ) _ { 2 } \mathrm { SO } _ { 4 } & \mathrm { Na } _ { 2 } \mathrm { HPO } _ { 4 } \
\hline
\end{array}$$
-In Table,6.1,in which medium (or media)would an autotroph grow but a heterotroph could not?

Accepted Answer

A) A 
B) B 
C) C 
D) A and B 
E) A and C 
A) A 
B) B 
C) C 
D) A and B 
E) A and C

Question 17

Most fungi grow best at pH

Accepted Answer

A) 1. 
B) 5. 
C) 7. 
D) 9. 
E) 14. 
A) 1. 
B) 5. 
C) 7. 
D) 9. 
E) 14.

Question 18

Which enzyme catalyzes the following reaction? 2H₂O2 → 2H₂O + O₂

Accepted Answer

A) catalase 
B) oxidase 
C) peroxidase 
D) superoxide dismutase 
A) catalase 
B) oxidase 
C) peroxidase 
D) superoxide dismutase

Question 19

Figure 6.2
  
-Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture.In the figure,which sections of the graph illustrate a logarithmic change in cell numbers?

Accepted Answer

A) a and c 
B) b and d 
C) a and b 
D) c and d 
E) a and d 
A) a and c 
B) b and d 
C) a and b 
D) c and d 
E) a and d

Question 20

During which growth phase will gram-positive bacteria be most susceptible to penicillin?

Accepted Answer

A) lag phase 
B) log phase 
C) death phase 
D) stationary phase 
E) The culture is equally susceptible during all phases. 
A) lag phase 
B) log phase 
C) death phase 
D) stationary phase 
E) The culture is equally susceptible during all phases.

Exam 6: Microbial Growth

Table 6.2 The following data show growth of two bacteria on different media. The data in Table 6.2 indicate that S.aureus is a(n)

Which of the following is an example of a metabolic activity that could be used to measure microbial growth?

Table 6.1 Three different culture media are shown below. Medium A Medium B Medium C Yeast extract Glucose Beef extract Peptone (4 -For the three types of media in Table 6.1,which medium (or media)is/are chemically defined?

The term aerotolerant anaerobe refers to an organism that

Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count.How many viable and culturable cells were in the original sample?

Which enzyme catalyzes the reaction: O₂- + O₂- + 2H⁺ → H₂O₂ + O₂?

The biosafety level (BSL)for most introductory microbiology laboratories is

Bacterial growth refers to an increase in the numbers of cells in a bacterial culture.

The biosafety level (BSL)for a clinical microbiology laboratory working with potentially airborne pathogens,such as tuberculosis bacteria,is

An isolated colony on a streak plate contains millions (or even billions)of identical cells all arising from one initial cell.

Assume you inoculated 100 cells,with a generation time of 20 minutes,into 100 ml of nutrient broth.You then inoculated 100 cells of the same species into 200 ml of nutrient broth.After incubation for 3 hours,you can reasonably expect to have

Gelatin is used as a solidifying agent in microbiological media since few bacteria can degrade it.

Most bacteria grow best at pH

Table 6.1 Three different culture media are shown below. Medium A Medium B Medium C Yeast extract Glucose Beef extract Peptone (4 -In Table,6.1,in which medium (or media)would an autotroph grow but a heterotroph could not?

Most fungi grow best at pH

Which enzyme catalyzes the following reaction? 2H₂O2 → 2H₂O + O₂

Figure 6.2 -Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture.In the figure,which sections of the graph illustrate a logarithmic change in cell numbers?

During which growth phase will gram-positive bacteria be most susceptible to penicillin?

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic

Microbial Metabolism

The Control of Microbial Growth

Microbial Genetics

Biotechnology and Dna Technology

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, algae, protozoa, and Helminths

Viruses,viroids,and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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