Exam 12: Protein Sorting and Transport

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The major model of vesicle fusion holds that actual fusion of a vesicle with its target membrane is driven by the interaction of pairs of proteins called vesicle and target

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Cargo proteins are transported through the Golgi apparatus to the trans-Golgi network, where they are sorted into various vesicles that are targeted to different destinations. What is the role of vesicles in cargo protein transport through the Golgi apparatus?

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The process by which cells degrade their own components by enclosing them in ER membrane is

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How do lysosomes become acidic?

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A member of the heat-shock class of proteins, BiP, is necessary for the proper folding of proteins in the ER. In terms of function, what do BiP and the heat-shock proteins (HSP) have in common?

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Describe the process of autophagy.

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When compared to polypeptides synthesized in vitro on free ribosomes, polypeptides synthesized from the same mRNA on microsome-bound ribosomes often are

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The pH inside lysosomes is about

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Phospholipids are synthesized in the cytosolic half of the ER bilayer.

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What are the two families of small GTP-binding proteins that are involved in the formation of coated vesicles?

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Smooth ER is abundant in cells actively synthesizing steroid hormones.

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In eukaryotic cells, most protein synthesis is initiated in the _______.

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Cargo proteins are transported through the Golgi apparatus by

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Describe the selective and nonselective cellular purposes of autophagy.

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The sequence Lys-Asp-Glu-Leu (KDEL) serves to retain proteins in the ER by

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Arf function on vesicles is regulated by

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Using radiolabeled amino acids, Palade and colleagues defined the pathway taken by secreted proteins. Which sequence represents that pathway?

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Proteins that fail to fold properly in the endoplasmic reticulum are removed through the process of _______.

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The cytosolic surface of the rough endoplasmic membrane can be readily distinguished from the luminal surface by the presence of _______.

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Free ribosomes in the cytosol and ribosomes bound to the ER membrane have

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