Exam 18: The Cell Cycle

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Phosphorylation of the Golgi matrix proteins by Cdk1 during mitosis

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Cell plate formation occurs only in _______ cells.

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The p21 and p15 proteins are examples of

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The passage of a cell through the stages of the cell cycle is controlled by protein kinases that phosphorylate many different proteins at appropriate times. What are these protein kinases called?

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Which of the following represents the amount of DNA in a typical G2 cell?

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Cells are restricted to one round of DNA replication per cycle by origin of replication binding proteins called

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Separation of anaphase chromosomes begins when _______ is degraded to break the link between sister chromatids.

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A mutant cyclin B that is resistant to degradation by protease, but is otherwise active, would block the metaphase-to-anaphase transition.

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Cytokinesis begins during the _______ stage of mitosis.

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Progression through the cell cycle in the yeast Saccharomyces cerevisiae is regulated by

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During G2, the Wee1 protein phosphorylates tyrosine-15 of the Cdc2 protein kinase, thus inactivating it. Only once dephosphorylation occurs and Cdc2 becomes active can the cell pass from G2 into M and proceed to cell division. Can you think of a reason why this protein was named Wee1?

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MPF is a

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The most variable phase of the cell cycle is _______.

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Maturation promotion factor (MPF), discovered by Masui and Markert, is the factor that

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Many cancer researchers and tumor biologists focus their studies on regulation of the cell cycle. Why?

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DNA content can be found using a method of flow cytometry that can determine 2n or 4n states of cells based on cell counting and fluorescent labeling of DNA. Which of the following phases would be identified through flow cytometry as 2n?

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MPF is a general regulator of the transition from

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To prevent cells with unreplicated DNA from passing through the G2 checkpoint, Chk1 and Chk2 _______ protein phosphatase Cdc25.

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Interphase is defined as

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During mitosis, the rate of microtubule turnover

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